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Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment.
Bioresour Technol. 2016 Mar; 203:80-8.BT

Abstract

The synergistic effect of temperature (25-65 °C) and total specific energy input (0.55-1.11 kWh kgDW(-1)) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55 °C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55 °C. Above 55 °C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Postma PR
Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands. Electronic address: richard.postma@wur.nl.
Pataro G
Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II 132, Fisciano, SA, Italy. Electronic address: gpataro@unisa.it.
Capitoli M
ProdAl Scarl - University of Salerno, via Ponte don Melillo, 84084 Fisciano, SA, Italy.
Barbosa MJ
Food & Biobased Reseach, AlgaePARC, Wageningen University and Research Centre, PO Box 17, 6700 AA Wageningen, The Netherlands.
Wijffels RH
Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands; University of Nordland, Faculty of Biosciences and Aquaculture, N-8049 Bodø, Norway.
Eppink MH
Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
Olivieri G
Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
Ferrari G
Department of Industrial Engineering, University of Salerno, via Giovanni Paolo II 132, Fisciano, SA, Italy; ProdAl Scarl - University of Salerno, via Ponte don Melillo, 84084 Fisciano, SA, Italy.

MeSH

Algal ProteinsCarbohydratesCell MembraneChemical FractionationChlorella vulgarisElectricityElectrochemical TechniquesMicroalgaeRibulose-Bisphosphate CarboxylaseTemperature

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26722806

Citation

Postma, P R., et al. "Selective Extraction of Intracellular Components From the Microalga Chlorella Vulgaris By Combined Pulsed Electric Field-temperature Treatment." Bioresource Technology, vol. 203, 2016, pp. 80-8.
Postma PR, Pataro G, Capitoli M, et al. Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment. Bioresour Technol. 2016;203:80-8.
Postma, P. R., Pataro, G., Capitoli, M., Barbosa, M. J., Wijffels, R. H., Eppink, M. H., Olivieri, G., & Ferrari, G. (2016). Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment. Bioresource Technology, 203, 80-8. https://doi.org/10.1016/j.biortech.2015.12.012
Postma PR, et al. Selective Extraction of Intracellular Components From the Microalga Chlorella Vulgaris By Combined Pulsed Electric Field-temperature Treatment. Bioresour Technol. 2016;203:80-8. PubMed PMID: 26722806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment. AU - Postma,P R, AU - Pataro,G, AU - Capitoli,M, AU - Barbosa,M J, AU - Wijffels,R H, AU - Eppink,M H M, AU - Olivieri,G, AU - Ferrari,G, Y1 - 2015/12/15/ PY - 2015/10/05/received PY - 2015/11/30/revised PY - 2015/12/08/accepted PY - 2016/1/2/entrez PY - 2016/1/2/pubmed PY - 2017/1/11/medline KW - Bead mill KW - Microalgae KW - Mild cascade biorefinery KW - Pulsed electric field KW - Temperature SP - 80 EP - 8 JF - Bioresource technology JO - Bioresour Technol VL - 203 N2 - The synergistic effect of temperature (25-65 °C) and total specific energy input (0.55-1.11 kWh kgDW(-1)) by pulsed electric field (PEF) on the release of intracellular components from the microalgae Chlorella vulgaris was studied. The combination of PEF with temperatures from 25 to 55 °C resulted in a conductivity increase of 75% as a result of cell membrane permeabilization. In this range of temperatures, 25-39% carbohydrates and 3-5% proteins release occurred and only for carbohydrate release a synergistic effect was observed at 55 °C. Above 55 °C spontaneous cell lysis occurred without PEF. Combined PEF-temperature treatment does not sufficiently disintegrate the algal cells to release both carbohydrates and proteins at yields comparable to the benchmark bead milling (40-45% protein, 48-58% carbohydrates). SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/26722806/Selective_extraction_of_intracellular_components_from_the_microalga_Chlorella_vulgaris_by_combined_pulsed_electric_field_temperature_treatment_ DB - PRIME DP - Unbound Medicine ER -