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The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.
J Ind Microbiol Biotechnol 2016; 43(4):441-50JI

Abstract

The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance.

Authors+Show Affiliations

United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA. Siqing.liu@ars.usda.gov.United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.United States Department of Agriculture, Bioenergy Research unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.United States Department of Agriculture, Renewable Product Technology unit, National Center for Agricultural Utilization Research, Agricultural Research Service, 1815 N. University St., Peoria, IL, 61604, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26790414

Citation

Liu, Siqing, et al. "The yajC Gene From Lactobacillus Buchneri and Escherichia Coli and Its Role in Ethanol Tolerance." Journal of Industrial Microbiology & Biotechnology, vol. 43, no. 4, 2016, pp. 441-50.
Liu S, Skory C, Qureshi N, et al. The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance. J Ind Microbiol Biotechnol. 2016;43(4):441-50.
Liu, S., Skory, C., Qureshi, N., & Hughes, S. (2016). The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance. Journal of Industrial Microbiology & Biotechnology, 43(4), pp. 441-50. doi:10.1007/s10295-015-1730-6.
Liu S, et al. The yajC Gene From Lactobacillus Buchneri and Escherichia Coli and Its Role in Ethanol Tolerance. J Ind Microbiol Biotechnol. 2016;43(4):441-50. PubMed PMID: 26790414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance. AU - Liu,Siqing, AU - Skory,Chris, AU - Qureshi,Nasib, AU - Hughes,Stephen, Y1 - 2016/01/20/ PY - 2015/09/03/received PY - 2015/12/24/accepted PY - 2016/1/22/entrez PY - 2016/1/23/pubmed PY - 2016/12/15/medline KW - Escherichia coli KW - Ethanol tolerance KW - Lactobacillus buchneri KW - Membrane protein KW - yajC SP - 441 EP - 50 JF - Journal of industrial microbiology & biotechnology JO - J. Ind. Microbiol. Biotechnol. VL - 43 IS - 4 N2 - The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl β-D-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance. SN - 1476-5535 UR - https://www.unboundmedicine.com/medline/citation/26790414/The_yajC_gene_from_Lactobacillus_buchneri_and_Escherichia_coli_and_its_role_in_ethanol_tolerance_ L2 - http://dx.doi.org/10.1007/s10295-015-1730-6 DB - PRIME DP - Unbound Medicine ER -