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Pharmacological effects and toxicity of Costus pulverulentus C. Presl (Costaceae).
J Ethnopharmacol. 2016 Mar 02; 180:124-30.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Costus pulverulentus C. Presl (Costaceae), a species endemic to Mexico, is used for the empirical treatment of cancer, pain, and inflammation.

AIM OF THE STUDY

The objective of this study was to evaluate the toxicity, as well as the cytotoxic, antinociceptive, anti-inflammatory and sedative effects of an ethanol extract from Costus pulverulentus stem (CPE).

MATERIALS AND METHODS

The chemical characterization of CPE was performed by Gas chromatography-mass spectrometry (GC-MS). The toxicity of CPE was evaluated using the comet assay (10-1000 µg/ml during 5h) and the acute toxicity test (500-5000 mg/kg p.o. and i.p. during 14 days). The cytotoxic effect of CPE (1-250 µg/ml) on human cancer cells was evaluated using the MTT assay. The antinociceptive effects of CPE (50-200mg/kg p.o.) were evaluated using thermal-induced nociception tests (hot plate and tail flick) and the chemical-induced nociceptive tests (acetic acid and formalin). The sedative activity of CPE (50-200mg/kg p.o.) was evaluated using the ketamine-induced sleeping time test.

RESULTS

CPE showed the presence of compounds such as campesterol, stigmasterol β-sitosterol, vanillic acid, among others. In the comet assay, CPE at 200 µg/ml or higher concentrations induced DNA damage. In the acute toxicity test, the LD50 estimated for CPE was>5000 mg/kg p.o. or i.p. CEP showed moderate cytotoxic effects on prostate carcinoma cells PC-3 cells (IC50=179 ± 23.2 µg/ml). In the chemical-induced nociception models, CPE (100 and 200mg/kg p.o.) showed antinociceptive effects with similar activity to 100mg/kg naproxen. In the thermal-induced nociception tests, CPE tested at 200mg/kg showed moderate antinociceptive effects by 28% (hot plate test) and by 25% (tail flick test). In the ketamine-induced sleeping time test, CPE showed no sedative effects.

CONCLUSIONS

C. pulverulents exerts moderate cytotoxic effects in human cancer cells, moderate anti-inflammatory and antinociceptive effects. C. pulverulentus induces antinociceptive effects without inducing sedation.

Authors+Show Affiliations

Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, México. Electronic address: angeljosabad@ugto.mx.Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, México.Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.Unidad Académica Multidisciplinaria de la Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, San Luis Potosí, México. Electronic address: candy.carranza@uaslp.mx.Unidad Académica Multidisciplinaria de la Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, San Luis Potosí, México; El colegio de la Frontera Sur unidad San Cristóbal, San Cristóbal de las Casas, Chiapas, México.Unidad Académica Multidisciplinaria de la Zona Huasteca, Universidad Autónoma de San Luis Potosí, Ciudad Valles, San Luis Potosí, México.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26778604

Citation

Alonso-Castro, Angel Josabad, et al. "Pharmacological Effects and Toxicity of Costus Pulverulentus C. Presl (Costaceae)." Journal of Ethnopharmacology, vol. 180, 2016, pp. 124-30.
Alonso-Castro AJ, Zapata-Morales JR, González-Chávez MM, et al. Pharmacological effects and toxicity of Costus pulverulentus C. Presl (Costaceae). J Ethnopharmacol. 2016;180:124-30.
Alonso-Castro, A. J., Zapata-Morales, J. R., González-Chávez, M. M., Carranza-Álvarez, C., Hernández-Benavides, D. M., & Hernández-Morales, A. (2016). Pharmacological effects and toxicity of Costus pulverulentus C. Presl (Costaceae). Journal of Ethnopharmacology, 180, 124-30. https://doi.org/10.1016/j.jep.2016.01.011
Alonso-Castro AJ, et al. Pharmacological Effects and Toxicity of Costus Pulverulentus C. Presl (Costaceae). J Ethnopharmacol. 2016 Mar 2;180:124-30. PubMed PMID: 26778604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological effects and toxicity of Costus pulverulentus C. Presl (Costaceae). AU - Alonso-Castro,Angel Josabad, AU - Zapata-Morales,Juan Ramón, AU - González-Chávez,Marco Martin, AU - Carranza-Álvarez,Candy, AU - Hernández-Benavides,Diego Manuel, AU - Hernández-Morales,Alejandro, Y1 - 2016/01/14/ PY - 2015/09/05/received PY - 2016/01/13/revised PY - 2016/01/13/accepted PY - 2016/1/19/entrez PY - 2016/1/19/pubmed PY - 2016/12/15/medline KW - Antiinflammatory KW - Antinociceptive KW - Buprenorphine (PubChem CID: 644073) KW - Cancer KW - Clonazepam (PubChem CID: 2802) KW - Costus pulverulentus KW - Gas chromatography–mass spectrometry KW - Ketamine (PubChem CID: 644025) KW - Naproxen sodium (PubChem CID: 23681059) KW - Toxicity KW - and cisplatin (PubChem CID: 441203) SP - 124 EP - 30 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 180 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Costus pulverulentus C. Presl (Costaceae), a species endemic to Mexico, is used for the empirical treatment of cancer, pain, and inflammation. AIM OF THE STUDY: The objective of this study was to evaluate the toxicity, as well as the cytotoxic, antinociceptive, anti-inflammatory and sedative effects of an ethanol extract from Costus pulverulentus stem (CPE). MATERIALS AND METHODS: The chemical characterization of CPE was performed by Gas chromatography-mass spectrometry (GC-MS). The toxicity of CPE was evaluated using the comet assay (10-1000 µg/ml during 5h) and the acute toxicity test (500-5000 mg/kg p.o. and i.p. during 14 days). The cytotoxic effect of CPE (1-250 µg/ml) on human cancer cells was evaluated using the MTT assay. The antinociceptive effects of CPE (50-200mg/kg p.o.) were evaluated using thermal-induced nociception tests (hot plate and tail flick) and the chemical-induced nociceptive tests (acetic acid and formalin). The sedative activity of CPE (50-200mg/kg p.o.) was evaluated using the ketamine-induced sleeping time test. RESULTS: CPE showed the presence of compounds such as campesterol, stigmasterol β-sitosterol, vanillic acid, among others. In the comet assay, CPE at 200 µg/ml or higher concentrations induced DNA damage. In the acute toxicity test, the LD50 estimated for CPE was>5000 mg/kg p.o. or i.p. CEP showed moderate cytotoxic effects on prostate carcinoma cells PC-3 cells (IC50=179 ± 23.2 µg/ml). In the chemical-induced nociception models, CPE (100 and 200mg/kg p.o.) showed antinociceptive effects with similar activity to 100mg/kg naproxen. In the thermal-induced nociception tests, CPE tested at 200mg/kg showed moderate antinociceptive effects by 28% (hot plate test) and by 25% (tail flick test). In the ketamine-induced sleeping time test, CPE showed no sedative effects. CONCLUSIONS: C. pulverulents exerts moderate cytotoxic effects in human cancer cells, moderate anti-inflammatory and antinociceptive effects. C. pulverulentus induces antinociceptive effects without inducing sedation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26778604/Pharmacological_effects_and_toxicity_of_Costus_pulverulentus_C__Presl__Costaceae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30011-3 DB - PRIME DP - Unbound Medicine ER -