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Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts.
Environ Sci Pollut Res Int. 2016 Apr; 23(8):8008-20.ES

Abstract

Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA.

Authors+Show Affiliations

Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France. fakcha@ifremer.fr.Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.Unité Biologie des organismes et écosystèmes aquatiques (BOREA, UMR 7208), Université de Caen Normandie, Sorbonne Universités, Muséum national d'Histoire naturelle, Université Pierre et Marie Curie, CNRS, IRD, 57 rue Cuvier, 75005, Paris, France.Acobiom, 1682 rue de la Valsière, CS 77394 Cap Delta Biopole Euromédecine II, 34184, Montpellier Cedex 04, France.Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.Ifremer, UMR 5244, IHPE Interaction Host Pathogen Environment, UPVD, CNRS, Université de Montpellier, CC 80, F-34095, Montpellier, France.IDAEA-CSIC, Environmental Chemistry Department, C/ Jordi Girona, 1808034, Barcelona, Spain.IDAEA-CSIC, Environmental Chemistry Department, C/ Jordi Girona, 1808034, Barcelona, Spain.Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.Ifremer, Laboratoire de Génétique et Pathologies, Rue de Mus de Loup, La Tremblade, 17390, France.Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM), UBO/CNRS/IRD/IFREMER, rue Dumont d'Urville, technopôle, Brest-Iroise, 29280, Plouzané, France.Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.Laboratoire d'Ecotoxicologie, Ifremer, Rue de l'Ile d'Yeu, BP21105, 44311, Nantes cedex 03, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26780042

Citation

Akcha, F, et al. "Effects of an Environmentally Relevant Concentration of Diuron On Oyster Genitors During Gametogenesis: Responses of Early Molecular and Cellular Markers and Physiological Impacts." Environmental Science and Pollution Research International, vol. 23, no. 8, 2016, pp. 8008-20.
Akcha F, Barranger A, Bachère E, et al. Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. Environ Sci Pollut Res Int. 2016;23(8):8008-20.
Akcha, F., Barranger, A., Bachère, E., Berthelin, C. H., Piquemal, D., Alonso, P., Sallan, R. R., Dimastrogiovanni, G., Porte, C., Menard, D., Szczybelski, A., Benabdelmouna, A., Auffret, M., Rouxel, J., & Burgeot, T. (2016). Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. Environmental Science and Pollution Research International, 23(8), 8008-20. https://doi.org/10.1007/s11356-015-5969-2
Akcha F, et al. Effects of an Environmentally Relevant Concentration of Diuron On Oyster Genitors During Gametogenesis: Responses of Early Molecular and Cellular Markers and Physiological Impacts. Environ Sci Pollut Res Int. 2016;23(8):8008-20. PubMed PMID: 26780042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts. AU - Akcha,F, AU - Barranger,A, AU - Bachère,E, AU - Berthelin,C Heude, AU - Piquemal,D, AU - Alonso,P, AU - Sallan,R Rondon, AU - Dimastrogiovanni,G, AU - Porte,C, AU - Menard,D, AU - Szczybelski,A, AU - Benabdelmouna,A, AU - Auffret,M, AU - Rouxel,J, AU - Burgeot,T, Y1 - 2016/01/16/ PY - 2015/10/02/received PY - 2015/12/10/accepted PY - 2016/1/19/entrez PY - 2016/1/19/pubmed PY - 2017/2/23/medline KW - Gene expression KW - Histology KW - Immunity KW - Oyster KW - Pesticide KW - RT-qPCR KW - Reproduction KW - Steroid SP - 8008 EP - 20 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 23 IS - 8 N2 - Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/26780042/Effects_of_an_environmentally_relevant_concentration_of_diuron_on_oyster_genitors_during_gametogenesis:_responses_of_early_molecular_and_cellular_markers_and_physiological_impacts_ L2 - https://dx.doi.org/10.1007/s11356-015-5969-2 DB - PRIME DP - Unbound Medicine ER -