Tags

Type your tag names separated by a space and hit enter

Insight into the Salivary Gland Transcriptome of Lygus lineolaris (Palisot de Beauvois).
PLoS One. 2016; 11(1):e0147197.Plos

Abstract

The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) is a polyphagous, phytophagous insect that has emerged as a major pest of cotton, alfalfa, fruits, and vegetable crops in the eastern United States and Canada. Using its piercing-sucking mouthparts, TPB employs a "lacerate and flush" feeding strategy in which saliva injected into plant tissue degrades cell wall components and lyses cells whose contents are subsequently imbibed by the TPB. It is known that a major component of TPB saliva is the polygalacturonase enzymes that degrade the pectin in the cell walls. However, not much is known about the other components of the saliva of this important pest. In this study, we explored the salivary gland transcriptome of TPB using Illumina sequencing. After in silico conversion of RNA sequences into corresponding polypeptides, 25,767 putative proteins were discovered. Of these, 19,540 (78.83%) showed significant similarity to known proteins in the either the NCBI nr or Uniprot databases. Gene ontology (GO) terms were assigned to 7,512 proteins, and 791 proteins in the sialotranscriptome of TPB were found to collectively map to 107 Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways. A total of 3,653 Pfam domains were identified in 10,421 sialotranscriptome predicted proteins resulting in 12,814 Pfam annotations; some proteins had more than one Pfam domain. Functional annotation revealed a number of salivary gland proteins that potentially facilitate degradation of host plant tissues and mitigation of the host plant defense response. These transcripts/proteins and their potential roles in TPB establishment are described.

Authors+Show Affiliations

Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America. Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America.Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America. Institute of Entomology, Biology Centre, Academy of Sciences, Branišovská 31, 370 05 České Budĕjovice, Czech Republic.Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America.Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America.Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America. Department of Plant & Soil Sciences, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America.Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, 39762, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26789269

Citation

Showmaker, Kurt C., et al. "Insight Into the Salivary Gland Transcriptome of Lygus Lineolaris (Palisot De Beauvois)." PloS One, vol. 11, no. 1, 2016, pp. e0147197.
Showmaker KC, Bednářová A, Gresham C, et al. Insight into the Salivary Gland Transcriptome of Lygus lineolaris (Palisot de Beauvois). PLoS One. 2016;11(1):e0147197.
Showmaker, K. C., Bednářová, A., Gresham, C., Hsu, C. Y., Peterson, D. G., & Krishnan, N. (2016). Insight into the Salivary Gland Transcriptome of Lygus lineolaris (Palisot de Beauvois). PloS One, 11(1), e0147197. https://doi.org/10.1371/journal.pone.0147197
Showmaker KC, et al. Insight Into the Salivary Gland Transcriptome of Lygus Lineolaris (Palisot De Beauvois). PLoS One. 2016;11(1):e0147197. PubMed PMID: 26789269.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insight into the Salivary Gland Transcriptome of Lygus lineolaris (Palisot de Beauvois). AU - Showmaker,Kurt C, AU - Bednářová,Andrea, AU - Gresham,Cathy, AU - Hsu,Chuan-Yu, AU - Peterson,Daniel G, AU - Krishnan,Natraj, Y1 - 2016/01/20/ PY - 2015/08/30/received PY - 2015/12/30/accepted PY - 2016/1/21/entrez PY - 2016/1/21/pubmed PY - 2016/8/12/medline SP - e0147197 EP - e0147197 JF - PloS one JO - PLoS One VL - 11 IS - 1 N2 - The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) is a polyphagous, phytophagous insect that has emerged as a major pest of cotton, alfalfa, fruits, and vegetable crops in the eastern United States and Canada. Using its piercing-sucking mouthparts, TPB employs a "lacerate and flush" feeding strategy in which saliva injected into plant tissue degrades cell wall components and lyses cells whose contents are subsequently imbibed by the TPB. It is known that a major component of TPB saliva is the polygalacturonase enzymes that degrade the pectin in the cell walls. However, not much is known about the other components of the saliva of this important pest. In this study, we explored the salivary gland transcriptome of TPB using Illumina sequencing. After in silico conversion of RNA sequences into corresponding polypeptides, 25,767 putative proteins were discovered. Of these, 19,540 (78.83%) showed significant similarity to known proteins in the either the NCBI nr or Uniprot databases. Gene ontology (GO) terms were assigned to 7,512 proteins, and 791 proteins in the sialotranscriptome of TPB were found to collectively map to 107 Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways. A total of 3,653 Pfam domains were identified in 10,421 sialotranscriptome predicted proteins resulting in 12,814 Pfam annotations; some proteins had more than one Pfam domain. Functional annotation revealed a number of salivary gland proteins that potentially facilitate degradation of host plant tissues and mitigation of the host plant defense response. These transcripts/proteins and their potential roles in TPB establishment are described. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26789269/Insight_into_the_Salivary_Gland_Transcriptome_of_Lygus_lineolaris__Palisot_de_Beauvois__ L2 - https://dx.plos.org/10.1371/journal.pone.0147197 DB - PRIME DP - Unbound Medicine ER -