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Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC).
J Clin Virol. 2016 Feb; 75:37-41.JC

Abstract

BACKGROUND

Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (OPSCC) is a growing problem that presents a significant challenge to Otolaryngologist-Head and Neck Surgeons. Knowledge of HPV status yields critical prognostic information, with potential for treatment selection based on tumour HPV status. The current gold standard of diagnosis, PCR, is expensive, demanding and time consuming. Alternatives such as p16 immunohistochemistry are subjective and potentially inaccurate. Loop-mediated isothermal amplification (LAMP) is a rapid, robust and inexpensive molecular diagnostic technique.

OBJECTIVES

Our aim was to verify LAMP as a potential bedside diagnostic assay for subtyping of HPV in OPSCC.

STUDY DESIGN

DNA from 72 formalin-fixed paraffin embedded (FFPE) OPSCC patient samples was tested. PCR and LAMP were then performed to specifically identify HPV 16, 18, 31, 33 and 35.

RESULTS AND CONCLUSIONS

For these high-risk subtypes, LAMP had an overall sensitivity of 99.4% and specificity of 93.2% relative to PCR. LAMP is a promising technology that can accurately diagnose high-risk HPV infection.

Authors+Show Affiliations

Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: devonlivingstone@gmail.com.Undergraduate Medical Education, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, Alberta, Canada.Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.Ohlson Research Initiative, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary and Calgary Laboratory Services, Calgary, Alberta, Canada.Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada; Ohlson Research Initiative, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26780110

Citation

Livingstone, D M., et al. "Loop Mediated Isothermal Amplification (LAMP) for the Detection and Subtyping of Human Papillomaviruses (HPV) in Oropharyngeal Squamous Cell Carcinoma (OPSCC)." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 75, 2016, pp. 37-41.
Livingstone DM, Rohatensky M, Mintchev P, et al. Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC). J Clin Virol. 2016;75:37-41.
Livingstone, D. M., Rohatensky, M., Mintchev, P., Nakoneshny, S. C., Demetrick, D. J., van Marle, G., & Dort, J. C. (2016). Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC). Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 75, 37-41. https://doi.org/10.1016/j.jcv.2016.01.002
Livingstone DM, et al. Loop Mediated Isothermal Amplification (LAMP) for the Detection and Subtyping of Human Papillomaviruses (HPV) in Oropharyngeal Squamous Cell Carcinoma (OPSCC). J Clin Virol. 2016;75:37-41. PubMed PMID: 26780110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC). AU - Livingstone,D M, AU - Rohatensky,M, AU - Mintchev,P, AU - Nakoneshny,S C, AU - Demetrick,D J, AU - van Marle,G, AU - Dort,J C, Y1 - 2016/01/06/ PY - 2015/09/14/received PY - 2015/12/08/revised PY - 2016/01/05/accepted PY - 2016/1/19/entrez PY - 2016/1/19/pubmed PY - 2016/8/30/medline KW - HPV KW - LAMP KW - Loop-mediated isothermal amplification KW - OPSCC KW - Oropharynx SP - 37 EP - 41 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 75 N2 - BACKGROUND: Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (OPSCC) is a growing problem that presents a significant challenge to Otolaryngologist-Head and Neck Surgeons. Knowledge of HPV status yields critical prognostic information, with potential for treatment selection based on tumour HPV status. The current gold standard of diagnosis, PCR, is expensive, demanding and time consuming. Alternatives such as p16 immunohistochemistry are subjective and potentially inaccurate. Loop-mediated isothermal amplification (LAMP) is a rapid, robust and inexpensive molecular diagnostic technique. OBJECTIVES: Our aim was to verify LAMP as a potential bedside diagnostic assay for subtyping of HPV in OPSCC. STUDY DESIGN: DNA from 72 formalin-fixed paraffin embedded (FFPE) OPSCC patient samples was tested. PCR and LAMP were then performed to specifically identify HPV 16, 18, 31, 33 and 35. RESULTS AND CONCLUSIONS: For these high-risk subtypes, LAMP had an overall sensitivity of 99.4% and specificity of 93.2% relative to PCR. LAMP is a promising technology that can accurately diagnose high-risk HPV infection. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/26780110/Loop_mediated_isothermal_amplification__LAMP__for_the_detection_and_subtyping_of_human_papillomaviruses__HPV__in_oropharyngeal_squamous_cell_carcinoma__OPSCC__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(16)00004-4 DB - PRIME DP - Unbound Medicine ER -