Tags

Type your tag names separated by a space and hit enter

Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract.
J Sep Sci. 2016 Jan; 39(2):296-305.JS

Abstract

Pharmacokinetic studies were conducted on rats for protopine, corynoline, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)-ethyl]propenamide, acetylcorynoline, and 8-oxocorynoline, five main active components from Corydalis bungeana Turcz (C. bungeana Turcz). An ultra high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous determination of the pharmacokinetic characteristics of these components in rat plasma. Chromatographic separation was achieved on an Agilent SB-C18 column (1.8 μm, 150 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% acetic acid at a flow rate of 0.3 mL/min. The analytes were detected in the multiple reaction monitoring mode with positive electrospray ionization. Lower limits of quantification were >0.680 ng/mL and matrix effects ranged from 91.26 to 100.38%. The mean extraction recoveries of quality control samples were less than 79.32%, and the precision and accuracy were within the acceptable limits. All analytes were proven to be stable during sample storage and analysis procedures. The validated method was successfully applied to the pharmacokinetic study of five alkaloid components after oral administration of C. bungeana Turcz extract to rats. The obtained results may be helpful to reveal the mechanism of action and to guide the clinical application of C. bungeana Turcz.

Authors+Show Affiliations

College of Pharmacy, Harbin Medical University, Harbin, China.College of Pharmacy, Harbin Medical University, Harbin, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, China.College of Pharmacy, Harbin Medical University, Harbin, China.College of Pharmacy, Harbin Medical University, Harbin, China.College of Pharmacy, Harbin Medical University, Harbin, China.College of Pharmacy, Harbin Medical University, Harbin, China.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26805956

Citation

Liu, Lu, et al. "Simultaneous Determination and Pharmacokinetics of Five Alkaloids in Rat Plasma By Ultra High Performance Liquid Chromatography With Tandem Mass Spectrometry After the Oral Administration of Corydalis Bungeana Turcz Extract." Journal of Separation Science, vol. 39, no. 2, 2016, pp. 296-305.
Liu L, Yu X, Wang Z, et al. Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract. J Sep Sci. 2016;39(2):296-305.
Liu, L., Yu, X., Wang, Z., Sun, J., Pan, R., Yang, C., & Wu, L. (2016). Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract. Journal of Separation Science, 39(2), 296-305. https://doi.org/10.1002/jssc.201500889
Liu L, et al. Simultaneous Determination and Pharmacokinetics of Five Alkaloids in Rat Plasma By Ultra High Performance Liquid Chromatography With Tandem Mass Spectrometry After the Oral Administration of Corydalis Bungeana Turcz Extract. J Sep Sci. 2016;39(2):296-305. PubMed PMID: 26805956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination and pharmacokinetics of five alkaloids in rat plasma by ultra high performance liquid chromatography with tandem mass spectrometry after the oral administration of Corydalis bungeana Turcz extract. AU - Liu,Lu, AU - Yu,Xueli, AU - Wang,Zhibin, AU - Sun,Jiahui, AU - Pan,Rong, AU - Yang,Chunjuan, AU - Wu,Lijun, Y1 - 2015/11/26/ PY - 2015/08/10/received PY - 2015/10/05/revised PY - 2015/10/21/accepted PY - 2016/1/26/entrez PY - 2016/1/26/pubmed PY - 2016/10/19/medline KW - Alkaloids KW - Corydalis bungeana Turcz KW - Pharmacokinetics KW - Tandem mass spectrometry KW - Ultra high performance liquid chromatography SP - 296 EP - 305 JF - Journal of separation science JO - J Sep Sci VL - 39 IS - 2 N2 - Pharmacokinetic studies were conducted on rats for protopine, corynoline, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)-ethyl]propenamide, acetylcorynoline, and 8-oxocorynoline, five main active components from Corydalis bungeana Turcz (C. bungeana Turcz). An ultra high performance liquid chromatography with tandem mass spectrometry method has been developed and validated for the simultaneous determination of the pharmacokinetic characteristics of these components in rat plasma. Chromatographic separation was achieved on an Agilent SB-C18 column (1.8 μm, 150 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% acetic acid at a flow rate of 0.3 mL/min. The analytes were detected in the multiple reaction monitoring mode with positive electrospray ionization. Lower limits of quantification were >0.680 ng/mL and matrix effects ranged from 91.26 to 100.38%. The mean extraction recoveries of quality control samples were less than 79.32%, and the precision and accuracy were within the acceptable limits. All analytes were proven to be stable during sample storage and analysis procedures. The validated method was successfully applied to the pharmacokinetic study of five alkaloid components after oral administration of C. bungeana Turcz extract to rats. The obtained results may be helpful to reveal the mechanism of action and to guide the clinical application of C. bungeana Turcz. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/26805956/Simultaneous_determination_and_pharmacokinetics_of_five_alkaloids_in_rat_plasma_by_ultra_high_performance_liquid_chromatography_with_tandem_mass_spectrometry_after_the_oral_administration_of_Corydalis_bungeana_Turcz_extract_ L2 - https://doi.org/10.1002/jssc.201500889 DB - PRIME DP - Unbound Medicine ER -