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Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents.
Pharm Biol. 2016 Sep; 54(9):1822-30.PB

Abstract

Context Hamelia patens Jacq. (Rubiaceae) is traditionally used to treat wounds, inflammation and diabetes. However, there is still a lack of scientific evidence to support these applications. Objective The objective of this study is to evaluate the anti-inflammatory, antioxidant and antidiabetic activities of Hamelia patens, and identify its bioactive compounds. Materials and methods Four extracts were obtained by maceration and liquid-liquid extraction: HEX, DCM-EtOAc, MeOH-EtOAc and MeOH-Aq. The anti-inflammatory effect was evaluated orally on rat paw carrageenan-induced oedema over 6 h (50, 200 and 500 mg/kg), and topically in mouse ear oedema induced by 12-tetradecanoylphorbol-13-acetate (TPA) after 4 h (0.5 and 1 mg/ear). We also evaluated myeloperoxidase levels in ear tissue, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability, and in vitro α-glucosidase inhibition. The chemical compounds were separated by column chromatography and identified by spectroscopic analysis. Results We found that the oral administration of the HEX extract at 500 and 200 mg/kg significantly decreased the carrageenan-induced inflammation after 1 and 3 h, respectively. The MeOH-EtOAc extract significantly inhibited myeloperoxidase activity (83.5%), followed by the DCM-EtOAc extract (76%), β-sitosterol/stigmasterol (72.7%) and the HEX extract (55%), which significantly decreased oedema induced by TPA at both doses, giving a similar effect to indomethacin. We also found that the MeOH-EtOAc, MeOH-Aq and DCM-EtOAc extracts showed good DPPH scavenging activity (IC50 values of 18.6, 93.9 and 158.2 μg/mL, respectively). The HEX extract showed the lowest α-glucosidase inhibition (an IC50 value of 26.07 μg/mL), followed by the MeOH-EtOAc extract (an IC50 value of 30.18 μg/mL), β-sitosterol/stigmasterol (IC50 34.6 μg/mL) and compound A ((6E,10E,14E,18E)-2,6,10,14,18,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, an IC50 value of 114.6 μg/mL), which were isolated for the first time from Hamelia patens. Discussion and conclusion Hamelia patens possesses anti-inflammatory, antioxidant and α-glucosidase inhibitory activities, which support its traditional use. These effects can be attributed to the identified compounds.

Authors+Show Affiliations

a Unidad Académica Multidisciplinaria Zona Huasteca, Universidad Autónoma de San Luis Potosí , S.L.P , México ;b Instituto de Química, Universidad Nacional Autónoma de México , México D.F. , México.b Instituto de Química, Universidad Nacional Autónoma de México , México D.F. , México.a Unidad Académica Multidisciplinaria Zona Huasteca, Universidad Autónoma de San Luis Potosí , S.L.P , México ;

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26731099

Citation

Jiménez-Suárez, Verónica, et al. "Anti-inflammatory, Free Radical Scavenging and Alpha-glucosidase Inhibitory Activities of Hamelia Patens and Its Chemical Constituents." Pharmaceutical Biology, vol. 54, no. 9, 2016, pp. 1822-30.
Jiménez-Suárez V, Nieto-Camacho A, Jiménez-Estrada M, et al. Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents. Pharm Biol. 2016;54(9):1822-30.
Jiménez-Suárez, V., Nieto-Camacho, A., Jiménez-Estrada, M., & Alvarado Sánchez, B. (2016). Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents. Pharmaceutical Biology, 54(9), 1822-30. https://doi.org/10.3109/13880209.2015.1129544
Jiménez-Suárez V, et al. Anti-inflammatory, Free Radical Scavenging and Alpha-glucosidase Inhibitory Activities of Hamelia Patens and Its Chemical Constituents. Pharm Biol. 2016;54(9):1822-30. PubMed PMID: 26731099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents. AU - Jiménez-Suárez,Verónica, AU - Nieto-Camacho,Antonio, AU - Jiménez-Estrada,Manuel, AU - Alvarado Sánchez,Brenda, Y1 - 2016/01/05/ PY - 2016/1/6/entrez PY - 2016/1/6/pubmed PY - 2017/2/14/medline KW - 12-tetradecanoylphorbol-13-acetate KW - Antidiabetic KW - antioxidant KW - carrageenan KW - myeloperoxidase KW - squalene isomer KW - stigmasterol KW - β-sitosterol SP - 1822 EP - 30 JF - Pharmaceutical biology JO - Pharm Biol VL - 54 IS - 9 N2 - Context Hamelia patens Jacq. (Rubiaceae) is traditionally used to treat wounds, inflammation and diabetes. However, there is still a lack of scientific evidence to support these applications. Objective The objective of this study is to evaluate the anti-inflammatory, antioxidant and antidiabetic activities of Hamelia patens, and identify its bioactive compounds. Materials and methods Four extracts were obtained by maceration and liquid-liquid extraction: HEX, DCM-EtOAc, MeOH-EtOAc and MeOH-Aq. The anti-inflammatory effect was evaluated orally on rat paw carrageenan-induced oedema over 6 h (50, 200 and 500 mg/kg), and topically in mouse ear oedema induced by 12-tetradecanoylphorbol-13-acetate (TPA) after 4 h (0.5 and 1 mg/ear). We also evaluated myeloperoxidase levels in ear tissue, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability, and in vitro α-glucosidase inhibition. The chemical compounds were separated by column chromatography and identified by spectroscopic analysis. Results We found that the oral administration of the HEX extract at 500 and 200 mg/kg significantly decreased the carrageenan-induced inflammation after 1 and 3 h, respectively. The MeOH-EtOAc extract significantly inhibited myeloperoxidase activity (83.5%), followed by the DCM-EtOAc extract (76%), β-sitosterol/stigmasterol (72.7%) and the HEX extract (55%), which significantly decreased oedema induced by TPA at both doses, giving a similar effect to indomethacin. We also found that the MeOH-EtOAc, MeOH-Aq and DCM-EtOAc extracts showed good DPPH scavenging activity (IC50 values of 18.6, 93.9 and 158.2 μg/mL, respectively). The HEX extract showed the lowest α-glucosidase inhibition (an IC50 value of 26.07 μg/mL), followed by the MeOH-EtOAc extract (an IC50 value of 30.18 μg/mL), β-sitosterol/stigmasterol (IC50 34.6 μg/mL) and compound A ((6E,10E,14E,18E)-2,6,10,14,18,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, an IC50 value of 114.6 μg/mL), which were isolated for the first time from Hamelia patens. Discussion and conclusion Hamelia patens possesses anti-inflammatory, antioxidant and α-glucosidase inhibitory activities, which support its traditional use. These effects can be attributed to the identified compounds. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/26731099/Anti_inflammatory_free_radical_scavenging_and_alpha_glucosidase_inhibitory_activities_of_Hamelia_patens_and_its_chemical_constituents_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2015.1129544 DB - PRIME DP - Unbound Medicine ER -