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Cilostazol attenuates gentamicin-induced nephrotoxicity in rats.
Exp Toxicol Pathol. 2016 Apr; 68(4):247-53.ET

Abstract

INTRODUCTION

Gentamycin is a widely used antibiotic. The nephrotoxic adverse effects of the drug may limit its use. Cilostazol, a phosphodiesterase III inhibitor, was reported to protect from renal oxidative stress. This work aimed to investigate the possible protective effect of cilostazol on gentamicin-induced nephrotoxicity and the possible underlying mechanisms.

MATERIALS AND METHODS

40 male albino rats were divided into 4 equal groups: (1) Control; (2) Cilostazol, 10mg/kg, p.o.; (3) Gentamicin, 80 mg/kg, i.p.; (4) Gentamicin 80 mg/kg, i.p. along with cilostazol 10mg/kg, p.o. All drugs were administered once daily for 8 days. On 9th day blood samples were collected for the estimation of creatinine, urea and uric acid in serum. Then the rats were sacrificed and kidneys were removed for light and electron microscope studies. Moreover, reduced glutathione (GSH) and malondialdehyde (MDA) levels as well as catalase (CAT) and superoxide dismutase (SOD) activities were determined in renal tissues.

RESULTS

Gentamicin elevated the serum levels of creatinine, urea and uric acid as well as the MDA level in the renal tissue, while it decreased CAT, SOD activities and GSH levels as well as produced degenerative changes in glomeruli and tubules associated with increased expression of apoptotic markers and decreased expression of anti-apoptotic markers. Administration of cilostazol decreased urea, creatinine, uric acid and MDA levels while increased CAT and SOD activities and GSH levels as well as ameliorated the histopathological changes in relation to gentamicin group.

CONCLUSION

Cilostazol protected rats from gentamicin-induced nephrotoxicity possibly, in part through its antioxidant and anti-apoptotic activity.

Authors+Show Affiliations

Clinical Pharmacology Department, Faculty of Medicine, Zagazig University, Egypt.Clinical Pharmacology Department, Faculty of Medicine, Zagazig University, Egypt.Histology & Cell Biology department, Faculty of Medicine, Zagazig University, Egypt. Electronic address: mona_amer@rocketmail.com.Medical Biochemistry, Faculty of Medicine, Zagazig University, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26809659

Citation

Abdelsameea, Ahmed A., et al. "Cilostazol Attenuates Gentamicin-induced Nephrotoxicity in Rats." Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, vol. 68, no. 4, 2016, pp. 247-53.
Abdelsameea AA, Mohamed AM, Amer MG, et al. Cilostazol attenuates gentamicin-induced nephrotoxicity in rats. Exp Toxicol Pathol. 2016;68(4):247-53.
Abdelsameea, A. A., Mohamed, A. M., Amer, M. G., & Attia, S. M. (2016). Cilostazol attenuates gentamicin-induced nephrotoxicity in rats. Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, 68(4), 247-53. https://doi.org/10.1016/j.etp.2016.01.002
Abdelsameea AA, et al. Cilostazol Attenuates Gentamicin-induced Nephrotoxicity in Rats. Exp Toxicol Pathol. 2016;68(4):247-53. PubMed PMID: 26809659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cilostazol attenuates gentamicin-induced nephrotoxicity in rats. AU - Abdelsameea,Ahmed A, AU - Mohamed,Ahmed M, AU - Amer,Mona G, AU - Attia,Shahera M, Y1 - 2016/01/19/ PY - 2015/11/27/received PY - 2015/12/26/revised PY - 2016/01/07/accepted PY - 2016/1/27/entrez PY - 2016/1/27/pubmed PY - 2016/12/16/medline KW - Apoptosis KW - Cilostazol KW - Gentamicin KW - Nephrotoxicity KW - Ultrastructure SP - 247 EP - 53 JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JO - Exp Toxicol Pathol VL - 68 IS - 4 N2 - INTRODUCTION: Gentamycin is a widely used antibiotic. The nephrotoxic adverse effects of the drug may limit its use. Cilostazol, a phosphodiesterase III inhibitor, was reported to protect from renal oxidative stress. This work aimed to investigate the possible protective effect of cilostazol on gentamicin-induced nephrotoxicity and the possible underlying mechanisms. MATERIALS AND METHODS: 40 male albino rats were divided into 4 equal groups: (1) Control; (2) Cilostazol, 10mg/kg, p.o.; (3) Gentamicin, 80 mg/kg, i.p.; (4) Gentamicin 80 mg/kg, i.p. along with cilostazol 10mg/kg, p.o. All drugs were administered once daily for 8 days. On 9th day blood samples were collected for the estimation of creatinine, urea and uric acid in serum. Then the rats were sacrificed and kidneys were removed for light and electron microscope studies. Moreover, reduced glutathione (GSH) and malondialdehyde (MDA) levels as well as catalase (CAT) and superoxide dismutase (SOD) activities were determined in renal tissues. RESULTS: Gentamicin elevated the serum levels of creatinine, urea and uric acid as well as the MDA level in the renal tissue, while it decreased CAT, SOD activities and GSH levels as well as produced degenerative changes in glomeruli and tubules associated with increased expression of apoptotic markers and decreased expression of anti-apoptotic markers. Administration of cilostazol decreased urea, creatinine, uric acid and MDA levels while increased CAT and SOD activities and GSH levels as well as ameliorated the histopathological changes in relation to gentamicin group. CONCLUSION: Cilostazol protected rats from gentamicin-induced nephrotoxicity possibly, in part through its antioxidant and anti-apoptotic activity. SN - 1618-1433 UR - https://www.unboundmedicine.com/medline/citation/26809659/Cilostazol_attenuates_gentamicin_induced_nephrotoxicity_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0940-2993(16)30002-1 DB - PRIME DP - Unbound Medicine ER -