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Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes.
Int J Mol Sci. 2016 Jan 18; 17(1)IJ

Abstract

The transference and reactivity of proanthocyanidins is an important issue that affects the technological processing of some fruits, such as grapes and apples. These processes are affected by proanthocyanidins bound to cell wall polysaccharides, which are present in high concentrations during the processing of the fruits. Therefore, the effective extraction of proanthocyanidins from fruits to their juices or derived products will depend on the ability to manage these associations, and, in this respect, enzymes that degrade these polysaccharides could play an important role. The main objective of this work was to test the role of pure hydrolytic enzymes (polygalacturonase and cellulose) and a commercial enzyme containing these two activities on the extent of proanthocyanidin-cell wall interactions. The results showed that the modification promoted by enzymes reduced the amount of proanthocyanidins adsorbed to cell walls since they contributed to the degradation and release of the cell wall polysaccharides, which diffused into the model solution. Some of these released polysaccharides also presented some reactivity towards the proanthocyanidins present in a model solution.

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Authors+Show Affiliations

Bautista-Ortín AB
Department of Food Science and Technology, Faculty of Veterinary, University of Murcia, 30071 Murcia, Spain. anabel@um.es.
Ben Abdallah R
Université de Tunis El Manar, Faculté des Sciences de Tunis, 2092 Tunis, Tunisia. rima_bio@hotmail.com. LIP-MB, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, 2046 Tunis, Tunisia. rima_bio@hotmail.com.
Castro-López Ldel R
Departamento de Ingeniería Química, Universidad de las Américas Puebla, Alimentos y Ambiental, Sta. Catarina Mártir, 72810 San Andrés Cholula, Puebla, México. lilianar.castrolz@udlap.mx.
Jiménez-Martínez MD
Department of Food Science and Technology, Faculty of Veterinary, University of Murcia, 30071 Murcia, Spain. mariadolores.jimenezm@um.es.
Gómez-Plaza E
Department of Food Science and Technology, Faculty of Veterinary, University of Murcia, 30071 Murcia, Spain. encarnag@um.es.

MeSH

Cell WallCellulaseChromatography, GelFood TechnologyFruitHydrolysisMalusPlant ExtractsPolygalacturonasePolysaccharidesProanthocyanidinsVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26797601

Citation

Bautista-Ortín, Ana Belén, et al. "Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes." International Journal of Molecular Sciences, vol. 17, no. 1, 2016.
Bautista-Ortín AB, Ben Abdallah R, Castro-López Ldel R, et al. Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes. Int J Mol Sci. 2016;17(1).
Bautista-Ortín, A. B., Ben Abdallah, R., Castro-López, L. d. e. l. . R., Jiménez-Martínez, M. D., & Gómez-Plaza, E. (2016). Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes. International Journal of Molecular Sciences, 17(1). https://doi.org/10.3390/ijms17010123
Bautista-Ortín AB, et al. Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes. Int J Mol Sci. 2016 Jan 18;17(1) PubMed PMID: 26797601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Technological Implications of Modifying the Extent of Cell Wall-Proanthocyanidin Interactions Using Enzymes. AU - Bautista-Ortín,Ana Belén, AU - Ben Abdallah,Rim, AU - Castro-López,Liliana Del Rocío, AU - Jiménez-Martínez,María Dolores, AU - Gómez-Plaza,Encarna, Y1 - 2016/01/18/ PY - 2015/12/16/received PY - 2016/01/08/revised PY - 2016/01/11/accepted PY - 2016/1/23/entrez PY - 2016/1/23/pubmed PY - 2016/10/19/medline KW - cell wall KW - cellulase KW - enzymes KW - phloroglucionolysis KW - polygalacturonase KW - polysaccharides KW - proanthocyanidin KW - size exclusion chromatography KW - tannins JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 1 N2 - The transference and reactivity of proanthocyanidins is an important issue that affects the technological processing of some fruits, such as grapes and apples. These processes are affected by proanthocyanidins bound to cell wall polysaccharides, which are present in high concentrations during the processing of the fruits. Therefore, the effective extraction of proanthocyanidins from fruits to their juices or derived products will depend on the ability to manage these associations, and, in this respect, enzymes that degrade these polysaccharides could play an important role. The main objective of this work was to test the role of pure hydrolytic enzymes (polygalacturonase and cellulose) and a commercial enzyme containing these two activities on the extent of proanthocyanidin-cell wall interactions. The results showed that the modification promoted by enzymes reduced the amount of proanthocyanidins adsorbed to cell walls since they contributed to the degradation and release of the cell wall polysaccharides, which diffused into the model solution. Some of these released polysaccharides also presented some reactivity towards the proanthocyanidins present in a model solution. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/26797601/Technological_Implications_of_Modifying_the_Extent_of_Cell_Wall_Proanthocyanidin_Interactions_Using_Enzymes_ DB - PRIME DP - Unbound Medicine ER -