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A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study.
J Pharm Biomed Anal. 2016 Feb 20; 120:374-82.JP

Abstract

Buddlejasaponin IV (BS-IV), a natural triterpene saponin isolated from several herbal plants, has drawn a lot of attention for its anti-inflammatory, antinociceptive, antihyperlipidemia, and antitumor activities. In this study, a simple and sensitive method for determination of BS-IV in rat plasma was developed for the first time, using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Tenacissoside I was used as an internal standard (IS). Separation was achieved on an Agilent Extend-C18 column with gradient elution using methanol-water as mobile phase at a flow rate of 400μL/min. A triple quadrupole mass spectrometer operating in the positive/negative ion-switching electrospray ionization mode with selection reaction monitoring (SRM) was used to determine BS-IV and IS transitions of 941.4→779.5 and 815.5→755.5, respectively. The lower limit of quantification was 3.00ng/mL with a linear range of 3.0-3000ng/mL. The intra- and inter-day precisions were both ≤10.4% for BS-IV, and the average intra- and inter-day accuracies ranged from -7.2% to 6.7%. The validated assay was successfully applied to a pharmacokinetic study of BS-IV following oral administration of 3, 6, 12mg/kg and an intravenous administration of 0.9mg/kg to rats.

Authors+Show Affiliations

Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.Departments of Cardiology and Echocardiography, The First Hospital of Jilin University, Changchun 130021, China.Department of Orthopedic Trauma, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: tanl_luk@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26774034

Citation

Li, Yanhui, et al. "A Simple and Sensitive UHPLC-MS/MS Method for Quantification of Buddlejasaponin IV in Rat Plasma and Its Application to a Pharmacokinetic Study." Journal of Pharmaceutical and Biomedical Analysis, vol. 120, 2016, pp. 374-82.
Li Y, Xu H, Chen L, et al. A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study. J Pharm Biomed Anal. 2016;120:374-82.
Li, Y., Xu, H., Chen, L., & Tan, L. (2016). A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis, 120, 374-82. https://doi.org/10.1016/j.jpba.2015.12.044
Li Y, et al. A Simple and Sensitive UHPLC-MS/MS Method for Quantification of Buddlejasaponin IV in Rat Plasma and Its Application to a Pharmacokinetic Study. J Pharm Biomed Anal. 2016 Feb 20;120:374-82. PubMed PMID: 26774034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple and sensitive UHPLC-MS/MS method for quantification of buddlejasaponin IV in rat plasma and its application to a pharmacokinetic study. AU - Li,Yanhui, AU - Xu,Hui, AU - Chen,Liping, AU - Tan,Lei, Y1 - 2015/12/29/ PY - 2015/09/19/received PY - 2015/12/21/revised PY - 2015/12/22/accepted PY - 2016/1/17/entrez PY - 2016/1/17/pubmed PY - 2016/12/15/medline KW - Buddlejasaponin IV KW - Pharmacokinetics KW - Rat plasma KW - UHPLC-MS/MS SP - 374 EP - 82 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 120 N2 - Buddlejasaponin IV (BS-IV), a natural triterpene saponin isolated from several herbal plants, has drawn a lot of attention for its anti-inflammatory, antinociceptive, antihyperlipidemia, and antitumor activities. In this study, a simple and sensitive method for determination of BS-IV in rat plasma was developed for the first time, using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Tenacissoside I was used as an internal standard (IS). Separation was achieved on an Agilent Extend-C18 column with gradient elution using methanol-water as mobile phase at a flow rate of 400μL/min. A triple quadrupole mass spectrometer operating in the positive/negative ion-switching electrospray ionization mode with selection reaction monitoring (SRM) was used to determine BS-IV and IS transitions of 941.4→779.5 and 815.5→755.5, respectively. The lower limit of quantification was 3.00ng/mL with a linear range of 3.0-3000ng/mL. The intra- and inter-day precisions were both ≤10.4% for BS-IV, and the average intra- and inter-day accuracies ranged from -7.2% to 6.7%. The validated assay was successfully applied to a pharmacokinetic study of BS-IV following oral administration of 3, 6, 12mg/kg and an intravenous administration of 0.9mg/kg to rats. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/26774034/A_simple_and_sensitive_UHPLC_MS/MS_method_for_quantification_of_buddlejasaponin_IV_in_rat_plasma_and_its_application_to_a_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(15)30310-1 DB - PRIME DP - Unbound Medicine ER -