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Antibacterial effects of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens.
J Ethnopharmacol. 2016 Feb 17; 179:76-82.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The leaves, stems and roots of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg. are used as traditional medicine in many African countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds.

AIM OF THE STUDY

To determine the in vitro antibacterial activity of the crude extracts of leaves and stems of A. cordifolia on gastrointestinal, skin, respiratory and urinary tract pathogens and to identify the compounds in the extracts that may be responsible for this activity.

MATERIALS AND METHODS

The antibacterial activities of crude extracts [hexane, chloroform (CHCl3), ethyl acetate (EtOAc), ethanol (EtOH), methanol (MeOH) and water (H2O)] as well as pure compounds isolated from these extracts were evaluated by means of the micro-dilution assay against four Gram-positive bacteria, i.e. Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923 and S. saprophyticus ATCC 15305, as well as four Gram-negative bacterial strains, i.e. Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Moraxella catarrhalis ATCC 23246 and Proteus mirabilis ATCC 43071. The isolation of the active constituents was undertaken by bio-autographic assays in conjunction with chromatographic techniques. The identification and characterisation of the isolated compounds were done using mass spectrometry (MS) and Fourier transformed infrared spectrometry (FTIR) as well as 1D- and 2D- nuclear magnetic resonance (NMR) analyses.

RESULTS

The leaves and stems of A. cordifolia exhibited varied antibacterial activity against all eight pathogens. Most of the MIC values ranged between 63 and 2000µg/ml. The highest activities for the crude extracts (63µg/ml) were observed against S. saprophyticus [stem (EtOAc, CHCl3 and hexane), leaves (MeOH, EtOH, EtOAc and CHCl3)], E. coli [stem (MeOH and EtOH), leaves (MeOH, EtOH, EtOAc and CHCl3)], M. catarrhalis [leaves (EtOAc and CHCl3)], K. pneumoniae [stem (CHCl3), leaves (CHCl3)] and S. aureus [leaves (CHCl3)]. Seven constituents [stigmasterol (1), stigmasta-4,22-dien-3-one (2), friedelin (3), friedelane-3-one-28-al (4), 3-O-acetyl-aleuritolic acid (5), 3-O-acetyl-erythrodiol (6) and methyl-3,4,5-trihydroxybenzoate (methyl gallate) (7)] were isolated from the stem MeOH extract. All these compounds displayed some antibacterial activity against the eight pathogens with highest activity against S. saprophyticus (2µg/ml). Furthermore, this is the first report of compounds 1, 2, 3, 4, 6 and 7 isolated from A. cordifolia and where a complete set of 2D-NMR data for fridelane-3-one-28-al (4) is presented.

CONCLUSION

The study demonstrated that the antibacterial activities of A. cordifolia extracts may be due to the presence of the seven isolated compounds, where compounds 3-6 showed the best activity. The observed activity against gastrointestinal, skin, respiratory and urinary tract pathogens supports the traditional use for the treatment of such ailments.

Authors+Show Affiliations

Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa. Electronic address: xavsiw@gmail.com.Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa.Department of Pharmacy and Pharmacology, University of the Witwatersrand, 7 York Rd, Parktown 2193, South Africa.Department of Applied Chemistry, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South Africa.Department of Pharmacy and Pharmacology, University of the Witwatersrand, 7 York Rd, Parktown 2193, South Africa.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26724423

