Type your tag names separated by a space and hit enter

Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics.

Abstract

Consumption of a high fat meal can increase neutrophilic airway inflammation in asthma subjects. This study investigates the molecular mechanisms driving airway neutrophilia following a high fat meal in asthmatics. Subjects with asthma (n = 11) and healthy controls (n = 8) consumed a high-fat/energy meal, containing total energy (TE) of 3846 kJ and 48 g of total fat (20.5 g saturated). Sputum was induced at 0 and 4 h, and gene expression was examined by microarray and quantitative real-time PCR (qPCR). Following the high fat dietary challenge, 168 entities were significantly differentially expressed greater than >1.5 fold in subjects with asthma, whereas, in healthy controls, only 14 entities were differentially expressed. Of the 168 genes that were changed in asthma, several biological processes were overrepresented, with 25 genes involved in "immune system processes". qPCR confirmed that S100P, S100A16, MAL and MUC1 were significantly increased in the asthma group post-meal. We also observed a strong correlation and a moderate correlation between the change in NLRP12 and S100A16 gene expression at 4 h compared to baseline, and the change in total and saturated non-esterified plasma fatty acid levels at 2 h compared to baseline. In summary, our data identifies differences in inflammatory gene expression that may contribute to increased airway neutrophilia following a high fat meal in subjects with asthma and may provide useful therapeutic targets for immunomodulation. This may be particularly relevant to obese asthmatics, who are habitually consuming diets with a high fat content.

Links

  • PMC Free PDF
  • PMC Free Full Text
  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, University of Newcastle, NSW 2308, Australia. qli3@uon.edu.au.

    ,

    Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, University of Newcastle, NSW 2308, Australia. katherine.baines@newcastle.edu.au.

    ,

    Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, University of Newcastle, NSW 2308, Australia. Peter.gibson@hnehealth.nsw.gov.au. Department of Respiratory and Sleep Medicine, John Hunter Hospital, Newcastle, NSW 2305, Australia. Peter.gibson@hnehealth.nsw.gov.au.

    Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, University of Newcastle, NSW 2308, Australia. lisa.wood@newcastle.edu.au.

    Source

    Nutrients 8:1 2016 Jan 07 pg

    MeSH

    Adult
    Airway Obstruction
    Asthma
    Calcium-Binding Proteins
    Case-Control Studies
    Diet, High-Fat
    Dietary Fats
    Fatty Acids, Nonesterified
    Female
    Gene Expression
    Humans
    Inflammation
    Male
    Meals
    Middle Aged
    Mucin-1
    Myelin and Lymphocyte-Associated Proteolipid Proteins
    Neoplasm Proteins
    Neutrophils
    Postprandial Period
    Real-Time Polymerase Chain Reaction
    S100 Proteins
    Sputum
    Time Factors

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26751474

    Citation

    Li, Qian, et al. "Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics." Nutrients, vol. 8, no. 1, 2016.
    Li Q, Baines KJ, Gibson PG, et al. Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics. Nutrients. 2016;8(1).
    Li, Q., Baines, K. J., Gibson, P. G., & Wood, L. G. (2016). Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics. Nutrients, 8(1), doi:10.3390/nu8010030.
    Li Q, et al. Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics. Nutrients. 2016 Jan 7;8(1) PubMed PMID: 26751474.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Changes in Expression of Genes Regulating Airway Inflammation Following a High-Fat Mixed Meal in Asthmatics. AU - Li,Qian, AU - Baines,Katherine J, AU - Gibson,Peter G, AU - Wood,Lisa G, Y1 - 2016/01/07/ PY - 2015/11/13/received PY - 2015/12/10/revised PY - 2015/12/22/accepted PY - 2016/1/12/entrez PY - 2016/1/12/pubmed PY - 2016/9/30/medline KW - asthma KW - fatty acids KW - inflammation JF - Nutrients JO - Nutrients VL - 8 IS - 1 N2 - Consumption of a high fat meal can increase neutrophilic airway inflammation in asthma subjects. This study investigates the molecular mechanisms driving airway neutrophilia following a high fat meal in asthmatics. Subjects with asthma (n = 11) and healthy controls (n = 8) consumed a high-fat/energy meal, containing total energy (TE) of 3846 kJ and 48 g of total fat (20.5 g saturated). Sputum was induced at 0 and 4 h, and gene expression was examined by microarray and quantitative real-time PCR (qPCR). Following the high fat dietary challenge, 168 entities were significantly differentially expressed greater than >1.5 fold in subjects with asthma, whereas, in healthy controls, only 14 entities were differentially expressed. Of the 168 genes that were changed in asthma, several biological processes were overrepresented, with 25 genes involved in "immune system processes". qPCR confirmed that S100P, S100A16, MAL and MUC1 were significantly increased in the asthma group post-meal. We also observed a strong correlation and a moderate correlation between the change in NLRP12 and S100A16 gene expression at 4 h compared to baseline, and the change in total and saturated non-esterified plasma fatty acid levels at 2 h compared to baseline. In summary, our data identifies differences in inflammatory gene expression that may contribute to increased airway neutrophilia following a high fat meal in subjects with asthma and may provide useful therapeutic targets for immunomodulation. This may be particularly relevant to obese asthmatics, who are habitually consuming diets with a high fat content. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/26751474/full_citation L2 - http://www.mdpi.com/resolver?pii=nu8010030 DB - PRIME DP - Unbound Medicine ER -