TY - JOUR T1 - Analytical Enantioseparation of β-Substituted-2-Phenylpropionic Acids by High-Performance Liquid Chromatography with Hydroxypropyl-β-Cyclodextrin as Chiral Mobile Phase Additive. AU - Tong,Shengqiang, AU - Zhang,Hu, AU - Yan,Jizhong, Y1 - 2016/01/10/ PY - 2015/04/26/received PY - 2015/12/01/accepted PY - 2016/1/13/entrez PY - 2016/1/13/pubmed PY - 2016/11/15/medline SP - 593 EP - 7 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 54 IS - 4 N2 - Analytical enantioseparation of five β-substituted-2-phenylpropionic acids by high-performance liquid chromatography with hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral mobile phase additive was established in this paper, and chromatographic retention mechanism was studied. The effects of various factors such as the organic modifier, different ODS C18 columns and concentration of HP-β-CD were investigated. The chiral mobile phase was composed of methanol or acetonitrile and 0.5% triethylamine acetate buffer at pH 3.0 added with 25 mmol L(-1) of HP-β-CD, and baseline separations could be reached for all racemates. As for chromatographic retention mechanism, it was found that there was a negative correlation between the concentration of HP-β-CD in mobile phase and the retention factor under constant pH value and column temperature. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/26755500/Analytical_Enantioseparation_of_β_Substituted_2_Phenylpropionic_Acids_by_High_Performance_Liquid_Chromatography_with_Hydroxypropyl_β_Cyclodextrin_as_Chiral_Mobile_Phase_Additive_ DB - PRIME DP - Unbound Medicine ER -