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(GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication.
Comp Cytogenet. 2015; 9(4):533-47.CC

Abstract

Although the wheat A genomes have been intensively studied over past decades, many questions concerning the mechanisms of their divergence and evolution still remain unsolved. In the present study we performed comparative analysis of the A genome chromosomes in diploid (Triticum urartu Tumanian ex Gandilyan, 1972, Triticum boeoticum Boissier, 1874 and Triticum monococcum Linnaeus, 1753) and polyploid wheat species representing two evolutionary lineages, Timopheevi (Triticum timopheevii (Zhukovsky) Zhukovsky, 1934 and Triticum zhukovskyi Menabde & Ericzjan, 1960) and Emmer (Triticum dicoccoides (Körnicke ex Ascherson & Graebner) Schweinfurth, 1908, Triticum durum Desfontaines, 1798, and Triticum aestivum Linnaeus, 1753) using a new cytogenetic marker - the pTm30 probe cloned from Triticum monococcum genome and containing (GAA)56 microsatellite sequence. Up to four pTm30 sites located on 1AS, 5AS, 2AS, and 4AL chromosomes have been revealed in the wild diploid species, although most accessions contained one-two (GAA)n sites. The domesticated diploid species Triticum monococcum differs from the wild diploid species by almost complete lack of polymorphism in the distribution of (GAA)n site. Only one (GAA)n site in the 4AL chromosome has been found in Triticum monococcum. Among three wild emmer (Triticum dicoccoides) accessions we detected 4 conserved and 9 polymorphic (GAA)n sites in the A genome. The (GAA)n loci on chromosomes 2AS, 4AL, and 5AL found in of Triticum dicoccoides were retained in Triticum durum and Triticum aestivum. In species of the Timopheevi lineage, the only one, large (GAA)n site has been detected in the short arm of 6A(t) chromosome. (GAA)n site observed in Triticum monococcum are undetectable in the A(b) genome of Triticum zhukovskyi, this site could be eliminated over the course of amphiploidization, while the species was established. We also demonstrated that changes in the distribution of (GAA)n sequence on the A-genome chromosomes of diploid and polyploid wheats are associated with chromosomal rearrangements/ modifications, involving mainly the NOR (nucleolus organizer region)-bearing chromosomes, that took place during the evolution of wild and domesticated species.

Authors+Show Affiliations

Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090 Russia.Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090 Russia.N.I.Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina street 3, Moscow 119991, Russia.Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090 Russia.Siberian Research Institute of Plant Growing and Selection - Branch of ICG SB RAS, Krasnoobsk, Novosibirsk Region, Russia.Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090 Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26753073

Citation

Adonina, Irina G., et al. "(GAA)n Microsatellite as an Indicator of the a Genome Reorganization During Wheat Evolution and Domestication." Comparative Cytogenetics, vol. 9, no. 4, 2015, pp. 533-47.
Adonina IG, Goncharov NP, Badaeva ED, et al. (GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication. Comp Cytogenet. 2015;9(4):533-47.
Adonina, I. G., Goncharov, N. P., Badaeva, E. D., Sergeeva, E. M., Petrash, N. V., & Salina, E. A. (2015). (GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication. Comparative Cytogenetics, 9(4), 533-47. https://doi.org/10.3897/CompCytogen.v9i4.5120
Adonina IG, et al. (GAA)n Microsatellite as an Indicator of the a Genome Reorganization During Wheat Evolution and Domestication. Comp Cytogenet. 2015;9(4):533-47. PubMed PMID: 26753073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - (GAA)n microsatellite as an indicator of the A genome reorganization during wheat evolution and domestication. AU - Adonina,Irina G, AU - Goncharov,Nikolay P, AU - Badaeva,Ekaterina D, AU - Sergeeva,Ekaterina M, AU - Petrash,Nadezhda V, AU - Salina,Elena A, Y1 - 2015/09/02/ PY - 2015/04/16/received PY - 2014/07/15/accepted PY - 2016/1/12/entrez PY - 2016/1/12/pubmed PY - 2016/1/12/medline KW - (GAA)n microsatellite KW - FISH KW - Triticum boeoticum KW - Triticum dicoccoides KW - Triticum monococcum KW - Triticum urartu KW - Triticum zhukovskyi SP - 533 EP - 47 JF - Comparative cytogenetics JO - Comp Cytogenet VL - 9 IS - 4 N2 - Although the wheat A genomes have been intensively studied over past decades, many questions concerning the mechanisms of their divergence and evolution still remain unsolved. In the present study we performed comparative analysis of the A genome chromosomes in diploid (Triticum urartu Tumanian ex Gandilyan, 1972, Triticum boeoticum Boissier, 1874 and Triticum monococcum Linnaeus, 1753) and polyploid wheat species representing two evolutionary lineages, Timopheevi (Triticum timopheevii (Zhukovsky) Zhukovsky, 1934 and Triticum zhukovskyi Menabde & Ericzjan, 1960) and Emmer (Triticum dicoccoides (Körnicke ex Ascherson & Graebner) Schweinfurth, 1908, Triticum durum Desfontaines, 1798, and Triticum aestivum Linnaeus, 1753) using a new cytogenetic marker - the pTm30 probe cloned from Triticum monococcum genome and containing (GAA)56 microsatellite sequence. Up to four pTm30 sites located on 1AS, 5AS, 2AS, and 4AL chromosomes have been revealed in the wild diploid species, although most accessions contained one-two (GAA)n sites. The domesticated diploid species Triticum monococcum differs from the wild diploid species by almost complete lack of polymorphism in the distribution of (GAA)n site. Only one (GAA)n site in the 4AL chromosome has been found in Triticum monococcum. Among three wild emmer (Triticum dicoccoides) accessions we detected 4 conserved and 9 polymorphic (GAA)n sites in the A genome. The (GAA)n loci on chromosomes 2AS, 4AL, and 5AL found in of Triticum dicoccoides were retained in Triticum durum and Triticum aestivum. In species of the Timopheevi lineage, the only one, large (GAA)n site has been detected in the short arm of 6A(t) chromosome. (GAA)n site observed in Triticum monococcum are undetectable in the A(b) genome of Triticum zhukovskyi, this site could be eliminated over the course of amphiploidization, while the species was established. We also demonstrated that changes in the distribution of (GAA)n sequence on the A-genome chromosomes of diploid and polyploid wheats are associated with chromosomal rearrangements/ modifications, involving mainly the NOR (nucleolus organizer region)-bearing chromosomes, that took place during the evolution of wild and domesticated species. SN - 1993-0771 UR - https://www.unboundmedicine.com/medline/citation/26753073/_GAA_n_microsatellite_as_an_indicator_of_the_A_genome_reorganization_during_wheat_evolution_and_domestication_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26753073/ DB - PRIME DP - Unbound Medicine ER -
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