Tags

Type your tag names separated by a space and hit enter

Mechanism of ginsenoside Rg1 renal protection in a mouse model of d-galactose-induced subacute damage.
Pharm Biol. 2016 Sep; 54(9):1815-21.PB

Abstract

Context Ginseng is a widely used herbal medicine in China but its mechanism of action remains unclear. Objective The objectives of this work were to study the protective effect of ginsenoside Rg1 on subacute murine renal damage induced by d-galactose and its mechanism. Materials and methods C57BL/6J mice were injected with 120 mg/kg/d (sc) d-galactose for 1 week, followed by a combined treatment of Rg1 20 mg/kg/d (ip) and 120 mg/kg/d d-galactose (sc) for 5 weeks. Mice were injected with the 0.9% saline 0.2 mL/d (sc) and 120 mg/kg/d d-galactose (sc) for 6 weeks in the control group and the d-galactose group, respectively. After 6 weeks, urea, creatinine, uric acid, cystatin (Cys-C), senescence-associated β-galactosidase (SA-β-gal) staining positive kidney cells, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), glycation end products (AGEs) and 8-hydroxy-2 deoxyguanosine (8-OH-dG) were measured. Results Treatment with Rg1 ameliorated kidney function and aging state (urea from 17.19 ± 1.09 to 15.77 ± 1.22 mmol·L (-) (1), creatinine from 29.40 ± 5.72 to 22.60 ± 3.97 μmol·L (-) (1), uric acid from 86.80 ± 5.97 to 72.80 ± 10.61 μmol·L (-) (1), Cys-C from 0.23 ± 0.03 to 0.18 ± 0.05 mg·L (-) (1), ROD of SA-β-gal from 56.32 ± 10.48 to 26.78 ± 7.34, SOD from 150.22 ± 19.07 to 190.56 ± 15.83 U·(mg·prot) (-1), MDA from 9.28 ± 1.59 to 3.17 ± 0.82 nmol·(mg·prot) (-1), GSH-PX from 15.68 ± 2.11 to 20.32 ± 2.96 U·(mg·prot) (-1) as well as regulated glomerulus morphology (glomerulus diameter from 775.77 ± 18.41 to 695.04 ± 14.61 μm, renal capsule width from 39.56 ± 3.51 to 31.42 ± 2.70 μm, glomerulus basement membrane from 206.03 ± 16.22 to 157.27 ± 15.70 nm, podocyte slit from 55.21 ± 8.55 to 37.63 ± 6.65 nm). Conclusions Ginsenoside Rg1 can antagonise d-galactose subacute renal damage in mice and this may occur due to alleviating oxidative stress injury.

Authors+Show Affiliations

a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;b Chongqing Reproductive and Genetic Institute , Chongqing , China.a Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering , Chongqing Medical University , Chongqing , China ;

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26730750

Citation

Fan, Yanling, et al. "Mechanism of Ginsenoside Rg1 Renal Protection in a Mouse Model of D-galactose-induced Subacute Damage." Pharmaceutical Biology, vol. 54, no. 9, 2016, pp. 1815-21.
Fan Y, Xia J, Jia D, et al. Mechanism of ginsenoside Rg1 renal protection in a mouse model of d-galactose-induced subacute damage. Pharm Biol. 2016;54(9):1815-21.
Fan, Y., Xia, J., Jia, D., Zhang, M., Zhang, Y., Huang, G., & Wang, Y. (2016). Mechanism of ginsenoside Rg1 renal protection in a mouse model of d-galactose-induced subacute damage. Pharmaceutical Biology, 54(9), 1815-21. https://doi.org/10.3109/13880209.2015.1129543
Fan Y, et al. Mechanism of Ginsenoside Rg1 Renal Protection in a Mouse Model of D-galactose-induced Subacute Damage. Pharm Biol. 2016;54(9):1815-21. PubMed PMID: 26730750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of ginsenoside Rg1 renal protection in a mouse model of d-galactose-induced subacute damage. AU - Fan,Yanling, AU - Xia,Jieyu, AU - Jia,Daoyong, AU - Zhang,Mengsi, AU - Zhang,Yanyan, AU - Huang,Guoning, AU - Wang,Yaping, Y1 - 2016/01/05/ PY - 2016/1/6/entrez PY - 2016/1/6/pubmed PY - 2017/2/14/medline KW - Aging KW - Ginseng KW - kidney injury KW - protective mechanism SP - 1815 EP - 21 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 9 N2 - Context Ginseng is a widely used herbal medicine in China but its mechanism of action remains unclear. Objective The objectives of this work were to study the protective effect of ginsenoside Rg1 on subacute murine renal damage induced by d-galactose and its mechanism. Materials and methods C57BL/6J mice were injected with 120 mg/kg/d (sc) d-galactose for 1 week, followed by a combined treatment of Rg1 20 mg/kg/d (ip) and 120 mg/kg/d d-galactose (sc) for 5 weeks. Mice were injected with the 0.9% saline 0.2 mL/d (sc) and 120 mg/kg/d d-galactose (sc) for 6 weeks in the control group and the d-galactose group, respectively. After 6 weeks, urea, creatinine, uric acid, cystatin (Cys-C), senescence-associated β-galactosidase (SA-β-gal) staining positive kidney cells, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA), glycation end products (AGEs) and 8-hydroxy-2 deoxyguanosine (8-OH-dG) were measured. Results Treatment with Rg1 ameliorated kidney function and aging state (urea from 17.19 ± 1.09 to 15.77 ± 1.22 mmol·L (-) (1), creatinine from 29.40 ± 5.72 to 22.60 ± 3.97 μmol·L (-) (1), uric acid from 86.80 ± 5.97 to 72.80 ± 10.61 μmol·L (-) (1), Cys-C from 0.23 ± 0.03 to 0.18 ± 0.05 mg·L (-) (1), ROD of SA-β-gal from 56.32 ± 10.48 to 26.78 ± 7.34, SOD from 150.22 ± 19.07 to 190.56 ± 15.83 U·(mg·prot) (-1), MDA from 9.28 ± 1.59 to 3.17 ± 0.82 nmol·(mg·prot) (-1), GSH-PX from 15.68 ± 2.11 to 20.32 ± 2.96 U·(mg·prot) (-1) as well as regulated glomerulus morphology (glomerulus diameter from 775.77 ± 18.41 to 695.04 ± 14.61 μm, renal capsule width from 39.56 ± 3.51 to 31.42 ± 2.70 μm, glomerulus basement membrane from 206.03 ± 16.22 to 157.27 ± 15.70 nm, podocyte slit from 55.21 ± 8.55 to 37.63 ± 6.65 nm). Conclusions Ginsenoside Rg1 can antagonise d-galactose subacute renal damage in mice and this may occur due to alleviating oxidative stress injury. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/26730750/Mechanism_of_ginsenoside_Rg1_renal_protection_in_a_mouse_model_of_d_galactose_induced_subacute_damage_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2015.1129543 DB - PRIME DP - Unbound Medicine ER -