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Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major.
Fish Shellfish Immunol. 2016 Feb; 49:275-85.FS

Abstract

Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P < 0.05). Serum lysozyme activity (LZY) significantly increased in LR + LL when compared with the control group. Additionally, fish fed the LR + LL diet showed a higher growth performance (Fn wt, WG, and SGR) and protein digestibility than the groups fed an individual LR or the control diet. Superoxide dismutase (SOD) significantly increased in LR and LR + LL groups when compared with the other groups. Moreover, the fish fed LR or LL had better improvement (P < 0.05) in growth, feed utilization, body protein and lipid contents, digestibility coefficients (dry matter, protein, and lipid), protease activity, total intestine and LAB counts, hematocrit, total plasma protein, biological antioxidant potential, ACP, serum and mucus LZY and bactericidal activities, peroxidase, SOD, and mucus secretion than the control group. Interestingly, fish fed diets with LR + LL showed significantly lower total cholesterol and triglycerides when compared with the other groups (P < 0.05). These data strongly suggest that a mixture of LR and LL probiotics may serve as a healthy immunostimulating feed additive in red sea bream aquaculture.

Authors+Show Affiliations

The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, Japan; Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan; Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt. Electronic address: mahmouddawood55@gmail.com.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan; Department of Animal Production, Faculty of Agriculture, Tanta University, 31527, Tanta, Egypt.The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, Japan; Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, Japan; Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.The United Graduate School of Agriculture Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0056, Japan; Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima 890-0056, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26766177

Citation

Dawood, Mahmoud A O., et al. "Effects of Dietary Supplementation of Lactobacillus Rhamnosus Or/and Lactococcus Lactis On the Growth, Gut Microbiota and Immune Responses of Red Sea Bream, Pagrus Major." Fish & Shellfish Immunology, vol. 49, 2016, pp. 275-85.
Dawood MA, Koshio S, Ishikawa M, et al. Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. Fish Shellfish Immunol. 2016;49:275-85.
Dawood, M. A., Koshio, S., Ishikawa, M., Yokoyama, S., El Basuini, M. F., Hossain, M. S., Nhu, T. H., Dossou, S., & Moss, A. S. (2016). Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. Fish & Shellfish Immunology, 49, 275-85. https://doi.org/10.1016/j.fsi.2015.12.047
Dawood MA, et al. Effects of Dietary Supplementation of Lactobacillus Rhamnosus Or/and Lactococcus Lactis On the Growth, Gut Microbiota and Immune Responses of Red Sea Bream, Pagrus Major. Fish Shellfish Immunol. 2016;49:275-85. PubMed PMID: 26766177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major. AU - Dawood,Mahmoud A O, AU - Koshio,Shunsuke, AU - Ishikawa,Manabu, AU - Yokoyama,Saichiro, AU - El Basuini,Mohammed F, AU - Hossain,Md Sakhawat, AU - Nhu,Truong H, AU - Dossou,Serge, AU - Moss,Amina S, Y1 - 2016/01/04/ PY - 2015/11/10/received PY - 2015/12/30/revised PY - 2015/12/31/accepted PY - 2016/1/15/entrez PY - 2016/1/15/pubmed PY - 2016/10/22/medline KW - Feed utilization KW - Growth KW - Immunity KW - Lactobacillus rhamnosus KW - Lactococcus lactis KW - Red sea bream SP - 275 EP - 85 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 49 N2 - Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P < 0.05). Serum lysozyme activity (LZY) significantly increased in LR + LL when compared with the control group. Additionally, fish fed the LR + LL diet showed a higher growth performance (Fn wt, WG, and SGR) and protein digestibility than the groups fed an individual LR or the control diet. Superoxide dismutase (SOD) significantly increased in LR and LR + LL groups when compared with the other groups. Moreover, the fish fed LR or LL had better improvement (P < 0.05) in growth, feed utilization, body protein and lipid contents, digestibility coefficients (dry matter, protein, and lipid), protease activity, total intestine and LAB counts, hematocrit, total plasma protein, biological antioxidant potential, ACP, serum and mucus LZY and bactericidal activities, peroxidase, SOD, and mucus secretion than the control group. Interestingly, fish fed diets with LR + LL showed significantly lower total cholesterol and triglycerides when compared with the other groups (P < 0.05). These data strongly suggest that a mixture of LR and LL probiotics may serve as a healthy immunostimulating feed additive in red sea bream aquaculture. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/26766177/Effects_of_dietary_supplementation_of_Lactobacillus_rhamnosus_or/and_Lactococcus_lactis_on_the_growth_gut_microbiota_and_immune_responses_of_red_sea_bream_Pagrus_major_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(15)30305-3 DB - PRIME DP - Unbound Medicine ER -