Citation

Noundou, X Siwe, et al. "Antibacterial Effects of Alchornea Cordifolia (Schumach. and Thonn.) Müll. Arg Extracts and Compounds On Gastrointestinal, Skin, Respiratory and Urinary Tract Pathogens." Journal of Ethnopharmacology, vol. 179, 2016, pp. 76-82.
Noundou XS, Krause RW, van Vuuren SF, et al. Antibacterial effects of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. J Ethnopharmacol. 2016;179:76-82.
Noundou, X. S., Krause, R. W., van Vuuren, S. F., Ndinteh, D. T., & Olivier, D. K. (2016). Antibacterial effects of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. Journal of Ethnopharmacology, 179, 76-82. https://doi.org/10.1016/j.jep.2015.12.043
Noundou XS, et al. Antibacterial Effects of Alchornea Cordifolia (Schumach. and Thonn.) Müll. Arg Extracts and Compounds On Gastrointestinal, Skin, Respiratory and Urinary Tract Pathogens. J Ethnopharmacol. 2016 Feb 17;179:76-82. PubMed PMID: 26724423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial effects of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. AU - Noundou,X Siwe, AU - Krause,R W M, AU - van Vuuren,S F, AU - Ndinteh,D Tantoh, AU - Olivier,D K, Y1 - 2015/12/24/ PY - 2015/08/25/received PY - 2015/12/16/revised PY - 2015/12/20/accepted PY - 2016/1/3/entrez PY - 2016/1/3/pubmed PY - 2016/11/1/medline KW - Alchornea cordifolia KW - Antibacterial activity KW - Isolated compounds KW - Leaves KW - Stem SP - 76 EP - 82 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 179 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves, stems and roots of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg. are used as traditional medicine in many African countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. AIM OF THE STUDY: To determine the in vitro antibacterial activity of the crude extracts of leaves and stems of A. cordifolia on gastrointestinal, skin, respiratory and urinary tract pathogens and to identify the compounds in the extracts that may be responsible for this activity. MATERIALS AND METHODS: The antibacterial activities of crude extracts [hexane, chloroform (CHCl3), ethyl acetate (EtOAc), ethanol (EtOH), methanol (MeOH) and water (H2O)] as well as pure compounds isolated from these extracts were evaluated by means of the micro-dilution assay against four Gram-positive bacteria, i.e. Bacillus cereus ATCC 11778, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923 and S. saprophyticus ATCC 15305, as well as four Gram-negative bacterial strains, i.e. Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Moraxella catarrhalis ATCC 23246 and Proteus mirabilis ATCC 43071. The isolation of the active constituents was undertaken by bio-autographic assays in conjunction with chromatographic techniques. The identification and characterisation of the isolated compounds were done using mass spectrometry (MS) and Fourier transformed infrared spectrometry (FTIR) as well as 1D- and 2D- nuclear magnetic resonance (NMR) analyses. RESULTS: The leaves and stems of A. cordifolia exhibited varied antibacterial activity against all eight pathogens. Most of the MIC values ranged between 63 and 2000µg/ml. The highest activities for the crude extracts (63µg/ml) were observed against S. saprophyticus [stem (EtOAc, CHCl3 and hexane), leaves (MeOH, EtOH, EtOAc and CHCl3)], E. coli [stem (MeOH and EtOH), leaves (MeOH, EtOH, EtOAc and CHCl3)], M. catarrhalis [leaves (EtOAc and CHCl3)], K. pneumoniae [stem (CHCl3), leaves (CHCl3)] and S. aureus [leaves (CHCl3)]. Seven constituents [stigmasterol (1), stigmasta-4,22-dien-3-one (2), friedelin (3), friedelane-3-one-28-al (4), 3-O-acetyl-aleuritolic acid (5), 3-O-acetyl-erythrodiol (6) and methyl-3,4,5-trihydroxybenzoate (methyl gallate) (7)] were isolated from the stem MeOH extract. All these compounds displayed some antibacterial activity against the eight pathogens with highest activity against S. saprophyticus (2µg/ml). Furthermore, this is the first report of compounds 1, 2, 3, 4, 6 and 7 isolated from A. cordifolia and where a complete set of 2D-NMR data for fridelane-3-one-28-al (4) is presented. CONCLUSION: The study demonstrated that the antibacterial activities of A. cordifolia extracts may be due to the presence of the seven isolated compounds, where compounds 3-6 showed the best activity. The observed activity against gastrointestinal, skin, respiratory and urinary tract pathogens supports the traditional use for the treatment of such ailments. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26724423/Antibacterial_effects_of_Alchornea_cordifolia__Schumach__and_Thonn___Müll__Arg_extracts_and_compounds_on_gastrointestinal_skin_respiratory_and_urinary_tract_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30287-7 DB - PRIME DP - Unbound Medicine ER -