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Electroacupuncture regulate hypothalamic-pituitary-adrenal axis and enhance hippocampal serotonin system in a rat model of depression.
Neurosci Lett. 2016 Feb 26; 615:66-71.NL

Abstract

Hypothalamic-pituitary-adrenal (HPA) axis has been implicated in the pathogenesis of depression. Dysfunction of the hippocampal serotonin (5-hydroxytryptamine, 5-HT) system has been shown to be a key factor in depression. There is growing evidence that electro-acupuncture (EA) has antidepressant-like effect. However, the effect of EA on HPA axis and hippocampal serotonin system remains unknown. In our study, we investigated the antidepressant-like effect and mechanism of EA for depression rat models. Depression in rats was induced by chronic unpredictable mild stress (CUMS). EA treatment was administered once daily to CUMS rats for 14 days. The acupoints (ST36, bilateral and CV4) were selected. Untreated CUMS rats and normal rats were used as controls. Behavioral tests including forced swim test and open-field test were performed to evaluate the antidepressant effects of EA treatment. Hypothalamic corticotropin-releasing hormone (CRH) mRNA, plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) were estimated as indices of HPA axis activity. Enzyme linked immunosorbent assay (ELISA) was performed to determine the concentrations of 5-HT in the hippocampus. Real-time PCR(RT-PCR)and Western blot were respectively used to detect the mRNA and protein levels of 5-hydroxytryptamine 1A receptor (5-HT1AR) in the hippocampus. Our results showed that EA treatment reversed the behavioral deficiency induced by CUMS in rats. EA treatment decreased CRH mRNA expression in the hypothalamic, and ACTH and CORT level in plasma, and markedly increased 5-HT concentration, 5-HT1AR (mRNA and protein) expression in the hippocampus. These results indicated that EA treatment could act on depression by modulating HPA axis and enhancing hippocampal 5-HT/5-HT1AR in CUMS Rats.

Authors+Show Affiliations

Institute of Integrative Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China.Institute of Integrative Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China.E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Institute of Integrative Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China.Institute of Integrative Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China.Institute of Integrative Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China. Electronic address: jcdong2004@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26773866

Citation

Le, Jing-jing, et al. "Electroacupuncture Regulate Hypothalamic-pituitary-adrenal Axis and Enhance Hippocampal Serotonin System in a Rat Model of Depression." Neuroscience Letters, vol. 615, 2016, pp. 66-71.
Le JJ, Yi T, Qi L, et al. Electroacupuncture regulate hypothalamic-pituitary-adrenal axis and enhance hippocampal serotonin system in a rat model of depression. Neurosci Lett. 2016;615:66-71.
Le, J. J., Yi, T., Qi, L., Li, J., Shao, L., & Dong, J. C. (2016). Electroacupuncture regulate hypothalamic-pituitary-adrenal axis and enhance hippocampal serotonin system in a rat model of depression. Neuroscience Letters, 615, 66-71. https://doi.org/10.1016/j.neulet.2016.01.004
Le JJ, et al. Electroacupuncture Regulate Hypothalamic-pituitary-adrenal Axis and Enhance Hippocampal Serotonin System in a Rat Model of Depression. Neurosci Lett. 2016 Feb 26;615:66-71. PubMed PMID: 26773866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electroacupuncture regulate hypothalamic-pituitary-adrenal axis and enhance hippocampal serotonin system in a rat model of depression. AU - Le,Jing-jing, AU - Yi,Tao, AU - Qi,Li, AU - Li,Ji, AU - Shao,Lei, AU - Dong,Jing-cheng, Y1 - 2016/01/08/ PY - 2015/11/11/received PY - 2015/12/26/revised PY - 2016/01/05/accepted PY - 2016/1/17/entrez PY - 2016/1/17/pubmed PY - 2016/7/15/medline KW - 5-Hydroxytryptamine KW - 5-Hydroxytryptamine 1A receptor KW - Depression KW - Electro-acupuncture KW - Hippocampus KW - Hypothalamic–pituitary–adrenal axis SP - 66 EP - 71 JF - Neuroscience letters JO - Neurosci Lett VL - 615 N2 - Hypothalamic-pituitary-adrenal (HPA) axis has been implicated in the pathogenesis of depression. Dysfunction of the hippocampal serotonin (5-hydroxytryptamine, 5-HT) system has been shown to be a key factor in depression. There is growing evidence that electro-acupuncture (EA) has antidepressant-like effect. However, the effect of EA on HPA axis and hippocampal serotonin system remains unknown. In our study, we investigated the antidepressant-like effect and mechanism of EA for depression rat models. Depression in rats was induced by chronic unpredictable mild stress (CUMS). EA treatment was administered once daily to CUMS rats for 14 days. The acupoints (ST36, bilateral and CV4) were selected. Untreated CUMS rats and normal rats were used as controls. Behavioral tests including forced swim test and open-field test were performed to evaluate the antidepressant effects of EA treatment. Hypothalamic corticotropin-releasing hormone (CRH) mRNA, plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT) were estimated as indices of HPA axis activity. Enzyme linked immunosorbent assay (ELISA) was performed to determine the concentrations of 5-HT in the hippocampus. Real-time PCR(RT-PCR)and Western blot were respectively used to detect the mRNA and protein levels of 5-hydroxytryptamine 1A receptor (5-HT1AR) in the hippocampus. Our results showed that EA treatment reversed the behavioral deficiency induced by CUMS in rats. EA treatment decreased CRH mRNA expression in the hypothalamic, and ACTH and CORT level in plasma, and markedly increased 5-HT concentration, 5-HT1AR (mRNA and protein) expression in the hippocampus. These results indicated that EA treatment could act on depression by modulating HPA axis and enhancing hippocampal 5-HT/5-HT1AR in CUMS Rats. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/26773866/Electroacupuncture_regulate_hypothalamic_pituitary_adrenal_axis_and_enhance_hippocampal_serotonin_system_in_a_rat_model_of_depression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(16)30004-0 DB - PRIME DP - Unbound Medicine ER -