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Affirm VPIII microbial identification test can be used to detect gardnerella vaginalis, Candida albicans and trichomonas vaginalis microbial infections in Korean women.
J Obstet Gynaecol Res. 2016 Apr; 42(4):422-6.JO

Abstract

AIM

The aim of this study was to compare Affirm VPIII Microbial Identification Test results for Korean women to those obtained for Gardnerella vaginalis through Nugent score, Candida albicans based on vaginal culture and Trichomonas vaginalis based on wet smear diagnostic standards.

METHODS

Study participants included 195 women with symptomatic or asymptomatic vulvovaginitis under hospital obstetric or gynecologic care. A definite diagnosis was made based on Nugent score for Gardnerella, vaginal culture for Candida and wet prep for Trichomonas vaginalis. Affirm VPIII Microbial Identification Test results were then compared to diagnostic standard results.

RESULTS

Of the 195 participants, 152 were symptomatic, while 43 were asymptomatic. Final diagnosis revealed 68 (37.87%) cases of Gardnerella, 29 (14.87%) cases of Candida, one (0.51%) case of Trichomonas, and 10 (5.10%) cases of mixed infections. The detection rates achieved by each detection method (Affirm assay vs diagnostic standard) for Gardnerella and Candida were not significantly different (33.33% vs 34.8% for Gardnerella, 13.33% vs 14.87% for Candida, respectively). The sensitivity and specificity of the Affirm test for Gardnerella compared to the diagnostic standard were 75.0% and 88.98%, respectively. For Candida, the sensitivity and specificity of the Affirm test compared to the diagnostic standard were 82.76% and 98.80%, respectively. The number of Trichomonas cases was too small (1 case) to be statistically analyzed.

CONCLUSIONS

The Affirm test is a quick tool that can help physicians diagnose and treat patients with infectious vaginitis at the point of care.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Byun SW
Department of Obstetrics and Gynecology, The Catholic University of Korea, Seoul, South Korea.
Park YJ
Department of Laboratory Medicine, The Catholic University of Korea, Seoul, South Korea.
Hur SY
Department of Obstetrics and Gynecology, The Catholic University of Korea, Seoul, South Korea.

MeSH

AdultCandida albicansCandidiasis, VulvovaginalDNA ProbesFemaleGardnerella vaginalisHumansMiddle AgedReagent Kits, DiagnosticRepublic of KoreaSensitivity and SpecificityTrichomonas VaginitisTrichomonas vaginalisVaginitisVaginosis, Bacterial

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26787446

Citation

Byun, Seung Won, et al. "Affirm VPIII Microbial Identification Test Can Be Used to Detect Gardnerella Vaginalis, Candida Albicans and Trichomonas Vaginalis Microbial Infections in Korean Women." The Journal of Obstetrics and Gynaecology Research, vol. 42, no. 4, 2016, pp. 422-6.
Byun SW, Park YJ, Hur SY. Affirm VPIII microbial identification test can be used to detect gardnerella vaginalis, Candida albicans and trichomonas vaginalis microbial infections in Korean women. J Obstet Gynaecol Res. 2016;42(4):422-6.
Byun, S. W., Park, Y. J., & Hur, S. Y. (2016). Affirm VPIII microbial identification test can be used to detect gardnerella vaginalis, Candida albicans and trichomonas vaginalis microbial infections in Korean women. The Journal of Obstetrics and Gynaecology Research, 42(4), 422-6. https://doi.org/10.1111/jog.12913
Byun SW, Park YJ, Hur SY. Affirm VPIII Microbial Identification Test Can Be Used to Detect Gardnerella Vaginalis, Candida Albicans and Trichomonas Vaginalis Microbial Infections in Korean Women. J Obstet Gynaecol Res. 2016;42(4):422-6. PubMed PMID: 26787446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Affirm VPIII microbial identification test can be used to detect gardnerella vaginalis, Candida albicans and trichomonas vaginalis microbial infections in Korean women. AU - Byun,Seung Won, AU - Park,Yeon Joon, AU - Hur,Soo Young, Y1 - 2016/01/20/ PY - 2015/06/01/received PY - 2015/09/15/revised PY - 2015/10/28/accepted PY - 2016/1/21/entrez PY - 2016/1/21/pubmed PY - 2017/1/18/medline KW - Nugent score KW - affirm test KW - culture KW - infectious vaginitis KW - wet prep + SP - 422 EP - 6 JF - The journal of obstetrics and gynaecology research JO - J Obstet Gynaecol Res VL - 42 IS - 4 N2 - AIM: The aim of this study was to compare Affirm VPIII Microbial Identification Test results for Korean women to those obtained for Gardnerella vaginalis through Nugent score, Candida albicans based on vaginal culture and Trichomonas vaginalis based on wet smear diagnostic standards. METHODS: Study participants included 195 women with symptomatic or asymptomatic vulvovaginitis under hospital obstetric or gynecologic care. A definite diagnosis was made based on Nugent score for Gardnerella, vaginal culture for Candida and wet prep for Trichomonas vaginalis. Affirm VPIII Microbial Identification Test results were then compared to diagnostic standard results. RESULTS: Of the 195 participants, 152 were symptomatic, while 43 were asymptomatic. Final diagnosis revealed 68 (37.87%) cases of Gardnerella, 29 (14.87%) cases of Candida, one (0.51%) case of Trichomonas, and 10 (5.10%) cases of mixed infections. The detection rates achieved by each detection method (Affirm assay vs diagnostic standard) for Gardnerella and Candida were not significantly different (33.33% vs 34.8% for Gardnerella, 13.33% vs 14.87% for Candida, respectively). The sensitivity and specificity of the Affirm test for Gardnerella compared to the diagnostic standard were 75.0% and 88.98%, respectively. For Candida, the sensitivity and specificity of the Affirm test compared to the diagnostic standard were 82.76% and 98.80%, respectively. The number of Trichomonas cases was too small (1 case) to be statistically analyzed. CONCLUSIONS: The Affirm test is a quick tool that can help physicians diagnose and treat patients with infectious vaginitis at the point of care. SN - 1447-0756 UR - https://www.unboundmedicine.com/medline/citation/26787446/Affirm_VPIII_microbial_identification_test_can_be_used_to_detect_gardnerella_vaginalis_Candida_albicans_and_trichomonas_vaginalis_microbial_infections_in_Korean_women_ DB - PRIME DP - Unbound Medicine ER -