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Antimicrobial properties of black grape (Vitis vinifera L.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds.
Indian J Pharmacol. 2015 Nov-Dec; 47(6):663-7.IJ

Abstract

AIM

Black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. The purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively.

MATERIALS AND METHODS

Peel of grape was subjected to polyphenolic extraction using different solvents viz., water, ethanol, acetone, and methanol. Antibiotic-resistant strains of Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella typhimurium, and Escherichia coli were screened for the antibacterial activity of different grape extracts. Antibacterial activity was analyzed using agar well diffusion method. Penicillium chrysogenum, Penicillium expansum, Aspergillus niger and Aspergillus versicolor were screened for the antifungal activity. Antifungal activity was determined by counting nongerminated spores in the presence of peel extracts.

RESULTS

As compared to other solvent extracts, methanol extracts possessed high antibacterial and antifungal activity. S. typhimurium and E. coli showed complete resistance against antibacterial action at screened concentrations of grape peel extracts. Maximum zone of inhibition was found in case of S. aureus, i.e., 22 mm followed by E. faecalis and E. aerogenes, i.e., 18 and 21 mm, respectively, at 1080 mg tannic acid equivalent (TAE)/ml. The maximum and minimum percent of growth inhibition was shown by P. expansum and A. niger as 73% and 15% at 1080 TAE/ml concentration of grape peel extract, respectively.

CONCLUSIONS

Except S. typhimurium and E. coli, growth of all bacterial and mold species were found to be significantly (P < 0.05) inhibited by all the solvent extracts.

Authors+Show Affiliations

Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana, India.Centre of Food Science and Technology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.Dairy Chemistry Division, National Dairy Research Institute, Karnal, Haryana, India.Dairy Technology Division, SRS, National Dairy Research Institute, Bengaluru, Karnataka, India.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26729960

Citation

Yadav, Devbrat, et al. "Antimicrobial Properties of Black Grape (Vitis Vinifera L.) Peel Extracts Against Antibiotic-resistant Pathogenic Bacteria and Toxin Producing Molds." Indian Journal of Pharmacology, vol. 47, no. 6, 2015, pp. 663-7.
Yadav D, Kumar A, Kumar P, et al. Antimicrobial properties of black grape (Vitis vinifera L.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds. Indian J Pharmacol. 2015;47(6):663-7.
Yadav, D., Kumar, A., Kumar, P., & Mishra, D. (2015). Antimicrobial properties of black grape (Vitis vinifera L.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds. Indian Journal of Pharmacology, 47(6), 663-7. https://doi.org/10.4103/0253-7613.169591
Yadav D, et al. Antimicrobial Properties of Black Grape (Vitis Vinifera L.) Peel Extracts Against Antibiotic-resistant Pathogenic Bacteria and Toxin Producing Molds. Indian J Pharmacol. 2015 Nov-Dec;47(6):663-7. PubMed PMID: 26729960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial properties of black grape (Vitis vinifera L.) peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds. AU - Yadav,Devbrat, AU - Kumar,Arvind, AU - Kumar,Pramod, AU - Mishra,Diwaker, PY - 2016/1/6/entrez PY - 2016/1/6/pubmed PY - 2016/10/14/medline KW - Antibacterial activity KW - Vitis vinifera L KW - antifungal activity KW - polyphenolic compounds KW - zone of inhibition SP - 663 EP - 7 JF - Indian journal of pharmacology JO - Indian J Pharmacol VL - 47 IS - 6 N2 - AIM: Black grape peel possesses a substantial amount of polyphenolic antimicrobial compounds that can be used for controlling the growth of pathogenic microorganisms. The purpose of this study was to assess antibacterial and antifungal activity of black grape peel extracts against antibiotic-resistant pathogenic bacteria and toxin producing molds, respectively. MATERIALS AND METHODS: Peel of grape was subjected to polyphenolic extraction using different solvents viz., water, ethanol, acetone, and methanol. Antibiotic-resistant strains of Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella typhimurium, and Escherichia coli were screened for the antibacterial activity of different grape extracts. Antibacterial activity was analyzed using agar well diffusion method. Penicillium chrysogenum, Penicillium expansum, Aspergillus niger and Aspergillus versicolor were screened for the antifungal activity. Antifungal activity was determined by counting nongerminated spores in the presence of peel extracts. RESULTS: As compared to other solvent extracts, methanol extracts possessed high antibacterial and antifungal activity. S. typhimurium and E. coli showed complete resistance against antibacterial action at screened concentrations of grape peel extracts. Maximum zone of inhibition was found in case of S. aureus, i.e., 22 mm followed by E. faecalis and E. aerogenes, i.e., 18 and 21 mm, respectively, at 1080 mg tannic acid equivalent (TAE)/ml. The maximum and minimum percent of growth inhibition was shown by P. expansum and A. niger as 73% and 15% at 1080 TAE/ml concentration of grape peel extract, respectively. CONCLUSIONS: Except S. typhimurium and E. coli, growth of all bacterial and mold species were found to be significantly (P < 0.05) inhibited by all the solvent extracts. SN - 1998-3751 UR - https://www.unboundmedicine.com/medline/citation/26729960/Antimicrobial_properties_of_black_grape__Vitis_vinifera_L___peel_extracts_against_antibiotic_resistant_pathogenic_bacteria_and_toxin_producing_molds_ L2 - http://www.ijp-online.com/article.asp?issn=0253-7613;year=2015;volume=47;issue=6;spage=663;epage=667;aulast=Yadav DB - PRIME DP - Unbound Medicine ER -