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Green tea catechins prevent low-density lipoprotein oxidation via their accumulation in low-density lipoprotein particles in humans.

Abstract

Green tea is rich in polyphenols, including catechins which have antioxidant activities and are considered to have beneficial effects on cardiovascular health. In the present study, we investigated the effects of green tea catechins on low-density lipoprotein (LDL) oxidation in vitro and in human studies to test the hypothesis that catechins are incorporated into LDL particles and exert antioxidant properties. In a randomized, placebo-controlled, double-blind, crossover trial, 19 healthy men ingested green tea extract (GTE) in the form of capsules at a dose of 1 g total catechin, of which most (>99%) was the gallated type. At 1 hour after ingestion, marked increases of the plasma concentrations of (-)-epigallocatechin gallate and (-)-epicatechin gallate were observed. Accordingly, the plasma total antioxidant capacity was increased, and the LDL oxidizability was significantly reduced by the ingestion of GTE. We found that gallated catechins were incorporated into LDL particles in nonconjugated forms after the incubation of GTE with plasma in vitro. Moreover, the catechin-incorporated LDL was highly resistant to radical-induced oxidation in vitro. An additional human study with 5 healthy women confirmed that GTE intake sufficiently increased the concentration of gallated catechins, mainly in nonconjugated forms in LDL particles, and reduced the oxidizability of LDL. In conclusion, green tea catechins are rapidly incorporated into LDL particles and play a role in reducing LDL oxidation in humans, which suggests that taking green tea catechins is effective in reducing atherosclerosis risk associated with oxidative stress.

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  • Authors+Show Affiliations

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    Department of Nutrition and Food Science, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.

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    Endowed Research Department "Food for Health," Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan. Electronic address: kishimoto.yoshimi@ocha.ac.jp.

    ,

    Endowed Research Department "Food for Health," Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.

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    Endowed Research Department "Food for Health," Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.

    ,

    Central Reseach Institute, ITO EN, LTD., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan.

    ,

    Central Reseach Institute, ITO EN, LTD., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan.

    ,

    Central Reseach Institute, ITO EN, LTD., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan.

    ,

    Central Reseach Institute, ITO EN, LTD., 21 Mekami, Makinohara, Shizuoka 421-0516, Japan.

    ,

    Department of Nutrition and Food Science, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan.

    Endowed Research Department "Food for Health," Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan; Institute of Life Innovation Studies, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma 374-0193, Japan.

    Source

    MeSH

    Adult
    Antioxidants
    Atherosclerosis
    Biomarkers
    Camellia sinensis
    Catechin
    Cross-Over Studies
    Dietary Supplements
    Double-Blind Method
    Female
    Humans
    Japan
    Lipoproteins, LDL
    Male
    Middle Aged
    Oxidation-Reduction
    Plant Extracts
    Plant Leaves
    Risk Factors
    Young Adult

    Pub Type(s)

    Clinical Trial
    Journal Article
    Randomized Controlled Trial
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26773777

    Citation

    Suzuki-Sugihara, Norie, et al. "Green Tea Catechins Prevent Low-density Lipoprotein Oxidation Via Their Accumulation in Low-density Lipoprotein Particles in Humans." Nutrition Research (New York, N.Y.), vol. 36, no. 1, 2016, pp. 16-23.
    Suzuki-Sugihara N, Kishimoto Y, Saita E, et al. Green tea catechins prevent low-density lipoprotein oxidation via their accumulation in low-density lipoprotein particles in humans. Nutr Res. 2016;36(1):16-23.
    Suzuki-Sugihara, N., Kishimoto, Y., Saita, E., Taguchi, C., Kobayashi, M., Ichitani, M., ... Kondo, K. (2016). Green tea catechins prevent low-density lipoprotein oxidation via their accumulation in low-density lipoprotein particles in humans. Nutrition Research (New York, N.Y.), 36(1), pp. 16-23. doi:10.1016/j.nutres.2015.10.012.
    Suzuki-Sugihara N, et al. Green Tea Catechins Prevent Low-density Lipoprotein Oxidation Via Their Accumulation in Low-density Lipoprotein Particles in Humans. Nutr Res. 2016;36(1):16-23. PubMed PMID: 26773777.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Green tea catechins prevent low-density lipoprotein oxidation via their accumulation in low-density lipoprotein particles in humans. AU - Suzuki-Sugihara,Norie, AU - Kishimoto,Yoshimi, AU - Saita,Emi, AU - Taguchi,Chie, AU - Kobayashi,Makoto, AU - Ichitani,Masaki, AU - Ukawa,Yuuichi, AU - Sagesaka,Yuko M, AU - Suzuki,Emiko, AU - Kondo,Kazuo, Y1 - 2015/11/03/ PY - 2015/08/03/received PY - 2015/10/29/revised PY - 2015/10/30/accepted PY - 2016/1/17/entrez PY - 2016/1/17/pubmed PY - 2016/11/12/medline KW - Epigallocatechin gallate KW - Humans KW - Lipid peroxidation KW - Lipoproteins KW - Low-density lipoprotein KW - Tea SP - 16 EP - 23 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 36 IS - 1 N2 - Green tea is rich in polyphenols, including catechins which have antioxidant activities and are considered to have beneficial effects on cardiovascular health. In the present study, we investigated the effects of green tea catechins on low-density lipoprotein (LDL) oxidation in vitro and in human studies to test the hypothesis that catechins are incorporated into LDL particles and exert antioxidant properties. In a randomized, placebo-controlled, double-blind, crossover trial, 19 healthy men ingested green tea extract (GTE) in the form of capsules at a dose of 1 g total catechin, of which most (>99%) was the gallated type. At 1 hour after ingestion, marked increases of the plasma concentrations of (-)-epigallocatechin gallate and (-)-epicatechin gallate were observed. Accordingly, the plasma total antioxidant capacity was increased, and the LDL oxidizability was significantly reduced by the ingestion of GTE. We found that gallated catechins were incorporated into LDL particles in nonconjugated forms after the incubation of GTE with plasma in vitro. Moreover, the catechin-incorporated LDL was highly resistant to radical-induced oxidation in vitro. An additional human study with 5 healthy women confirmed that GTE intake sufficiently increased the concentration of gallated catechins, mainly in nonconjugated forms in LDL particles, and reduced the oxidizability of LDL. In conclusion, green tea catechins are rapidly incorporated into LDL particles and play a role in reducing LDL oxidation in humans, which suggests that taking green tea catechins is effective in reducing atherosclerosis risk associated with oxidative stress. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/26773777/Green_tea_catechins_prevent_low_density_lipoprotein_oxidation_via_their_accumulation_in_low_density_lipoprotein_particles_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(15)00269-9 DB - PRIME DP - Unbound Medicine ER -