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Mutagenicity of antiviral substances of nucleobase analogue type in Salmonella typhimurium employing metabolic activation by mouse liver homogenate or cell-free plant extracts.
Zentralbl Mikrobiol 1989; 144(3):191-6ZM

Abstract

Five nucleobase analogues with antiviral properties were tested for their mutagenic activity in his mutant strains TA 1535, TA 1537, TA 1538, TA98, and TA 100 of S. typhimurium by means of preincubation tests with and without metabolic activation by cell free fractions from mouse liver (S-9) and maize seedlings (S-14). In one bacterial strain 6-azathymine increased the revertant counts in the absence of metabolic activation systems. In the presence of S-9 mix, the same substance became mutagenic for another tester strain. Metabolic activation by S-14 resulted in weak mutagenicity of 5-azadihydrouracil in high concentrations. 6-Azauracil, 5-azauracil, and 5-azadihydro-1,3-diacetyluracil were without mutagenic activity in all Salmonella-strains used. Cyclophosphamide, like other standard promutagens, was shown to become mutagenic in the presence of S-14 plant fraction. Thus S-14 activation system besides the S-9 liver system can be employed in mutagenicity testing with microbial systems.

Authors+Show Affiliations

Karl-Marx-Universität Leipzig, Wissenschaftsbereich Pflanzenphysiologie und Mikrobiologie, DDR.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2672681

Citation

el-Tarras, A, et al. "Mutagenicity of Antiviral Substances of Nucleobase Analogue Type in Salmonella Typhimurium Employing Metabolic Activation By Mouse Liver Homogenate or Cell-free Plant Extracts." Zentralblatt Fur Mikrobiologie, vol. 144, no. 3, 1989, pp. 191-6.
el-Tarras A, Braun R, Stenz E, et al. Mutagenicity of antiviral substances of nucleobase analogue type in Salmonella typhimurium employing metabolic activation by mouse liver homogenate or cell-free plant extracts. Zentralbl Mikrobiol. 1989;144(3):191-6.
el-Tarras, A., Braun, R., Stenz, E., & Schuster, G. (1989). Mutagenicity of antiviral substances of nucleobase analogue type in Salmonella typhimurium employing metabolic activation by mouse liver homogenate or cell-free plant extracts. Zentralblatt Fur Mikrobiologie, 144(3), pp. 191-6.
el-Tarras A, et al. Mutagenicity of Antiviral Substances of Nucleobase Analogue Type in Salmonella Typhimurium Employing Metabolic Activation By Mouse Liver Homogenate or Cell-free Plant Extracts. Zentralbl Mikrobiol. 1989;144(3):191-6. PubMed PMID: 2672681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity of antiviral substances of nucleobase analogue type in Salmonella typhimurium employing metabolic activation by mouse liver homogenate or cell-free plant extracts. AU - el-Tarras,A, AU - Braun,R, AU - Stenz,E, AU - Schuster,G, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 191 EP - 6 JF - Zentralblatt fur Mikrobiologie JO - Zentralbl. Mikrobiol. VL - 144 IS - 3 N2 - Five nucleobase analogues with antiviral properties were tested for their mutagenic activity in his mutant strains TA 1535, TA 1537, TA 1538, TA98, and TA 100 of S. typhimurium by means of preincubation tests with and without metabolic activation by cell free fractions from mouse liver (S-9) and maize seedlings (S-14). In one bacterial strain 6-azathymine increased the revertant counts in the absence of metabolic activation systems. In the presence of S-9 mix, the same substance became mutagenic for another tester strain. Metabolic activation by S-14 resulted in weak mutagenicity of 5-azadihydrouracil in high concentrations. 6-Azauracil, 5-azauracil, and 5-azadihydro-1,3-diacetyluracil were without mutagenic activity in all Salmonella-strains used. Cyclophosphamide, like other standard promutagens, was shown to become mutagenic in the presence of S-14 plant fraction. Thus S-14 activation system besides the S-9 liver system can be employed in mutagenicity testing with microbial systems. SN - 0232-4393 UR - https://www.unboundmedicine.com/medline/citation/2672681/Mutagenicity_of_antiviral_substances_of_nucleobase_analogue_type_in_Salmonella_typhimurium_employing_metabolic_activation_by_mouse_liver_homogenate_or_cell_free_plant_extracts_ DB - PRIME DP - Unbound Medicine ER -