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Repeated exposures to roadside particulate matter extracts suppresses pulmonary defense mechanisms, resulting in lipid and protein oxidative damage.
Environ Pollut. 2016 Mar; 210:227-37.EP

Abstract

Exposure to particulate matter (PM) pollution in cities and urban canyons can be harmful to the exposed population. However, the underlying mechanisms that lead to health effects are not yet elucidated. It is postulated that exposure to repeated, small, environmentally relevant concentrations can affect lung homeostasis. This study examines the impact of repeated exposures to urban PM on mouse lungs with focus on inflammatory and oxidative stress parameters. Aqueous extracts from collected urban PM were administered to mice by 5 repeated intra-tracheal instillations (IT). Multiple exposures, led to an increase in cytokine levels in both bronchoalveolar lavage fluid and in the blood serum, indicating a systemic reaction. Lung mRNA levels of antioxidant/phase II detoxifying enzymes decreased by exposure to the PM extract, but not when metals were removed by chelation. Finally, disruption of lung tissue oxidant-inflammatory/defense balance was evidenced by increased levels of lipid and protein oxidation. Unlike response to a single IT exposure to the same dose and source of extract, multiple exposures result in lung oxidative damage and a systemic inflammatory reaction. These could be attributed to compromised capacity to activate the protective Nrf2 tissue defense system. It is suggested that water-soluble metals present in urban PM, potentially from break and tire wear, may constitute major drivers of the pulmonary and systemic responses to multiple exposure to urban PM.

Authors+Show Affiliations

Department of Earth and Planetary Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel.Flow Cytometry Unit, Weizmann Institute of Science, Rehovot 76100, Israel.Department of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, and the National Institute of Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, Israel.Environmental Chemistry and Technology Program, University of Wisconsin-Madison, Madison, WI, USA.Department of Earth and Planetary Sciences, Weizmann Institute of Science, Rehovot, 76100, Israel. Electronic address: yinon.rudich@weizmann.ac.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26735168

Citation

Pardo, Michal, et al. "Repeated Exposures to Roadside Particulate Matter Extracts Suppresses Pulmonary Defense Mechanisms, Resulting in Lipid and Protein Oxidative Damage." Environmental Pollution (Barking, Essex : 1987), vol. 210, 2016, pp. 227-37.
Pardo M, Porat Z, Rudich A, et al. Repeated exposures to roadside particulate matter extracts suppresses pulmonary defense mechanisms, resulting in lipid and protein oxidative damage. Environ Pollut. 2016;210:227-37.
Pardo, M., Porat, Z., Rudich, A., Schauer, J. J., & Rudich, Y. (2016). Repeated exposures to roadside particulate matter extracts suppresses pulmonary defense mechanisms, resulting in lipid and protein oxidative damage. Environmental Pollution (Barking, Essex : 1987), 210, 227-37. https://doi.org/10.1016/j.envpol.2015.12.009
Pardo M, et al. Repeated Exposures to Roadside Particulate Matter Extracts Suppresses Pulmonary Defense Mechanisms, Resulting in Lipid and Protein Oxidative Damage. Environ Pollut. 2016;210:227-37. PubMed PMID: 26735168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Repeated exposures to roadside particulate matter extracts suppresses pulmonary defense mechanisms, resulting in lipid and protein oxidative damage. AU - Pardo,Michal, AU - Porat,Ziv, AU - Rudich,Assaf, AU - Schauer,James J, AU - Rudich,Yinon, Y1 - 2015/12/29/ PY - 2015/08/27/received PY - 2015/12/02/revised PY - 2015/12/05/accepted PY - 2016/1/7/entrez PY - 2016/1/7/pubmed PY - 2016/12/15/medline KW - Air pollution KW - Inflammation KW - Intra-tracheal installation KW - Nrf2 KW - Oxidative stress KW - Roadway metals SP - 227 EP - 37 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 210 N2 - Exposure to particulate matter (PM) pollution in cities and urban canyons can be harmful to the exposed population. However, the underlying mechanisms that lead to health effects are not yet elucidated. It is postulated that exposure to repeated, small, environmentally relevant concentrations can affect lung homeostasis. This study examines the impact of repeated exposures to urban PM on mouse lungs with focus on inflammatory and oxidative stress parameters. Aqueous extracts from collected urban PM were administered to mice by 5 repeated intra-tracheal instillations (IT). Multiple exposures, led to an increase in cytokine levels in both bronchoalveolar lavage fluid and in the blood serum, indicating a systemic reaction. Lung mRNA levels of antioxidant/phase II detoxifying enzymes decreased by exposure to the PM extract, but not when metals were removed by chelation. Finally, disruption of lung tissue oxidant-inflammatory/defense balance was evidenced by increased levels of lipid and protein oxidation. Unlike response to a single IT exposure to the same dose and source of extract, multiple exposures result in lung oxidative damage and a systemic inflammatory reaction. These could be attributed to compromised capacity to activate the protective Nrf2 tissue defense system. It is suggested that water-soluble metals present in urban PM, potentially from break and tire wear, may constitute major drivers of the pulmonary and systemic responses to multiple exposure to urban PM. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/26735168/Repeated_exposures_to_roadside_particulate_matter_extracts_suppresses_pulmonary_defense_mechanisms_resulting_in_lipid_and_protein_oxidative_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(15)30221-9 DB - PRIME DP - Unbound Medicine ER -