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Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model.
J Korean Med Sci. 2015 Dec; 30(12):1902-10.JK

Abstract

Lumbar disc herniation is commonly encountered in clinical practice and can induce sciatica due to mechanical and/or chemical irritation and the release of proinflammatory cytokines. However, symptoms are not confined to the affected spinal cord segment. The purpose of this study was to determine whether multisegmental molecular changes exist between adjacent lumbar spinal segments using a rat model of lumbar disc herniation. Twenty-nine male Sprague-Dawley rats were randomly assigned to either a sham-operated group (n=10) or a nucleus pulposus (NP)-exposed group (n=19). Rats in the NP-exposed group were further subdivided into a significant pain subgroup (n=12) and a no significant pain subgroup (n=7) using mechanical pain thresholds determined von Frey filaments. Immunohistochemical stainings of microglia (ionized calcium-binding adapter molecule 1; Iba1), astrocytes (glial fibrillary acidic protein; GFAP), calcitonin gene-related peptide (CGRP), and transient receptor potential vanilloid 1 (TRPV1) was performed in spinal dorsal horns and dorsal root ganglions (DRGs) at 10 days after surgery. It was found immunoreactivity for Iba1-positive microglia was higher in the L5 (P=0.004) dorsal horn and in the ipsilateral L4 (P=0.009), L6 (P=0.002), and S1 (P=0.002) dorsal horns in the NP-exposed group than in the sham-operated group. The expression of CGRP was also significantly higher in ipsilateral L3, L4, L6, and S1 segments and in L5 DRGs at 10 days after surgery in the NP-exposed group than in the sham-operated group (P<0.001). Our results indicate that lumbar disc herniation upregulates microglial activity and CGRP expression in many adjacent and ipsilateral lumbar spinal segments.

Authors+Show Affiliations

Department of Physical Medicine and Rehabilitation, College of Medicine, Daegu Catholic University, Daegu, Korea.Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University, Daegu, Korea. ; Medical Devices Clinical Trial Center, Yeungnam University, Daegu, Korea.Medical Devices Clinical Trial Center, Yeungnam University, Daegu, Korea.Medical Devices Clinical Trial Center, Yeungnam University, Daegu, Korea.Department of Anatomy and Cell Biology, School of Medicine, Hanyang University, Seoul, Korea.Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University, Daegu, Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26713069

Citation

Cho, Hee Kyung, et al. "Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments After Lumbar Disc Herniation in a Rat Model." Journal of Korean Medical Science, vol. 30, no. 12, 2015, pp. 1902-10.
Cho HK, Ahn SH, Kim SY, et al. Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model. J Korean Med Sci. 2015;30(12):1902-10.
Cho, H. K., Ahn, S. H., Kim, S. Y., Choi, M. J., Hwang, S. J., & Cho, Y. W. (2015). Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model. Journal of Korean Medical Science, 30(12), 1902-10. https://doi.org/10.3346/jkms.2015.30.12.1902
Cho HK, et al. Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments After Lumbar Disc Herniation in a Rat Model. J Korean Med Sci. 2015;30(12):1902-10. PubMed PMID: 26713069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model. AU - Cho,Hee Kyung, AU - Ahn,Sang Ho, AU - Kim,So-Yeon, AU - Choi,Mi-Jung, AU - Hwang,Se Jin, AU - Cho,Yun Woo, Y1 - 2015/11/30/ PY - 2015/08/03/received PY - 2015/10/28/accepted PY - 2015/12/30/entrez PY - 2015/12/30/pubmed PY - 2016/10/8/medline KW - Calcitonin Gene-Related Peptide KW - Lumbar Disc Herniation KW - Microglia KW - Multisegmental KW - Neural Pathways KW - Neuropathic Pain SP - 1902 EP - 10 JF - Journal of Korean medical science JO - J Korean Med Sci VL - 30 IS - 12 N2 - Lumbar disc herniation is commonly encountered in clinical practice and can induce sciatica due to mechanical and/or chemical irritation and the release of proinflammatory cytokines. However, symptoms are not confined to the affected spinal cord segment. The purpose of this study was to determine whether multisegmental molecular changes exist between adjacent lumbar spinal segments using a rat model of lumbar disc herniation. Twenty-nine male Sprague-Dawley rats were randomly assigned to either a sham-operated group (n=10) or a nucleus pulposus (NP)-exposed group (n=19). Rats in the NP-exposed group were further subdivided into a significant pain subgroup (n=12) and a no significant pain subgroup (n=7) using mechanical pain thresholds determined von Frey filaments. Immunohistochemical stainings of microglia (ionized calcium-binding adapter molecule 1; Iba1), astrocytes (glial fibrillary acidic protein; GFAP), calcitonin gene-related peptide (CGRP), and transient receptor potential vanilloid 1 (TRPV1) was performed in spinal dorsal horns and dorsal root ganglions (DRGs) at 10 days after surgery. It was found immunoreactivity for Iba1-positive microglia was higher in the L5 (P=0.004) dorsal horn and in the ipsilateral L4 (P=0.009), L6 (P=0.002), and S1 (P=0.002) dorsal horns in the NP-exposed group than in the sham-operated group. The expression of CGRP was also significantly higher in ipsilateral L3, L4, L6, and S1 segments and in L5 DRGs at 10 days after surgery in the NP-exposed group than in the sham-operated group (P<0.001). Our results indicate that lumbar disc herniation upregulates microglial activity and CGRP expression in many adjacent and ipsilateral lumbar spinal segments. SN - 1598-6357 UR - https://www.unboundmedicine.com/medline/citation/26713069/Changes_in_the_Expressions_of_Iba1_and_Calcitonin_Gene_Related_Peptide_in_Adjacent_Lumbar_Spinal_Segments_after_Lumbar_Disc_Herniation_in_a_Rat_Model_ L2 - https://jkms.org/DOIx.php?id=10.3346/jkms.2015.30.12.1902 DB - PRIME DP - Unbound Medicine ER -