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Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience.
Asian Pac J Cancer Prev. 2015; 16(18):8495-501.AP

Abstract

PURPOSE

This study was conducted to assess the agreement and differences between cervical self-sampling with a Kato device (KSSD) and gynecologist sampling for Pap cytology and human papillomavirus DNA (HPV DNA) detection.

MATERIALS AND METHODS

Women underwent self-sampling followed by gynecologist sampling during screening at two primary health clinics. Pap cytology of cervical specimens was evaluated for specimen adequacy, presence of endocervical cells or transformation zone cells and cytological interpretation for cells abnormalities. Cervical specimens were also extracted and tested for HPV DNA detection. Positive HPV smears underwent gene sequencing and HPV genotyping by referring to the online NCBI gene bank. Results were compared between samplings by Kappa agreement and McNemar test.

RESULTS

For Pap specimen adequacy, KSSD showed 100% agreement with gynecologist sampling but had only 32.3% agreement for presence of endocervical cells. Both sampling showed 100% agreement with only 1 case detected HSIL favouring CIN2 for cytology result. HPV DNA detection showed 86.2%agreement (K=0.64, 95% CI 0.524-0.756, p=0.001) between samplings. KSSD and gynaecologist sampling identified high risk HPV in 17.3% and 23.9% respectively (p= 0.014).

CONCLUSION

The self-sampling using Kato device can serve as a tool in Pap cytology and HPV DNA detection in low resource settings in Malaysia. Self-sampling devices such as KSSD can be used as an alternative technique to gynaecologist sampling for cervical cancer screening among rural populations in Malaysia.

Authors+Show Affiliations

Cancer Resource and Education Center (CaRE), Universiti Putra Malaysia, Malaysia E-mail : latiffah.latiff@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

26745108

Citation

Latiff, Latiffah A., et al. "Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 16, no. 18, 2015, pp. 8495-501.
Latiff LA, Ibrahim Z, Pei CP, et al. Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience. Asian Pac J Cancer Prev. 2015;16(18):8495-501.
Latiff, L. A., Ibrahim, Z., Pei, C. P., Rahman, S. A., & Akhtari-Zavare, M. (2015). Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience. Asian Pacific Journal of Cancer Prevention : APJCP, 16(18), 8495-501.
Latiff LA, et al. Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience. Asian Pac J Cancer Prev. 2015;16(18):8495-501. PubMed PMID: 26745108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Assessment of a Self-sampling Device and Gynecologist Sampling for Cytology and HPV DNA Detection in a Rural and Low Resource Setting: Malaysian Experience. AU - Latiff,Latiffah A, AU - Ibrahim,Zaidah, AU - Pei,Chong Pei, AU - Rahman,Sabariah Abdul, AU - Akhtari-Zavare,Mehrnoosh, PY - 2016/1/9/entrez PY - 2016/1/9/pubmed PY - 2016/10/7/medline SP - 8495 EP - 501 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac. J. Cancer Prev. VL - 16 IS - 18 N2 - PURPOSE: This study was conducted to assess the agreement and differences between cervical self-sampling with a Kato device (KSSD) and gynecologist sampling for Pap cytology and human papillomavirus DNA (HPV DNA) detection. MATERIALS AND METHODS: Women underwent self-sampling followed by gynecologist sampling during screening at two primary health clinics. Pap cytology of cervical specimens was evaluated for specimen adequacy, presence of endocervical cells or transformation zone cells and cytological interpretation for cells abnormalities. Cervical specimens were also extracted and tested for HPV DNA detection. Positive HPV smears underwent gene sequencing and HPV genotyping by referring to the online NCBI gene bank. Results were compared between samplings by Kappa agreement and McNemar test. RESULTS: For Pap specimen adequacy, KSSD showed 100% agreement with gynecologist sampling but had only 32.3% agreement for presence of endocervical cells. Both sampling showed 100% agreement with only 1 case detected HSIL favouring CIN2 for cytology result. HPV DNA detection showed 86.2%agreement (K=0.64, 95% CI 0.524-0.756, p=0.001) between samplings. KSSD and gynaecologist sampling identified high risk HPV in 17.3% and 23.9% respectively (p= 0.014). CONCLUSION: The self-sampling using Kato device can serve as a tool in Pap cytology and HPV DNA detection in low resource settings in Malaysia. Self-sampling devices such as KSSD can be used as an alternative technique to gynaecologist sampling for cervical cancer screening among rural populations in Malaysia. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/26745108/Comparative_Assessment_of_a_Self_sampling_Device_and_Gynecologist_Sampling_for_Cytology_and_HPV_DNA_Detection_in_a_Rural_and_Low_Resource_Setting:_Malaysian_Experience_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:26745108&key=2015.16.18.8495 DB - PRIME DP - Unbound Medicine ER -