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Genetics and mapping of a novel downy mildew resistance gene, Pl(18), introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.).
Theor Appl Genet. 2016 Apr; 129(4):741-752.TA

Abstract

KEY MESSAGE

A novel downy mildew resistance gene, Pl(18), was introgressed from wild Helianthus argophyllus into cultivated sunflower and genetically mapped to linkage group 2 of the sunflower genome. The new germplasm, HA-DM1, carrying Pl(18) has been released to the public. Sunflower downy mildew (DM) is considered to be the most destructive foliar disease that has spread to every major sunflower-growing country of the world, except Australia. A new dominant downy mildew resistance gene (Pl 18) transferred from wild Helianthus argophyllus (PI 494573) into cultivated sunflower was mapped to linkage group (LG) 2 of the sunflower genome using bulked segregant analysis with 869 simple sequence repeat (SSR) markers. Phenotyping 142 BC1F2:3 families derived from the cross of HA 89 and H. argophyllus confirmed the single gene inheritance of resistance. Since no other Pl gene has been mapped to LG2, this gene was novel and designated as Pl (18). SSR markers CRT214 and ORS203 flanked Pl(18) at a genetic distance of 1.1 and 0.4 cM, respectively. Forty-six single nucleotide polymorphism (SNP) markers that cover the Pl(18) region were surveyed for saturation mapping of the region. Six co-segregating SNP markers were 1.2 cM distal to Pl(18), and another four co-segregating SNP markers were 0.9 cM proximal to Pl(18). The new BC2F4-derived germplasm, HA-DM1, carrying Pl(18) has been released to the public. This new line is highly resistant to all Plasmopara halstedii races identified in the USA providing breeders with an effective new source of resistance against downy mildew in sunflower. The molecular markers that were developed will be especially useful in marker-assisted selection and pyramiding of Pl resistance genes because of their close proximity to the gene and the availability of high-throughput SNP detection assays.

Authors+Show Affiliations

USDA-Agricultural Research Service, Northern Crop Science Laboratory, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA. lili.qi@ars.usda.gov.USDA-Agricultural Research Service, Northern Crop Science Laboratory, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA.Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.USDA-Agricultural Research Service, Northern Crop Science Laboratory, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26747047

Citation

Qi, L L., et al. "Genetics and Mapping of a Novel Downy Mildew Resistance Gene, Pl(18), Introgressed From Wild Helianthus Argophyllus Into Cultivated Sunflower (Helianthus Annuus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 4, 2016, pp. 741-752.
Qi LL, Foley ME, Cai XW, et al. Genetics and mapping of a novel downy mildew resistance gene, Pl(18), introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.). Theor Appl Genet. 2016;129(4):741-752.
Qi, L. L., Foley, M. E., Cai, X. W., & Gulya, T. J. (2016). Genetics and mapping of a novel downy mildew resistance gene, Pl(18), introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(4), 741-752. https://doi.org/10.1007/s00122-015-2662-2
Qi LL, et al. Genetics and Mapping of a Novel Downy Mildew Resistance Gene, Pl(18), Introgressed From Wild Helianthus Argophyllus Into Cultivated Sunflower (Helianthus Annuus L.). Theor Appl Genet. 2016;129(4):741-752. PubMed PMID: 26747047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetics and mapping of a novel downy mildew resistance gene, Pl(18), introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.). AU - Qi,L L, AU - Foley,M E, AU - Cai,X W, AU - Gulya,T J, Y1 - 2016/01/08/ PY - 2015/10/06/received PY - 2015/12/14/accepted PY - 2016/1/10/entrez PY - 2016/1/10/pubmed PY - 2016/7/23/medline SP - 741 EP - 752 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 129 IS - 4 N2 - KEY MESSAGE: A novel downy mildew resistance gene, Pl(18), was introgressed from wild Helianthus argophyllus into cultivated sunflower and genetically mapped to linkage group 2 of the sunflower genome. The new germplasm, HA-DM1, carrying Pl(18) has been released to the public. Sunflower downy mildew (DM) is considered to be the most destructive foliar disease that has spread to every major sunflower-growing country of the world, except Australia. A new dominant downy mildew resistance gene (Pl 18) transferred from wild Helianthus argophyllus (PI 494573) into cultivated sunflower was mapped to linkage group (LG) 2 of the sunflower genome using bulked segregant analysis with 869 simple sequence repeat (SSR) markers. Phenotyping 142 BC1F2:3 families derived from the cross of HA 89 and H. argophyllus confirmed the single gene inheritance of resistance. Since no other Pl gene has been mapped to LG2, this gene was novel and designated as Pl (18). SSR markers CRT214 and ORS203 flanked Pl(18) at a genetic distance of 1.1 and 0.4 cM, respectively. Forty-six single nucleotide polymorphism (SNP) markers that cover the Pl(18) region were surveyed for saturation mapping of the region. Six co-segregating SNP markers were 1.2 cM distal to Pl(18), and another four co-segregating SNP markers were 0.9 cM proximal to Pl(18). The new BC2F4-derived germplasm, HA-DM1, carrying Pl(18) has been released to the public. This new line is highly resistant to all Plasmopara halstedii races identified in the USA providing breeders with an effective new source of resistance against downy mildew in sunflower. The molecular markers that were developed will be especially useful in marker-assisted selection and pyramiding of Pl resistance genes because of their close proximity to the gene and the availability of high-throughput SNP detection assays. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26747047/Genetics_and_mapping_of_a_novel_downy_mildew_resistance_gene_Pl_18__introgressed_from_wild_Helianthus_argophyllus_into_cultivated_sunflower__Helianthus_annuus_L___ L2 - https://dx.doi.org/10.1007/s00122-015-2662-2 DB - PRIME DP - Unbound Medicine ER -