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In vitro and in vivo antitumour activities of puerarin 6″-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway.
Pharm Biol 2016; 54(9):1793-9PB

Abstract

Context Pueraria lobata (Leguminoseae) shows cytotoxic effects against cancer cells; however, its active components remain unclear. Objective This study investigated the antitumour activity of puerarin 6″-O-xyloside (POS) on the human lung carcinoma A549 cell line. Materials and methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxicity of POS (at 10, 20 and 40 μM) in vitro, and xenograft nude mice were established to evaluate the antitumour effect of POS (at 40 mg/kg/d) in vivo by 15 days intraperitoneal injection (ip). To explore its mechanism of action, flow cytometry was performed to determine the pro-apoptotic effect of POS (at 10, 20 and 40 μM). Subsequently, the expression of caspase-3, caspase-7, caspase-9, Bcl-2 and Bax in A549 cells were determined. Results POS showed significant cytotoxicity toward A549 cells (p < 0.05) by inducing apoptosis. Treatment with POS significantly upregulated the levels of caspase-3 (p < 0.01), caspase-7 (p < 0.01), caspase-9 (p < 0.01) and Bax (p < 0.01) in A549 cells, and Bcl-2 was downregulated (p < 0.01). Additionally, the in vivo animal study showed that POS significantly inhibited tumour growth in A549 cells (p < 0.01). Conclusion Our study demonstrated the POS has significant antitumour activities. The mechanisms are related to increased levels of caspase-3, caspase-7, caspase-9 and Bax, and reduced levels Bcl-2.

Authors+Show Affiliations

a Department of Oncology , Changshu No. 1 People's Hospital , Changshu , PR China.a Department of Oncology , Changshu No. 1 People's Hospital , Changshu , PR China.a Department of Oncology , Changshu No. 1 People's Hospital , Changshu , PR China.a Department of Oncology , Changshu No. 1 People's Hospital , Changshu , PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26730946

Citation

Chen, Ti, et al. "In Vitro and in Vivo Antitumour Activities of Puerarin 6″-O-xyloside On Human Lung Carcinoma A549 Cell Line Via the Induction of the Mitochondria-mediated Apoptosis Pathway." Pharmaceutical Biology, vol. 54, no. 9, 2016, pp. 1793-9.
Chen T, Chen H, Wang Y, et al. In vitro and in vivo antitumour activities of puerarin 6″-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway. Pharm Biol. 2016;54(9):1793-9.
Chen, T., Chen, H., Wang, Y., & Zhang, J. (2016). In vitro and in vivo antitumour activities of puerarin 6″-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway. Pharmaceutical Biology, 54(9), pp. 1793-9. doi:10.3109/13880209.2015.1127980.
Chen T, et al. In Vitro and in Vivo Antitumour Activities of Puerarin 6″-O-xyloside On Human Lung Carcinoma A549 Cell Line Via the Induction of the Mitochondria-mediated Apoptosis Pathway. Pharm Biol. 2016;54(9):1793-9. PubMed PMID: 26730946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo antitumour activities of puerarin 6″-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway. AU - Chen,Ti, AU - Chen,Hui, AU - Wang,Ying, AU - Zhang,Jian, Y1 - 2016/01/05/ PY - 2016/1/6/entrez PY - 2016/1/6/pubmed PY - 2017/2/14/medline KW - Bcl-2 KW - Pueraria lobata KW - caspase KW - xenograft model in nude mice SP - 1793 EP - 9 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 9 N2 - Context Pueraria lobata (Leguminoseae) shows cytotoxic effects against cancer cells; however, its active components remain unclear. Objective This study investigated the antitumour activity of puerarin 6″-O-xyloside (POS) on the human lung carcinoma A549 cell line. Materials and methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxicity of POS (at 10, 20 and 40 μM) in vitro, and xenograft nude mice were established to evaluate the antitumour effect of POS (at 40 mg/kg/d) in vivo by 15 days intraperitoneal injection (ip). To explore its mechanism of action, flow cytometry was performed to determine the pro-apoptotic effect of POS (at 10, 20 and 40 μM). Subsequently, the expression of caspase-3, caspase-7, caspase-9, Bcl-2 and Bax in A549 cells were determined. Results POS showed significant cytotoxicity toward A549 cells (p < 0.05) by inducing apoptosis. Treatment with POS significantly upregulated the levels of caspase-3 (p < 0.01), caspase-7 (p < 0.01), caspase-9 (p < 0.01) and Bax (p < 0.01) in A549 cells, and Bcl-2 was downregulated (p < 0.01). Additionally, the in vivo animal study showed that POS significantly inhibited tumour growth in A549 cells (p < 0.01). Conclusion Our study demonstrated the POS has significant antitumour activities. The mechanisms are related to increased levels of caspase-3, caspase-7, caspase-9 and Bax, and reduced levels Bcl-2. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/26730946/In_vitro_and_in_vivo_antitumour_activities_of_puerarin_6″_O_xyloside_on_human_lung_carcinoma_A549_cell_line_via_the_induction_of_the_mitochondria_mediated_apoptosis_pathway_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2015.1127980 DB - PRIME DP - Unbound Medicine ER -