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In situ stable isotope probing of phosphate-solubilizing bacteria in the hyphosphere.
J Exp Bot. 2016 Mar; 67(6):1689-701.JE

Abstract

This study used a [(13)C]DNA stable isotope probing (SIP) technique to elucidate a direct pathway for the translocation of (13)C-labeled photoassimilate from maize plants to extraradical mycelium-associated phosphate-solubilizing bacteria (PSB) that mediate the mineralization and turnover of soil organic phosphorus (P) in the hyphosphere. Inoculation with PSB alone did not provide any benefit to maize plants but utilized the added phytate-P to their own advantage, while inoculation with Rhizophagus irregularis alone significantly promoted shoot biomass and P content compared with the control. However, compared with both sole inoculation treatments, combined inoculation with PSB and R. irregularis in the hyphosphere enhanced organic P mineralization and increased microbial biomass P in the soil. There was no extra benefit to plant P uptake but the hyphal growth of R. irregularis was reduced, suggesting that PSB benefited from the arbuscular mycorrhizal (AM) fungal mycelium and competed for soil P with the fungus. The combination of T-RFLP (terminal restriction fragment length polymorphism) analysis with a clone library revealed that one of the bacteria that actively assimilated carbon derived from pulse-labeled maize plants was Pseudomonas alcaligenes (Pseudomonadaceae) that was initially inoculated into the hyphosphere soil. These results provide the first in situ demonstration of the pathway underlying the carbon flux from plants to the AM mycelium-associated PSB, and the PSB assimilated the photosynthates exuded by the fungus and promoted mineralization and turnover of organic P in the soil.

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Authors+Show Affiliations

Wang F
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China.
Shi N
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China.
Jiang R
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China.
Zhang F
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China.
Feng G
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, PR China fenggu@cau.edu.cn.

MeSH

BacteriaBiomassCarbohydrate MetabolismCarbon DioxideCarbon IsotopesHydrogen-Ion ConcentrationHyphaeIsotope LabelingMycorrhizaePhosphatesPhosphorusPhylogenyPlant ShootsPolymorphism, Restriction Fragment LengthRNA, RibosomalSodium BicarbonateSoilSolubilityZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26802172

Citation

Wang, Fei, et al. "In Situ Stable Isotope Probing of Phosphate-solubilizing Bacteria in the Hyphosphere." Journal of Experimental Botany, vol. 67, no. 6, 2016, pp. 1689-701.
Wang F, Shi N, Jiang R, et al. In situ stable isotope probing of phosphate-solubilizing bacteria in the hyphosphere. J Exp Bot. 2016;67(6):1689-701.
Wang, F., Shi, N., Jiang, R., Zhang, F., & Feng, G. (2016). In situ stable isotope probing of phosphate-solubilizing bacteria in the hyphosphere. Journal of Experimental Botany, 67(6), 1689-701. https://doi.org/10.1093/jxb/erv561
Wang F, et al. In Situ Stable Isotope Probing of Phosphate-solubilizing Bacteria in the Hyphosphere. J Exp Bot. 2016;67(6):1689-701. PubMed PMID: 26802172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ stable isotope probing of phosphate-solubilizing bacteria in the hyphosphere. AU - Wang,Fei, AU - Shi,Ning, AU - Jiang,Rongfeng, AU - Zhang,Fusuo, AU - Feng,Gu, Y1 - 2016/01/21/ PY - 2016/1/24/entrez PY - 2016/1/24/pubmed PY - 2016/12/15/medline KW - 13CO2 pulse labeling KW - AM fungus KW - hyphosphere KW - maize KW - organic phosphate KW - phosphate-solubilizing bacteria (PSB). SP - 1689 EP - 701 JF - Journal of experimental botany JO - J Exp Bot VL - 67 IS - 6 N2 - This study used a [(13)C]DNA stable isotope probing (SIP) technique to elucidate a direct pathway for the translocation of (13)C-labeled photoassimilate from maize plants to extraradical mycelium-associated phosphate-solubilizing bacteria (PSB) that mediate the mineralization and turnover of soil organic phosphorus (P) in the hyphosphere. Inoculation with PSB alone did not provide any benefit to maize plants but utilized the added phytate-P to their own advantage, while inoculation with Rhizophagus irregularis alone significantly promoted shoot biomass and P content compared with the control. However, compared with both sole inoculation treatments, combined inoculation with PSB and R. irregularis in the hyphosphere enhanced organic P mineralization and increased microbial biomass P in the soil. There was no extra benefit to plant P uptake but the hyphal growth of R. irregularis was reduced, suggesting that PSB benefited from the arbuscular mycorrhizal (AM) fungal mycelium and competed for soil P with the fungus. The combination of T-RFLP (terminal restriction fragment length polymorphism) analysis with a clone library revealed that one of the bacteria that actively assimilated carbon derived from pulse-labeled maize plants was Pseudomonas alcaligenes (Pseudomonadaceae) that was initially inoculated into the hyphosphere soil. These results provide the first in situ demonstration of the pathway underlying the carbon flux from plants to the AM mycelium-associated PSB, and the PSB assimilated the photosynthates exuded by the fungus and promoted mineralization and turnover of organic P in the soil. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/26802172/In_situ_stable_isotope_probing_of_phosphate_solubilizing_bacteria_in_the_hyphosphere_ DB - PRIME DP - Unbound Medicine ER -