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Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae.
Molecules. 2016 Jan 21; 21(1):E119.M

Abstract

Antiproliferative and antioxidant activities and mycosporine-like amino acid (MAA) profiles of methanol extracts from edible wild-harvested (Chondrus crispus, Mastocarpus stellatus, Palmaria palmata) and cultivated (C. crispus) marine red macroalgae were studied herein. Palythine, asterina-330, shinorine, palythinol, porphyra-334 and usujirene MAAs were identified in the macroalgal extracts by LC/MS/MS. Extract reducing activity rankings were (p < 0.001): wild P. palmata > cultivated C. crispus = wild M. stellatus > wild low-UV C. crispus > wild high-UV C. crispus; whereas oxygen radical absorbance capacities were (p < 0.001): wild M. stellatus > wild P. palmata > cultivated C. crispus > wild low-UV C. crispus > wild high-UV C. crispus. Extracts were antiproliferative against HeLa and U-937 cells (p < 0.001) from 0.125-4 mg/mL, 24 h. Wild P. palmata and cultivated C. crispus extracts increased (p < 0.001) HeLa caspase-3/7 activities and the proportion of cells arrested at Sub G₁ (apoptotic) compared to wild-harvested C. crispus and M. stellatus extracts. HeLa cells incubated with wild P. palmata and cultivated C. crispus extracts also exhibited morphological changes characteristic of apoptosis (shrinkage, rounding). Thus, extracts rich in low-polarity usujirene and polar palythine and asterina-330 MAAs were antiproliferative as inducers of apoptosis in HeLa cells.

Authors+Show Affiliations

School of Nutrition, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada. yasantha7@yahoo.com.School of Nutrition, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada. susan.trang1@gmail.com.School of Nutrition, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada. wckwok@ryerson.ca.School of Nutrition, Ryerson University, 350 Victoria St., Toronto, ON M5B 2K3, Canada. yyuan@ryerson.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26805798

Citation

Athukorala, Yasantha, et al. "Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae." Molecules (Basel, Switzerland), vol. 21, no. 1, 2016, pp. E119.
Athukorala Y, Trang S, Kwok C, et al. Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae. Molecules. 2016;21(1):E119.
Athukorala, Y., Trang, S., Kwok, C., & Yuan, Y. V. (2016). Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae. Molecules (Basel, Switzerland), 21(1), E119. https://doi.org/10.3390/molecules21010119
Athukorala Y, et al. Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae. Molecules. 2016 Jan 21;21(1):E119. PubMed PMID: 26805798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiproliferative and Antioxidant Activities and Mycosporine-Like Amino Acid Profiles of Wild-Harvested and Cultivated Edible Canadian Marine Red Macroalgae. AU - Athukorala,Yasantha, AU - Trang,Susan, AU - Kwok,Carmen, AU - Yuan,Yvonne V, Y1 - 2016/01/21/ PY - 2015/12/22/received PY - 2016/01/13/revised PY - 2016/01/14/accepted PY - 2016/1/26/entrez PY - 2016/1/26/pubmed PY - 2016/10/21/medline KW - Chondrus crispus KW - HeLa cells KW - Mastocarpus stellatus KW - Palmaria palmata KW - U-937 cells KW - antioxidant activity KW - antiproliferative activity KW - apoptosis KW - caspase 3/7 KW - mycosporine-like amino acids (MAAs) SP - E119 EP - E119 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 21 IS - 1 N2 - Antiproliferative and antioxidant activities and mycosporine-like amino acid (MAA) profiles of methanol extracts from edible wild-harvested (Chondrus crispus, Mastocarpus stellatus, Palmaria palmata) and cultivated (C. crispus) marine red macroalgae were studied herein. Palythine, asterina-330, shinorine, palythinol, porphyra-334 and usujirene MAAs were identified in the macroalgal extracts by LC/MS/MS. Extract reducing activity rankings were (p < 0.001): wild P. palmata > cultivated C. crispus = wild M. stellatus > wild low-UV C. crispus > wild high-UV C. crispus; whereas oxygen radical absorbance capacities were (p < 0.001): wild M. stellatus > wild P. palmata > cultivated C. crispus > wild low-UV C. crispus > wild high-UV C. crispus. Extracts were antiproliferative against HeLa and U-937 cells (p < 0.001) from 0.125-4 mg/mL, 24 h. Wild P. palmata and cultivated C. crispus extracts increased (p < 0.001) HeLa caspase-3/7 activities and the proportion of cells arrested at Sub G₁ (apoptotic) compared to wild-harvested C. crispus and M. stellatus extracts. HeLa cells incubated with wild P. palmata and cultivated C. crispus extracts also exhibited morphological changes characteristic of apoptosis (shrinkage, rounding). Thus, extracts rich in low-polarity usujirene and polar palythine and asterina-330 MAAs were antiproliferative as inducers of apoptosis in HeLa cells. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/26805798/Antiproliferative_and_Antioxidant_Activities_and_Mycosporine_Like_Amino_Acid_Profiles_of_Wild_Harvested_and_Cultivated_Edible_Canadian_Marine_Red_Macroalgae_ DB - PRIME DP - Unbound Medicine ER -