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Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test.
Drug Chem Toxicol. 2016; 39(3):312-21.DC

Abstract

CONTEXT

Celtis glabrata is used in Turkey for the treatment of various health disorders.

OBJECTIVE

The acetone, chloroform, ethanol, and methanol extracts of C. glabrata leaf, fruit, and seed were investigated to evaluate their antimutagenic activities.

MATERIAL AND METHODS

The antimutagenicity of these extracts was determined by Ames test against mutagens (4-nitro-O-phenylenediamine, 2-aminofluorene (2-AF), and sodium azide (SA)). The extracts were used at concentrations between 5 and 0.005 mg/plate.

RESULTS

The ethanol extracts of leaves exhibited strong antimutagenicity (70%) against 2-AF with S9 at 5 mg/plate on TA98. But methanol (61%, 53%) and acetone (53%, 52%) also revealed strong inhibition rates at concentrations of ≥ 0.5 mg/plate. Among the extracts, the highest activity (96%) was obtained from acetone extract against SA without S9, followed by chloroform extract (91%) at a dose of 5 mg/plate on TA100 with S9. Ethanol (without S9) and chloroform (with S9) extracts showed strong antimutagenicity at all doses. Exception of chloroform and acetone (without S9), all fruit extracts (with/without S9) manifested strong antimutagenicity at doses of ≥ 0.5 mg/plate on TA98 strain. Ethanol extracts revealed 68% inhibition against 2-AF on TA98. Acetone and ethanol extracts manifested 84% and 82% inhibition against SA on TA100, respectively. All the extracts of seeds revealed strong inhibition against 2-AF at ≥ 0.5 mg/plate doses on TA98, but acetone extract showed excellent antimutagenicity (94%). Moreover, the chloroform (74, 73, 63, 54%), acetone (74, 72, 70, 65%) and methanol (74, 67, 63, 61%) extracts of seeds revealed strong antimutagenic activity on TA100 against SA with S9.

DISCUSSION AND CONCLUSION

This plant may be natural source of antimutagenic agents.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Akin D
a Meram Faculty of Medicine, Department of Anatomy , Necmettin Erbakan University , Konya , Turkey .
Durak Y
b Faculty of Science, Department of Biology , Selcuk University , Konya , Turkey , and.
Uysal A
c Vocational School of Health Services, Department of Medicinal Laboratory , Selcuk University , Konya , Turkey.
Gunes E
b Faculty of Science, Department of Biology , Selcuk University , Konya , Turkey , and.
Aladag MO
c Vocational School of Health Services, Department of Medicinal Laboratory , Selcuk University , Konya , Turkey.

MeSH

Antimutagenic AgentsBase Pair MismatchCannabaceaeFrameshift MutationFruitMutagensPlant ExtractsPlant LeavesSalmonella typhimuriumSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26739110

Citation

Akin, Duygu, et al. "Assessment of Antimutagenic Action of Celtis Glabrata Steven Ex Planch. (Cannabaceae) Extracts Against Base Pair Exchange and Frame Shift Mutations On Salmonella Typhimurium TA98 and TA100 Strains By Ames Test." Drug and Chemical Toxicology, vol. 39, no. 3, 2016, pp. 312-21.
Akin D, Durak Y, Uysal A, et al. Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test. Drug Chem Toxicol. 2016;39(3):312-21.
Akin, D., Durak, Y., Uysal, A., Gunes, E., & Aladag, M. O. (2016). Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test. Drug and Chemical Toxicology, 39(3), 312-21. https://doi.org/10.3109/01480545.2015.1121273
Akin D, et al. Assessment of Antimutagenic Action of Celtis Glabrata Steven Ex Planch. (Cannabaceae) Extracts Against Base Pair Exchange and Frame Shift Mutations On Salmonella Typhimurium TA98 and TA100 Strains By Ames Test. Drug Chem Toxicol. 2016;39(3):312-21. PubMed PMID: 26739110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test. AU - Akin,Duygu, AU - Durak,Yusuf, AU - Uysal,Ahmet, AU - Gunes,Erdogan, AU - Aladag,Mustafa Onur, Y1 - 2016/01/07/ PY - 2016/1/8/entrez PY - 2016/1/8/pubmed PY - 2016/12/31/medline KW - Cannabaceae KW - Salmonella/microsome test system KW - antimutagenicity KW - free radical scavenging KW - total phenol content SP - 312 EP - 21 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 39 IS - 3 N2 - CONTEXT: Celtis glabrata is used in Turkey for the treatment of various health disorders. OBJECTIVE: The acetone, chloroform, ethanol, and methanol extracts of C. glabrata leaf, fruit, and seed were investigated to evaluate their antimutagenic activities. MATERIAL AND METHODS: The antimutagenicity of these extracts was determined by Ames test against mutagens (4-nitro-O-phenylenediamine, 2-aminofluorene (2-AF), and sodium azide (SA)). The extracts were used at concentrations between 5 and 0.005 mg/plate. RESULTS: The ethanol extracts of leaves exhibited strong antimutagenicity (70%) against 2-AF with S9 at 5 mg/plate on TA98. But methanol (61%, 53%) and acetone (53%, 52%) also revealed strong inhibition rates at concentrations of ≥ 0.5 mg/plate. Among the extracts, the highest activity (96%) was obtained from acetone extract against SA without S9, followed by chloroform extract (91%) at a dose of 5 mg/plate on TA100 with S9. Ethanol (without S9) and chloroform (with S9) extracts showed strong antimutagenicity at all doses. Exception of chloroform and acetone (without S9), all fruit extracts (with/without S9) manifested strong antimutagenicity at doses of ≥ 0.5 mg/plate on TA98 strain. Ethanol extracts revealed 68% inhibition against 2-AF on TA98. Acetone and ethanol extracts manifested 84% and 82% inhibition against SA on TA100, respectively. All the extracts of seeds revealed strong inhibition against 2-AF at ≥ 0.5 mg/plate doses on TA98, but acetone extract showed excellent antimutagenicity (94%). Moreover, the chloroform (74, 73, 63, 54%), acetone (74, 72, 70, 65%) and methanol (74, 67, 63, 61%) extracts of seeds revealed strong antimutagenic activity on TA100 against SA with S9. DISCUSSION AND CONCLUSION: This plant may be natural source of antimutagenic agents. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/26739110/Assessment_of_antimutagenic_action_of_Celtis_glabrata_Steven_ex_Planch___Cannabaceae__extracts_against_base_pair_exchange_and_frame_shift_mutations_on_Salmonella_typhimurium_TA98_and_TA100_strains_by_Ames_test_ DB - PRIME DP - Unbound Medicine ER -