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Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines.
PLoS One. 2016; 11(1):e0145928.Plos

Abstract

Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable traits that can be transferred to common wheat through breeding programs. The wheat-A. cristatum disomic addition and translocation lines can be used as bridge materials to introduce alien chromosomal segments to wheat. Wheat-A. cristatum 2P disomic addition line II-9-3 was highly resistant to powdery mildew and leaf rust, which was reported in our previous study. However, some translocation lines induced from II-9-3 have not been reported. In this study, some translocation lines were induced from II-9-3 by 60Co-γ irradiation and gametocidal chromosome 2C and then identified by cytological methods. Forty-nine wheat-A. cristatum translocation lines were obtained and various translcoation types were identified by GISH (genomic in situ hybridization), such as whole-arm, segmental and intercalary translocations. Dual-color FISH (fluorescent in situ hybridization) was applied to identify the wheat chromosomes involved in the translocations, and the results showed that A. cristatum 2P chromosome segments were translocated to the different wheat chromosomes, including 1A, 2A, 3A, 4A, 5A, 6A, 7A, 3B, 5B, 7B, 1D, 4D and 6D. Many different types of wheat-A. cristatum alien translocation lines would be valuable for not only identifying and cloning A. cristatum 2P-related genes and understanding the genetics and breeding effects of the translocation between A. cristatum chromosome 2P and wheat chromosomes, but also providing new germplasm resources for the wheat genetic improvement.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26731742

Citation

Li, Huanhuan, et al. "Production and Identification of Wheat-Agropyron Cristatum 2P Translocation Lines." PloS One, vol. 11, no. 1, 2016, pp. e0145928.
Li H, Lv M, Song L, et al. Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines. PLoS One. 2016;11(1):e0145928.
Li, H., Lv, M., Song, L., Zhang, J., Gao, A., Li, L., & Liu, W. (2016). Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines. PloS One, 11(1), e0145928. https://doi.org/10.1371/journal.pone.0145928
Li H, et al. Production and Identification of Wheat-Agropyron Cristatum 2P Translocation Lines. PLoS One. 2016;11(1):e0145928. PubMed PMID: 26731742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines. AU - Li,Huanhuan, AU - Lv,Mingjie, AU - Song,Liqiang, AU - Zhang,Jinpeng, AU - Gao,Ainong, AU - Li,Lihui, AU - Liu,Weihua, Y1 - 2016/01/05/ PY - 2015/08/27/received PY - 2015/12/10/accepted PY - 2016/1/6/entrez PY - 2016/1/6/pubmed PY - 2016/7/21/medline SP - e0145928 EP - e0145928 JF - PloS one JO - PLoS One VL - 11 IS - 1 N2 - Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable traits that can be transferred to common wheat through breeding programs. The wheat-A. cristatum disomic addition and translocation lines can be used as bridge materials to introduce alien chromosomal segments to wheat. Wheat-A. cristatum 2P disomic addition line II-9-3 was highly resistant to powdery mildew and leaf rust, which was reported in our previous study. However, some translocation lines induced from II-9-3 have not been reported. In this study, some translocation lines were induced from II-9-3 by 60Co-γ irradiation and gametocidal chromosome 2C and then identified by cytological methods. Forty-nine wheat-A. cristatum translocation lines were obtained and various translcoation types were identified by GISH (genomic in situ hybridization), such as whole-arm, segmental and intercalary translocations. Dual-color FISH (fluorescent in situ hybridization) was applied to identify the wheat chromosomes involved in the translocations, and the results showed that A. cristatum 2P chromosome segments were translocated to the different wheat chromosomes, including 1A, 2A, 3A, 4A, 5A, 6A, 7A, 3B, 5B, 7B, 1D, 4D and 6D. Many different types of wheat-A. cristatum alien translocation lines would be valuable for not only identifying and cloning A. cristatum 2P-related genes and understanding the genetics and breeding effects of the translocation between A. cristatum chromosome 2P and wheat chromosomes, but also providing new germplasm resources for the wheat genetic improvement. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26731742/Production_and_Identification_of_Wheat_Agropyron_cristatum_2P_Translocation_Lines_ L2 - https://dx.plos.org/10.1371/journal.pone.0145928 DB - PRIME DP - Unbound Medicine ER -