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Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp.
PLoS Negl Trop Dis. 2016 Jan; 10(1):e0004309.PN

Abstract

Paracoccidioides spp., a dimorphic pathogenic fungus, is the etiologic agent of paracoccidioidomycosis (PCM). PCM is an endemic disease that affects at least 10 million people in Latin America, causing severe public health problems. The drugs used against pathogenic fungi have various side effects and limited efficacy; therefore, there is an inevitable and urgent medical need for the development of new antifungal drugs. In the present study, we evaluated the transcriptional profile of Paracoccidioides lutzii exposed to argentilactone, a constituent of the essential oil of Hyptis ovalifolia. A total of 1,058 genes were identified, of which 208 were up-regulated and 850 were down-regulated. Cell rescue, defense and virulence, with a total of 26 genes, was a functional category with a large number of genes induced, including heat shock protein 90 (hsp90), cytochrome c peroxidase (ccp), the hemoglobin ligand RBT5 (rbt5) and superoxide dismutase (sod). Quantitative real-time PCR revealed an increase in the expression level of all of those genes. An enzymatic assay showed a significant increase in SOD activity. The reduced growth of Pbhsp90-aRNA, Pbccp-aRNA, Pbsod-aRNA and Pbrbt5-aRNA isolates in the presence of argentilactone indicates the importance of these genes in the response of Paracoccidioides spp. to argentilactone. The response of the P. lutzii cell wall to argentilactone treatment was also evaluated. The results showed that argentilactone caused a decrease in the levels of polymers in the cell wall. These results suggest that argentilactone is a potential candidate for antifungal therapy.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Produtos Naturais, Instituto de Química, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biodados, Biologia Celular e Desenvolvimento, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil.Unidad de Biología Celular y Molecular, Corporación para Investigaciones Biológicas (CIB) and Escuela de Microbiología Universidad de Antioquia, Medellín, Colombia.Unidad de Biología Celular y Molecular, Corporación para Investigaciones Biológicas (CIB) and Facultad de Medicina Universidad de Antioquia, Medellín, Colombia.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26734764

Citation

Araújo, Felipe Souto, et al. "Effects of Argentilactone On the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides Spp." PLoS Neglected Tropical Diseases, vol. 10, no. 1, 2016, pp. e0004309.
Araújo FS, Coelho LM, Silva Ldo C, et al. Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp. PLoS Negl Trop Dis. 2016;10(1):e0004309.
Araújo, F. S., Coelho, L. M., Silva, L. d. o. . C., da Silva Neto, B. R., Parente-Rocha, J. A., Bailão, A. M., de Oliveira, C. M., Fernandes, G. d. a. . R., Hernández, O., Ochoa, J. G., Soares, C. M., & Pereira, M. (2016). Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp. PLoS Neglected Tropical Diseases, 10(1), e0004309. https://doi.org/10.1371/journal.pntd.0004309
Araújo FS, et al. Effects of Argentilactone On the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides Spp. PLoS Negl Trop Dis. 2016;10(1):e0004309. PubMed PMID: 26734764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Argentilactone on the Transcriptional Profile, Cell Wall and Oxidative Stress of Paracoccidioides spp. AU - Araújo,Felipe Souto, AU - Coelho,Luciene Melo, AU - Silva,Lívia do Carmo, AU - da Silva Neto,Benedito Rodrigues, AU - Parente-Rocha,Juliana Alves, AU - Bailão,Alexandre Melo, AU - de Oliveira,Cecília Maria Alves, AU - Fernandes,Gabriel da Rocha, AU - Hernández,Orville, AU - Ochoa,Juan Guillermo McEwen, AU - Soares,Célia Maria de Almeida, AU - Pereira,Maristela, Y1 - 2016/01/06/ PY - 2015/06/15/received PY - 2015/11/26/accepted PY - 2016/1/7/entrez PY - 2016/1/7/pubmed PY - 2016/5/3/medline SP - e0004309 EP - e0004309 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 10 IS - 1 N2 - Paracoccidioides spp., a dimorphic pathogenic fungus, is the etiologic agent of paracoccidioidomycosis (PCM). PCM is an endemic disease that affects at least 10 million people in Latin America, causing severe public health problems. The drugs used against pathogenic fungi have various side effects and limited efficacy; therefore, there is an inevitable and urgent medical need for the development of new antifungal drugs. In the present study, we evaluated the transcriptional profile of Paracoccidioides lutzii exposed to argentilactone, a constituent of the essential oil of Hyptis ovalifolia. A total of 1,058 genes were identified, of which 208 were up-regulated and 850 were down-regulated. Cell rescue, defense and virulence, with a total of 26 genes, was a functional category with a large number of genes induced, including heat shock protein 90 (hsp90), cytochrome c peroxidase (ccp), the hemoglobin ligand RBT5 (rbt5) and superoxide dismutase (sod). Quantitative real-time PCR revealed an increase in the expression level of all of those genes. An enzymatic assay showed a significant increase in SOD activity. The reduced growth of Pbhsp90-aRNA, Pbccp-aRNA, Pbsod-aRNA and Pbrbt5-aRNA isolates in the presence of argentilactone indicates the importance of these genes in the response of Paracoccidioides spp. to argentilactone. The response of the P. lutzii cell wall to argentilactone treatment was also evaluated. The results showed that argentilactone caused a decrease in the levels of polymers in the cell wall. These results suggest that argentilactone is a potential candidate for antifungal therapy. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/26734764/Effects_of_Argentilactone_on_the_Transcriptional_Profile_Cell_Wall_and_Oxidative_Stress_of_Paracoccidioides_spp_ L2 - http://dx.plos.org/10.1371/journal.pntd.0004309 DB - PRIME DP - Unbound Medicine ER -