Tags

Type your tag names separated by a space and hit enter

Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells.
Appl Environ Microbiol. 1989 Sep; 55(9):2414-5.AE

Abstract

At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose (H.M. Giaeyer, B.O. Styrvold, I. Kaasen, and A.R. Strøm, J. Bacteriol. 170:2841-2849, 1988). This reaction was exploited to preparatively synthesize [14C]trehalose from exogenous [14C]glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%.

Authors+Show Affiliations

Department of Biology, University of Konstanz, Federal Republic of Germany.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2679387

Citation

Brand, B, and W Boos. "Convenient Preparative Synthesis of [14C]trehalose From [14C]glucose By Intact Escherichia Coli Cells." Applied and Environmental Microbiology, vol. 55, no. 9, 1989, pp. 2414-5.
Brand B, Boos W. Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells. Appl Environ Microbiol. 1989;55(9):2414-5.
Brand, B., & Boos, W. (1989). Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells. Applied and Environmental Microbiology, 55(9), 2414-5.
Brand B, Boos W. Convenient Preparative Synthesis of [14C]trehalose From [14C]glucose By Intact Escherichia Coli Cells. Appl Environ Microbiol. 1989;55(9):2414-5. PubMed PMID: 2679387.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Convenient preparative synthesis of [14C]trehalose from [14C]glucose by intact Escherichia coli cells. AU - Brand,B, AU - Boos,W, PY - 1989/9/1/pubmed PY - 1989/9/1/medline PY - 1989/9/1/entrez SP - 2414 EP - 5 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 55 IS - 9 N2 - At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose (H.M. Giaeyer, B.O. Styrvold, I. Kaasen, and A.R. Strøm, J. Bacteriol. 170:2841-2849, 1988). This reaction was exploited to preparatively synthesize [14C]trehalose from exogenous [14C]glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/2679387/Convenient_preparative_synthesis_of_[14C]trehalose_from_[14C]glucose_by_intact_Escherichia_coli_cells_ L2 - https://journals.asm.org/doi/10.1128/aem.55.9.2414-2415.1989?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -