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Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh.
PLoS Negl Trop Dis. 2016 Jan; 10(1):e0004301.PN

Abstract

BACKGROUND

Melioidosis, caused by Burkholderia pseudomallei, is an endemic disease in Bangladesh. No systematic study has yet been done to detect the environmental source of the organism and its true extent in Bangladesh. The present study attempted to isolate B. pseudomallei in soil samples and to determine its seroprevalence in several districts in Bangladesh.

METHODOLOGY AND RESULTS

Soil samples were collected from rural areas of four districts of Bangladesh from where culture confirmed melioidosis cases were detected earlier. Multiple soil samples, collected from 5-7 sampling points of 3-5 sites of each district, were cultured in Ashdown selective media. Suspected colonies of B. pseudomallei were identified by biochemical and serological test, and by polymerase chain reaction (PCR) using 16s rRNA specific primers. Blood samples were collected from 940 healthy individuals of four districts to determine anti- B. pseudomallei IgG antibody levels by indirect enzyme linked immunosorbent assay (ELISA) using sonicated crude antigen. Out of 179 soil samples, B. pseudomallei was isolated from two samples of Gazipur district which is located 58 km north of capital Dhaka city. Both the isolates were phenotypically identical, arabinose negative and showed specific 550bp band in PCR. Out of 940 blood samples, anti- B. pseudomallei IgG antibody, higher than the cut-off value (>0.8), was detected in 21.5% individuals. Seropositivity rate was 22.6%-30.8% in three districts from where melioidosis cases were detected earlier, compared to 9.8% in a district where no melioidosis case was either detected or reported (p<0.01). Seropositivity increased with the advancement of age from 5.3% to 30.4% among individuals aged 1-10 years and > 50 years respectively. The seropositivity rates were 26.0% and 20.6% in male and female respectively, while it was 20-27% among different occupational groups. No significant association was observed with gender (χ2 = 3.441, p = 0.064) or any occupational group (χ2 = 3.835, p = 0.280).

CONCLUSION

This is the first study demonstrating the presence of B. pseudomallei in the environmental (soil) samples of Bangladesh. It also suggested that a large proportion of people, residing in these districts, were exposed to the organism.

Authors+Show Affiliations

Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh.Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh.Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka, Bangladesh.Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26771511

Citation

Jilani, Md Shariful Alam, et al. "Burkholderia Pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh." PLoS Neglected Tropical Diseases, vol. 10, no. 1, 2016, pp. e0004301.
Jilani MS, Robayet JA, Mohiuddin M, et al. Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh. PLoS Negl Trop Dis. 2016;10(1):e0004301.
Jilani, M. S., Robayet, J. A., Mohiuddin, M., Hasan, M. R., Ahsan, C. R., & Haq, J. A. (2016). Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh. PLoS Neglected Tropical Diseases, 10(1), e0004301. https://doi.org/10.1371/journal.pntd.0004301
Jilani MS, et al. Burkholderia Pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh. PLoS Negl Trop Dis. 2016;10(1):e0004301. PubMed PMID: 26771511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Burkholderia pseudomallei: Its Detection in Soil and Seroprevalence in Bangladesh. AU - Jilani,Md Shariful Alam, AU - Robayet,Jamshedul Alam Mohammad, AU - Mohiuddin,Md, AU - Hasan,Md Rokib, AU - Ahsan,Chowdhury Rafiqul, AU - Haq,Jalaluddin Ashraful, Y1 - 2016/01/15/ PY - 2015/07/12/received PY - 2015/11/23/accepted PY - 2016/1/16/entrez PY - 2016/1/16/pubmed PY - 2016/6/2/medline SP - e0004301 EP - e0004301 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 10 IS - 1 N2 - BACKGROUND: Melioidosis, caused by Burkholderia pseudomallei, is an endemic disease in Bangladesh. No systematic study has yet been done to detect the environmental source of the organism and its true extent in Bangladesh. The present study attempted to isolate B. pseudomallei in soil samples and to determine its seroprevalence in several districts in Bangladesh. METHODOLOGY AND RESULTS: Soil samples were collected from rural areas of four districts of Bangladesh from where culture confirmed melioidosis cases were detected earlier. Multiple soil samples, collected from 5-7 sampling points of 3-5 sites of each district, were cultured in Ashdown selective media. Suspected colonies of B. pseudomallei were identified by biochemical and serological test, and by polymerase chain reaction (PCR) using 16s rRNA specific primers. Blood samples were collected from 940 healthy individuals of four districts to determine anti- B. pseudomallei IgG antibody levels by indirect enzyme linked immunosorbent assay (ELISA) using sonicated crude antigen. Out of 179 soil samples, B. pseudomallei was isolated from two samples of Gazipur district which is located 58 km north of capital Dhaka city. Both the isolates were phenotypically identical, arabinose negative and showed specific 550bp band in PCR. Out of 940 blood samples, anti- B. pseudomallei IgG antibody, higher than the cut-off value (>0.8), was detected in 21.5% individuals. Seropositivity rate was 22.6%-30.8% in three districts from where melioidosis cases were detected earlier, compared to 9.8% in a district where no melioidosis case was either detected or reported (p<0.01). Seropositivity increased with the advancement of age from 5.3% to 30.4% among individuals aged 1-10 years and > 50 years respectively. The seropositivity rates were 26.0% and 20.6% in male and female respectively, while it was 20-27% among different occupational groups. No significant association was observed with gender (χ2 = 3.441, p = 0.064) or any occupational group (χ2 = 3.835, p = 0.280). CONCLUSION: This is the first study demonstrating the presence of B. pseudomallei in the environmental (soil) samples of Bangladesh. It also suggested that a large proportion of people, residing in these districts, were exposed to the organism. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/26771511/Burkholderia_pseudomallei:_Its_Detection_in_Soil_and_Seroprevalence_in_Bangladesh_ L2 - http://dx.plos.org/10.1371/journal.pntd.0004301 DB - PRIME DP - Unbound Medicine ER -