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Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles.
Dis Aquat Organ. 2016 Jan 13; 117(3):205-14.DA

Abstract

Engineered nanoparticles (NPs) can potentially generate adverse effects at the tissue, organ, cellular, subcellular, DNA, and protein levels due to their unique physico-chemical properties. Dissoluble NPs (e.g. nZnO) can be toxic in aquatic organisms. We compared effects of nZnO and corresponding concentrations of released Zn(II) by water-soluble ZnCl(2) on larval zebrafish Danio rerio (72 h post fertilization) by analyzing changes in expression levels of stress-related genes (p53, rad51, mt2) by qRT-PCR. Additionally, genotoxicity of nZnO and Zn(II) was assessed. The lethal concentrations for 50% mortality (LC(50)) in larval zebrafish exposed for 96 h to 0 to 70 mg l(-1) nZnO and Zn(II) were 21.37 ± 1.81 mg l(-1) (95% CI) and 4.66 ± 0.11 mg l(-1), respectively. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed (96 h) to nZnO and Zn(II). DNA damage was higher in Zn(II)- than nZnO-exposed larvae. Induction of stress-related genes in larvae was complex and was not directly related to nZnO and Zn(II) concentrations, although there was significant induction in the mt2 gene of larvae exposed to Zn(II) and nZnO relative to controls. mt2 induction of 20.5 ± 1.9-fold and 2.5 ± 0.8-fold change (mean ± SEM) was observed in larvae at the highest Zn(II) and nZnO concentrations (3 and 6 mg l(-1)), respectively. The results suggest that toxicity associated with nZnO is primarily due to the release of Zn(II).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Boran H
Recep Tayyip Erdoğan University, Faculty of Fisheries, 53100 Rize, Turkey.
Ulutas G
No affiliation info available

MeSH

AnimalsChloridesDNA DamageEmbryo, NonmammalianMetal NanoparticlesMutagenicity TestsStress, PhysiologicalZebrafishZinc CompoundsZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26758654

Citation

Boran, Halis, and Gokay Ulutas. "Genotoxic Effects and Gene Expression Changes in Larval Zebrafish After Exposure to ZnCl2 and ZnO Nanoparticles." Diseases of Aquatic Organisms, vol. 117, no. 3, 2016, pp. 205-14.
Boran H, Ulutas G. Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles. Dis Aquat Organ. 2016;117(3):205-14.
Boran, H., & Ulutas, G. (2016). Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles. Diseases of Aquatic Organisms, 117(3), 205-14. https://doi.org/10.3354/dao02943
Boran H, Ulutas G. Genotoxic Effects and Gene Expression Changes in Larval Zebrafish After Exposure to ZnCl2 and ZnO Nanoparticles. Dis Aquat Organ. 2016 Jan 13;117(3):205-14. PubMed PMID: 26758654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic effects and gene expression changes in larval zebrafish after exposure to ZnCl2 and ZnO nanoparticles. AU - Boran,Halis, AU - Ulutas,Gokay, PY - 2016/1/14/entrez PY - 2016/1/14/pubmed PY - 2016/4/22/medline SP - 205 EP - 14 JF - Diseases of aquatic organisms JO - Dis Aquat Organ VL - 117 IS - 3 N2 - Engineered nanoparticles (NPs) can potentially generate adverse effects at the tissue, organ, cellular, subcellular, DNA, and protein levels due to their unique physico-chemical properties. Dissoluble NPs (e.g. nZnO) can be toxic in aquatic organisms. We compared effects of nZnO and corresponding concentrations of released Zn(II) by water-soluble ZnCl(2) on larval zebrafish Danio rerio (72 h post fertilization) by analyzing changes in expression levels of stress-related genes (p53, rad51, mt2) by qRT-PCR. Additionally, genotoxicity of nZnO and Zn(II) was assessed. The lethal concentrations for 50% mortality (LC(50)) in larval zebrafish exposed for 96 h to 0 to 70 mg l(-1) nZnO and Zn(II) were 21.37 ± 1.81 mg l(-1) (95% CI) and 4.66 ± 0.11 mg l(-1), respectively. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed (96 h) to nZnO and Zn(II). DNA damage was higher in Zn(II)- than nZnO-exposed larvae. Induction of stress-related genes in larvae was complex and was not directly related to nZnO and Zn(II) concentrations, although there was significant induction in the mt2 gene of larvae exposed to Zn(II) and nZnO relative to controls. mt2 induction of 20.5 ± 1.9-fold and 2.5 ± 0.8-fold change (mean ± SEM) was observed in larvae at the highest Zn(II) and nZnO concentrations (3 and 6 mg l(-1)), respectively. The results suggest that toxicity associated with nZnO is primarily due to the release of Zn(II). SN - 0177-5103 UR - https://www.unboundmedicine.com/medline/citation/26758654/Genotoxic_effects_and_gene_expression_changes_in_larval_zebrafish_after_exposure_to_ZnCl2_and_ZnO_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -