Tags

Type your tag names separated by a space and hit enter

Genetic cell targeting uncovers specific neuronal types and distinct subregions in the bed nucleus of the stria terminalis.
J Comp Neurol 2016; 524(12):2379-99JC

Abstract

The bed nucleus of the stria terminalis (BNST) plays an important role in fear, stress, and anxiety. It contains a collection of subnuclei delineated by gross cytoarchitecture features; however, there has yet to be a systematic examination of specific BNST neuronal types and their associated neurochemical makeup. The present study focuses on improved characterization of the anterior BNST based on differing molecular and chemical expression aided by mouse genetics. Specific Cre driver lines crossed with a fluorescent reporter line were used for genetic cell targeting and immunochemical staining. Using this new approach, we were able to robustly identify specific excitatory and inhibitory cell types in the BNST. The presence and distribution of excitatory neurons were firmly established; glutamatergic neurons in the anterior BNST accounted for about 14% and 31% of dorsal and ventral BNST cells, respectively. GABAergic neurons expressing different isoforms of glutamic acid decarboxylase were found to have differential subregional distributions. Almost no parvalbumin-expressing cells were found in the BNST, while somatostatin-expressing cells and calretinin-expressing cells account for modest proportions of BNST cells. In addition, vasoactive intestinal peptide-expressing axonal plexuses were prominent in the oval and juxtacapsular subregions. In addition, we discovered that corticotropin-releasing hormone-expressing cells contain GABAergic and glutamatergic subpopulations. Together, this study reveals new information on excitatory and inhibitory neurons in the BNST, which will facilitate genetic dissection and functional studies of BNST subregions. J. Comp. Neurol. 524:2379-2399, 2016. © 2016 Wiley Periodicals, Inc.

Authors+Show Affiliations

Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, California, USA.Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, California, USA.Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, California, USA.Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, California, USA.Department of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, California, USA. Department of Biomedical Engineering, University of California, Irvine, California, USA. Department of Microbiology and Molecular Genetics, University of California, Irvine, California, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26718312

Citation

Nguyen, Amanda Q., et al. "Genetic Cell Targeting Uncovers Specific Neuronal Types and Distinct Subregions in the Bed Nucleus of the Stria Terminalis." The Journal of Comparative Neurology, vol. 524, no. 12, 2016, pp. 2379-99.
Nguyen AQ, Dela Cruz JA, Sun Y, et al. Genetic cell targeting uncovers specific neuronal types and distinct subregions in the bed nucleus of the stria terminalis. J Comp Neurol. 2016;524(12):2379-99.
Nguyen, A. Q., Dela Cruz, J. A., Sun, Y., Holmes, T. C., & Xu, X. (2016). Genetic cell targeting uncovers specific neuronal types and distinct subregions in the bed nucleus of the stria terminalis. The Journal of Comparative Neurology, 524(12), pp. 2379-99. doi:10.1002/cne.23954.
Nguyen AQ, et al. Genetic Cell Targeting Uncovers Specific Neuronal Types and Distinct Subregions in the Bed Nucleus of the Stria Terminalis. J Comp Neurol. 2016 08 15;524(12):2379-99. PubMed PMID: 26718312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic cell targeting uncovers specific neuronal types and distinct subregions in the bed nucleus of the stria terminalis. AU - Nguyen,Amanda Q, AU - Dela Cruz,Julie A D, AU - Sun,Yanjun, AU - Holmes,Todd C, AU - Xu,Xiangmin, Y1 - 2016/01/26/ PY - 2015/03/19/received PY - 2015/12/23/revised PY - 2015/12/28/accepted PY - 2016/1/1/entrez PY - 2016/1/1/pubmed PY - 2017/9/14/medline KW - BNST KW - GABAergic KW - glutamatergic KW - immunochemical KW - subregions KW - transgenic SP - 2379 EP - 99 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 524 IS - 12 N2 - The bed nucleus of the stria terminalis (BNST) plays an important role in fear, stress, and anxiety. It contains a collection of subnuclei delineated by gross cytoarchitecture features; however, there has yet to be a systematic examination of specific BNST neuronal types and their associated neurochemical makeup. The present study focuses on improved characterization of the anterior BNST based on differing molecular and chemical expression aided by mouse genetics. Specific Cre driver lines crossed with a fluorescent reporter line were used for genetic cell targeting and immunochemical staining. Using this new approach, we were able to robustly identify specific excitatory and inhibitory cell types in the BNST. The presence and distribution of excitatory neurons were firmly established; glutamatergic neurons in the anterior BNST accounted for about 14% and 31% of dorsal and ventral BNST cells, respectively. GABAergic neurons expressing different isoforms of glutamic acid decarboxylase were found to have differential subregional distributions. Almost no parvalbumin-expressing cells were found in the BNST, while somatostatin-expressing cells and calretinin-expressing cells account for modest proportions of BNST cells. In addition, vasoactive intestinal peptide-expressing axonal plexuses were prominent in the oval and juxtacapsular subregions. In addition, we discovered that corticotropin-releasing hormone-expressing cells contain GABAergic and glutamatergic subpopulations. Together, this study reveals new information on excitatory and inhibitory neurons in the BNST, which will facilitate genetic dissection and functional studies of BNST subregions. J. Comp. Neurol. 524:2379-2399, 2016. © 2016 Wiley Periodicals, Inc. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/26718312/Genetic_cell_targeting_uncovers_specific_neuronal_types_and_distinct_subregions_in_the_bed_nucleus_of_the_stria_terminalis_ L2 - https://doi.org/10.1002/cne.23954 DB - PRIME DP - Unbound Medicine ER -