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Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans.
J Agric Food Chem. 2016 Feb 10; 64(5):1144-50.JA

Abstract

Gluconobacter oxydans is used to produce xylitol from D-arabitol. This study aims to improve xylitol production by increasing the coenzyme regeneration efficiency of the pentose phosphate pathway in G. oxydans. Glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) were overexpressed in G. oxydans. Real-time PCR and enzyme activity assays revealed that G6PDH/6PGDH activity and coenzyme regeneration efficiency increased in the recombinant G. oxydans strains. Approximately 29.3 g/L xylitol was obtained, with a yield of 73.2%, from 40 g/L d-arabitol in the batch biotransformation with the G. oxydans PZ strain. Moreover, the xylitol productivity (0.62 g/L/h) was 3.26-fold of the wild type strain (0.19 g/L/h). In repetitive batch biotransformation, the G. oxydans PZ cells were used for five cycles without incurring a significant loss in productivity. These results indicate that the recombinant G. oxydans PZ strain is economically feasible for xylitol production in industrial bioconversion.

Authors+Show Affiliations

State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China. College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China. College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology , Nanjing 210009, People's Republic of China. College of Food Science and Light Industry, Nanjing University of Technology , Nanjing 211816, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26727541

Citation

Li, Sha, et al. "Improved Xylitol Production From D-Arabitol By Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter Oxydans." Journal of Agricultural and Food Chemistry, vol. 64, no. 5, 2016, pp. 1144-50.
Li S, Zhang J, Xu H, et al. Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans. J Agric Food Chem. 2016;64(5):1144-50.
Li, S., Zhang, J., Xu, H., & Feng, X. (2016). Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans. Journal of Agricultural and Food Chemistry, 64(5), 1144-50. https://doi.org/10.1021/acs.jafc.5b05509
Li S, et al. Improved Xylitol Production From D-Arabitol By Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter Oxydans. J Agric Food Chem. 2016 Feb 10;64(5):1144-50. PubMed PMID: 26727541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans. AU - Li,Sha, AU - Zhang,Jinliang, AU - Xu,Hong, AU - Feng,Xiaohai, Y1 - 2016/02/01/ PY - 2016/1/5/entrez PY - 2016/1/5/pubmed PY - 2016/10/8/medline KW - Gluconobacter oxydans KW - coenzyme regeneration KW - pentose phosphate pathway KW - xylitol SP - 1144 EP - 50 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 64 IS - 5 N2 - Gluconobacter oxydans is used to produce xylitol from D-arabitol. This study aims to improve xylitol production by increasing the coenzyme regeneration efficiency of the pentose phosphate pathway in G. oxydans. Glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) were overexpressed in G. oxydans. Real-time PCR and enzyme activity assays revealed that G6PDH/6PGDH activity and coenzyme regeneration efficiency increased in the recombinant G. oxydans strains. Approximately 29.3 g/L xylitol was obtained, with a yield of 73.2%, from 40 g/L d-arabitol in the batch biotransformation with the G. oxydans PZ strain. Moreover, the xylitol productivity (0.62 g/L/h) was 3.26-fold of the wild type strain (0.19 g/L/h). In repetitive batch biotransformation, the G. oxydans PZ cells were used for five cycles without incurring a significant loss in productivity. These results indicate that the recombinant G. oxydans PZ strain is economically feasible for xylitol production in industrial bioconversion. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/26727541/Improved_Xylitol_Production_from_D_Arabitol_by_Enhancing_the_Coenzyme_Regeneration_Efficiency_of_the_Pentose_Phosphate_Pathway_in_Gluconobacter_oxydans_ L2 - https://dx.doi.org/10.1021/acs.jafc.5b05509 DB - PRIME DP - Unbound Medicine ER -