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A Transgenic Camelina sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice.
J Nutr. 2016 Feb; 146(2):227-35.JN

Abstract

BACKGROUND

Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed.

OBJECTIVE

The main objective was to investigate the ability of genetically engineered Camelina sativa (20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals.

METHODS

Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil-containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined.

RESULTS

The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P < 0.001). Significantly lower (36-38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P < 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P < 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P < 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P < 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator-activated receptor α (Ppara), and peroxisome proliferator-activated receptor γ (Pparg) (P < 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident.

CONCLUSIONS

Oil from transgenic Camelina is a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial.

Authors+Show Affiliations

Department of Nutrition, Norwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom; n.tejera-hernandez@uea.ac.uk.Department of Nutrition, Norwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom; Institute of Food Research, Norwich Research Park, Norwich, United Kingdom;Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling, United Kingdom;Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom; and.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom; and.Department of Nutrition, Norwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom;Institute of Food Research, Norwich Research Park, Norwich, United Kingdom;Department of Agricultural Production, School of Agricultural Engineering, Technical University of Madrid, Madrid, Spain.Department of Agricultural Production, School of Agricultural Engineering, Technical University of Madrid, Madrid, Spain.Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling, United Kingdom;Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom; and.Department of Nutrition, Norwich Medical School, Faculty of Medicine and Health Sciences, University of East Anglia, Norwich, United Kingdom;

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26791554

Citation

Tejera, Noemi, et al. "A Transgenic Camelina Sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice." The Journal of Nutrition, vol. 146, no. 2, 2016, pp. 227-35.
Tejera N, Vauzour D, Betancor MB, et al. A Transgenic Camelina sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice. J Nutr. 2016;146(2):227-35.
Tejera, N., Vauzour, D., Betancor, M. B., Sayanova, O., Usher, S., Cochard, M., Rigby, N., Ruiz-Lopez, N., Menoyo, D., Tocher, D. R., Napier, J. A., & Minihane, A. M. (2016). A Transgenic Camelina sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice. The Journal of Nutrition, 146(2), 227-35. https://doi.org/10.3945/jn.115.223941
Tejera N, et al. A Transgenic Camelina Sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice. J Nutr. 2016;146(2):227-35. PubMed PMID: 26791554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Transgenic Camelina sativa Seed Oil Effectively Replaces Fish Oil as a Dietary Source of Eicosapentaenoic Acid in Mice. AU - Tejera,Noemi, AU - Vauzour,David, AU - Betancor,Monica B, AU - Sayanova,Olga, AU - Usher,Sarah, AU - Cochard,Marianne, AU - Rigby,Neil, AU - Ruiz-Lopez,Noemi, AU - Menoyo,David, AU - Tocher,Douglas R, AU - Napier,Johnathan A, AU - Minihane,Anne Marie, Y1 - 2016/01/20/ PY - 2015/09/11/received PY - 2015/12/08/accepted PY - 2016/1/22/entrez PY - 2016/1/23/pubmed PY - 2016/6/10/medline KW - Camelina oil KW - DHA KW - EPA KW - Fads KW - TG sn-2 KW - desaturation KW - fish oil KW - n–3 PUFA KW - sustainability KW - transgenic SP - 227 EP - 35 JF - The Journal of nutrition JO - J. Nutr. VL - 146 IS - 2 N2 - BACKGROUND: Fish currently supplies only 40% of the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) required to allow all individuals globally to meet the minimum intake recommendation of 500 mg/d. Therefore, alternative sustainable sources are needed. OBJECTIVE: The main objective was to investigate the ability of genetically engineered Camelina sativa (20% EPA) oil (CO) to enrich tissue EPA and DHA relative to an EPA-rich fish oil (FO) in mammals. METHODS: Six-week-old male C57BL/6J mice were fed for 10 wk either a palm oil-containing control (C) diet or diets supplemented with EPA-CO or FO, with the C, low-EPA CO (COL), high-EPA CO (COH), low-EPA FO (FOL), and high-EPA FO (FOH) diets providing 0, 0.4, 3.4, 0.3, and 2.9 g EPA/kg diet, respectively. Liver, muscle, and brain were collected for fatty acid analysis, and blood glucose and serum lipids were quantified. The expression of selected hepatic genes involved in EPA and DHA biosynthesis and in modulating their cellular impact was determined. RESULTS: The oils were well tolerated, with significantly greater weight gain in the COH and FOH groups relative to the C group (P < 0.001). Significantly lower (36-38%) blood glucose concentrations were evident in the FOH and COH mice relative to C mice (P < 0.01). Hepatic EPA concentrations were higher in all EPA groups relative to the C group (P < 0.001), with concentrations of 0.0, 0.4, 2.9, 0.2, and 3.6 g/100 g liver total lipids in the C, COL, COH, FOL, and FOH groups, respectively. Comparable dose-independent enrichments of liver DHA were observed in mice fed CO and FO diets (P < 0.001). Relative to the C group, lower fatty acid desaturase 1 (Fads1) expression (P < 0.005) was observed in the COH and FOH groups. Higher fatty acid desaturase 2 (Fads2), peroxisome proliferator-activated receptor α (Ppara), and peroxisome proliferator-activated receptor γ (Pparg) (P < 0.005) expressions were induced by CO. No impact of treatment on liver X receptor α (Lxra) or sterol regulatory element-binding protein 1c (Srebp1c) was evident. CONCLUSIONS: Oil from transgenic Camelina is a bioavailable source of EPA in mice. These data provide support for the future assessment of this oil in a human feeding trial. SN - 1541-6100 UR - https://www.unboundmedicine.com/medline/citation/26791554/A_Transgenic_Camelina_sativa_Seed_Oil_Effectively_Replaces_Fish_Oil_as_a_Dietary_Source_of_Eicosapentaenoic_Acid_in_Mice_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.3945/jn.115.223941 DB - PRIME DP - Unbound Medicine ER -