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Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus).
Biol Open 2016; 5(1):62-71BO

Abstract

Understanding the mechanisms that protect against or limit muscle atrophy in hibernators during prolonged inactivity has important implications for its treatment. We examined whether external factors influence the pathways regulating protein synthesis and degradation, leading to muscle atrophy prevention in Daurian ground squirrels (Spermophilus dauricus). We investigated the effects of 14-day hindlimb-unloading (HU) in different seasons and two-month hibernation on the soleus (SOL) muscle wet mass, muscle-to-body mass ratio, fiber cross sectional area (CSA), fiber distribution and muscle ultrastructure. We also measured changes in the protein expression and activation states of Akt, mTOR and FoxO1 and the mRNA expression of atrogin-1 and MuRF1. Compared with the control groups, autumn and winter HU significantly lowered SOL muscle wet mass and muscle-to-body mass ratio, decreased type I and II fiber CSA and induced ultrastructural anomalies. However, these measured indices were unchanged between Pre-hibernation and Hibernation groups. Furthermore, phosphorylation levels of Akt and mTOR significantly decreased, while the phosphorylation level of FoxO1 and mRNA expression of atrogin-1 and MuRF1 increased after HU. During hibernation, the phosphorylation levels of Akt and mTOR significantly decreased, but the phosphorylation level of FoxO1 and mRNA expression of atrogin-1 and MuRF1 remained unchanged. Overall, our findings suggest that disuse and seasonality may not be sufficient to initiate the innate protective mechanism that prevents SOL atrophy during prolonged periods of hibernation inactivity. The stable expression of atrogin-1 and MuRF1 may facilitate to prevent SOL atrophy via controlling ubiquitination of muscle proteins during hibernation.

Authors+Show Affiliations

Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China.Gravitational Physiology and Medicine Research Unit, Institute of Physiology, Center of Physiological Medicine, Medical University Graz, Graz 8010, Austria gaoyunf@nwu.edu.cn nandu.goswami@medunigraz.at.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China gaoyunf@nwu.edu.cn nandu.goswami@medunigraz.at.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26740574

Citation

Dang, Kai, et al. "Stable Atrogin-1 (Fbxo32) and MuRF1 (Trim63) Gene Expression Is Involved in the Protective Mechanism in Soleus Muscle of Hibernating Daurian Ground Squirrels (Spermophilus Dauricus)." Biology Open, vol. 5, no. 1, 2016, pp. 62-71.
Dang K, Li YZ, Gong LC, et al. Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). Biol Open. 2016;5(1):62-71.
Dang, K., Li, Y. Z., Gong, L. C., Xue, W., Wang, H. P., Goswami, N., & Gao, Y. F. (2016). Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). Biology Open, 5(1), pp. 62-71. doi:10.1242/bio.015776.
Dang K, et al. Stable Atrogin-1 (Fbxo32) and MuRF1 (Trim63) Gene Expression Is Involved in the Protective Mechanism in Soleus Muscle of Hibernating Daurian Ground Squirrels (Spermophilus Dauricus). Biol Open. 2016 Jan 6;5(1):62-71. PubMed PMID: 26740574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (Spermophilus dauricus). AU - Dang,Kai, AU - Li,Ya-Zhao, AU - Gong,Ling-Chen, AU - Xue,Wei, AU - Wang,Hui-Ping, AU - Goswami,Nandu, AU - Gao,Yun-Fang, Y1 - 2016/01/06/ PY - 2016/1/8/entrez PY - 2016/1/8/pubmed PY - 2016/1/8/medline KW - Disuse muscle atrophy KW - Hibernation KW - Hindlimb-unloading KW - Seasons SP - 62 EP - 71 JF - Biology open JO - Biol Open VL - 5 IS - 1 N2 - Understanding the mechanisms that protect against or limit muscle atrophy in hibernators during prolonged inactivity has important implications for its treatment. We examined whether external factors influence the pathways regulating protein synthesis and degradation, leading to muscle atrophy prevention in Daurian ground squirrels (Spermophilus dauricus). We investigated the effects of 14-day hindlimb-unloading (HU) in different seasons and two-month hibernation on the soleus (SOL) muscle wet mass, muscle-to-body mass ratio, fiber cross sectional area (CSA), fiber distribution and muscle ultrastructure. We also measured changes in the protein expression and activation states of Akt, mTOR and FoxO1 and the mRNA expression of atrogin-1 and MuRF1. Compared with the control groups, autumn and winter HU significantly lowered SOL muscle wet mass and muscle-to-body mass ratio, decreased type I and II fiber CSA and induced ultrastructural anomalies. However, these measured indices were unchanged between Pre-hibernation and Hibernation groups. Furthermore, phosphorylation levels of Akt and mTOR significantly decreased, while the phosphorylation level of FoxO1 and mRNA expression of atrogin-1 and MuRF1 increased after HU. During hibernation, the phosphorylation levels of Akt and mTOR significantly decreased, but the phosphorylation level of FoxO1 and mRNA expression of atrogin-1 and MuRF1 remained unchanged. Overall, our findings suggest that disuse and seasonality may not be sufficient to initiate the innate protective mechanism that prevents SOL atrophy during prolonged periods of hibernation inactivity. The stable expression of atrogin-1 and MuRF1 may facilitate to prevent SOL atrophy via controlling ubiquitination of muscle proteins during hibernation. SN - 2046-6390 UR - https://www.unboundmedicine.com/medline/citation/26740574/Stable_atrogin_1__Fbxo32__and_MuRF1__Trim63__gene_expression_is_involved_in_the_protective_mechanism_in_soleus_muscle_of_hibernating_Daurian_ground_squirrels__Spermophilus_dauricus__ L2 - http://bio.biologists.org/cgi/pmidlookup?view=long&pmid=26740574 DB - PRIME DP - Unbound Medicine ER -