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Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus.
PLoS One. 2016; 11(1):e0146312.Plos

Abstract

Lactobacillus rhamnosus GG (LGG) has been regarded as a safe probiotic strain. The aim of this study was to investigate whether dietary LGG supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by RV, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and LGG supplementing diet. On day 11, all pigs were orally infused RV or the sterile essential medium. RV infusion increased the diarrhea rate, increased the RV-Ab, NSP4 and IL-2 concentrations and the Bax mRNA levels of jejunal mucosa (P<0.05), decreased the villus height, villus height: crypt depth, the sIgA, IL-4 and mucin 1 concentrations and the ZO-1, occludin and Bcl-2 mRNA levels of jejunal mucosa (P<0.05), and affected the microbiota of ileum and cecum (P<0.05) in the weaned pigs. Dietary LGG supplementation increased the villus height and villus height: crypt depth, the sIgA, IL-4, mucin 1 and mucin 2 concentrations, and the ZO-1, occludin and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05) reduced the Bax mRNA levels of the jejunal mucosa (P<0.05) in weaned pigs. Furthermore, dietary LGG supplementation alleviated the increase of diarrhea rate in the weaned pigs challenged by RV (P<0.05), and relieve the effect of RV infection on the villus height, crypt depth and the villus height: crypt depth of the jejunal mucosa (P<0.05), the NSP4, sIgA, IL-2, IL-4, mucin 1 and mucin 2 concentrations of jejunal mucosa (P<0.05), the ZO-1, occludin, Bax and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05), and the microbiota of ileum and cecum (P<0.05) in the weaned pigs challenged by RV. These results suggest that supplementing LGG in diets alleviated the diarrhea of weaned piglets challenged by RV via inhibiting the virus multiplication and improving the jejunal mucosal barrier function, which was possibly due to the decreasing apoptosis of jejunal mucosal cells and the improvement of intestinal microbiota.

Authors+Show Affiliations

Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.Animal Nutrition Institute, Sichuan Agricultural University, Yucheng District, Ya'an, People's Republic of China. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, China, Ya'an, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26727003

Citation

Mao, Xiangbing, et al. "Dietary Lactobacillus Rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged By Porcine Rotavirus." PloS One, vol. 11, no. 1, 2016, pp. e0146312.
Mao X, Gu C, Hu H, et al. Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus. PLoS ONE. 2016;11(1):e0146312.
Mao, X., Gu, C., Hu, H., Tang, J., Chen, D., Yu, B., He, J., Yu, J., Luo, J., & Tian, G. (2016). Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus. PloS One, 11(1), e0146312. https://doi.org/10.1371/journal.pone.0146312
Mao X, et al. Dietary Lactobacillus Rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged By Porcine Rotavirus. PLoS ONE. 2016;11(1):e0146312. PubMed PMID: 26727003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus. AU - Mao,Xiangbing, AU - Gu,Changsong, AU - Hu,Haiyan, AU - Tang,Jun, AU - Chen,Daiwen, AU - Yu,Bing, AU - He,Jun, AU - Yu,Jie, AU - Luo,Junqiu, AU - Tian,Gang, Y1 - 2016/01/04/ PY - 2015/10/13/received PY - 2015/12/15/accepted PY - 2016/1/5/entrez PY - 2016/1/5/pubmed PY - 2016/7/7/medline SP - e0146312 EP - e0146312 JF - PloS one JO - PLoS ONE VL - 11 IS - 1 N2 - Lactobacillus rhamnosus GG (LGG) has been regarded as a safe probiotic strain. The aim of this study was to investigate whether dietary LGG supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by RV, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and LGG supplementing diet. On day 11, all pigs were orally infused RV or the sterile essential medium. RV infusion increased the diarrhea rate, increased the RV-Ab, NSP4 and IL-2 concentrations and the Bax mRNA levels of jejunal mucosa (P<0.05), decreased the villus height, villus height: crypt depth, the sIgA, IL-4 and mucin 1 concentrations and the ZO-1, occludin and Bcl-2 mRNA levels of jejunal mucosa (P<0.05), and affected the microbiota of ileum and cecum (P<0.05) in the weaned pigs. Dietary LGG supplementation increased the villus height and villus height: crypt depth, the sIgA, IL-4, mucin 1 and mucin 2 concentrations, and the ZO-1, occludin and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05) reduced the Bax mRNA levels of the jejunal mucosa (P<0.05) in weaned pigs. Furthermore, dietary LGG supplementation alleviated the increase of diarrhea rate in the weaned pigs challenged by RV (P<0.05), and relieve the effect of RV infection on the villus height, crypt depth and the villus height: crypt depth of the jejunal mucosa (P<0.05), the NSP4, sIgA, IL-2, IL-4, mucin 1 and mucin 2 concentrations of jejunal mucosa (P<0.05), the ZO-1, occludin, Bax and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05), and the microbiota of ileum and cecum (P<0.05) in the weaned pigs challenged by RV. These results suggest that supplementing LGG in diets alleviated the diarrhea of weaned piglets challenged by RV via inhibiting the virus multiplication and improving the jejunal mucosal barrier function, which was possibly due to the decreasing apoptosis of jejunal mucosal cells and the improvement of intestinal microbiota. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26727003/Dietary_Lactobacillus_rhamnosus_GG_Supplementation_Improves_the_Mucosal_Barrier_Function_in_the_Intestine_of_Weaned_Piglets_Challenged_by_Porcine_Rotavirus_ L2 - http://dx.plos.org/10.1371/journal.pone.0146312 DB - PRIME DP - Unbound Medicine ER -