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Application of diffusion-edited and solvent suppression ¹H-NMR to the direct analysis of markers in valerian-hop liquid herbal products.
Phytochem Anal. 2016 Mar-Apr; 27(2):100-6.PA

Abstract

INTRODUCTION

The rising trend to consume herbal products for the treatment and/or prevention of minor ailments together with their chemical and pharmacological complexity means there is an urgent need to develop new approaches to their quality and stability.

OBJECTIVES

This work looks at the application of one-dimensional diffusion-edited (1)H-NMR spectroscopy (1D DOSY) and (1)H-NMR with suppression of the ethanol and water signals to the characterisation of quality and stability markers in multi-component herbal medicines/food supplements.

MATERIAL AND METHODS

The experiments were performed with commercial tinctures of Valeriana officinalis L. (valerian), expired and non-expired, as well as its combination with Hummulus lupulus L. (hops), which is one of the most popular blends of relaxant herbs. These techniques did not require purification or evaporation of components for the qualitative analysis of the mixture, but only the addition of D2 O and TSP.

RESULTS

The best diagnostic signals were found at δ 7 ppm (H-11, valerenic acid), δ 4.2 ppm (H-1, hydroxyvalerenic acid) and δ 1.5-1.8 ppm (methyl groups in prenylated moieties, α-acids/prenylated flavones).

CONCLUSION

This work concludes on the potential value of 1D DOSY (1)H-NMR to provide additional assurance of quality in complex natural mixtures.

Authors+Show Affiliations

Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX, UK.Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX, UK.Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX, UK. Department of Pharmacy, University of Hertfordshire, College Lane, Hatfield, AL10 9AB, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26763752

Citation

Prieto, Jose M., et al. "Application of Diffusion-edited and Solvent Suppression ¹H-NMR to the Direct Analysis of Markers in Valerian-hop Liquid Herbal Products." Phytochemical Analysis : PCA, vol. 27, no. 2, 2016, pp. 100-6.
Prieto JM, Mellinas-Gomez M, Zloh M. Application of diffusion-edited and solvent suppression ¹H-NMR to the direct analysis of markers in valerian-hop liquid herbal products. Phytochem Anal. 2016;27(2):100-6.
Prieto, J. M., Mellinas-Gomez, M., & Zloh, M. (2016). Application of diffusion-edited and solvent suppression ¹H-NMR to the direct analysis of markers in valerian-hop liquid herbal products. Phytochemical Analysis : PCA, 27(2), 100-6. https://doi.org/10.1002/pca.2603
Prieto JM, Mellinas-Gomez M, Zloh M. Application of Diffusion-edited and Solvent Suppression ¹H-NMR to the Direct Analysis of Markers in Valerian-hop Liquid Herbal Products. Phytochem Anal. 2016 Mar-Apr;27(2):100-6. PubMed PMID: 26763752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of diffusion-edited and solvent suppression ¹H-NMR to the direct analysis of markers in valerian-hop liquid herbal products. AU - Prieto,Jose M, AU - Mellinas-Gomez,Maria, AU - Zloh,Mire, Y1 - 2016/01/13/ PY - 2015/03/14/received PY - 2015/10/11/revised PY - 2015/10/14/accepted PY - 2016/1/15/entrez PY - 2016/1/15/pubmed PY - 2016/11/1/medline KW - Herbal medicinal product KW - Humulus lupulus L KW - Valeriana officinalis KW - diffusion edited spectroscopy KW - nuclear magnetic resonance KW - quality marker SP - 100 EP - 6 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 27 IS - 2 N2 - INTRODUCTION: The rising trend to consume herbal products for the treatment and/or prevention of minor ailments together with their chemical and pharmacological complexity means there is an urgent need to develop new approaches to their quality and stability. OBJECTIVES: This work looks at the application of one-dimensional diffusion-edited (1)H-NMR spectroscopy (1D DOSY) and (1)H-NMR with suppression of the ethanol and water signals to the characterisation of quality and stability markers in multi-component herbal medicines/food supplements. MATERIAL AND METHODS: The experiments were performed with commercial tinctures of Valeriana officinalis L. (valerian), expired and non-expired, as well as its combination with Hummulus lupulus L. (hops), which is one of the most popular blends of relaxant herbs. These techniques did not require purification or evaporation of components for the qualitative analysis of the mixture, but only the addition of D2 O and TSP. RESULTS: The best diagnostic signals were found at δ 7 ppm (H-11, valerenic acid), δ 4.2 ppm (H-1, hydroxyvalerenic acid) and δ 1.5-1.8 ppm (methyl groups in prenylated moieties, α-acids/prenylated flavones). CONCLUSION: This work concludes on the potential value of 1D DOSY (1)H-NMR to provide additional assurance of quality in complex natural mixtures. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/26763752/Application_of_diffusion_edited_and_solvent_suppression_¹H_NMR_to_the_direct_analysis_of_markers_in_valerian_hop_liquid_herbal_products_ L2 - https://doi.org/10.1002/pca.2603 DB - PRIME DP - Unbound Medicine ER -
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