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Flavonoid and phenolic acid profile by LC-MS/MS and biological activity of crude extracts from Chenopodium hybridum aerial parts.
Nat Prod Res. 2016 Aug; 30(15):1766-70.NP

Abstract

Extracts from leaves and stems of Chenopodium hybridum were characterised for the presence and quantity of flavonoids and phenolic acids by LC-ESI-MS/MS. Five flavonoids and eight phenolic acids were detected for the first time in aerial parts of this plant species, the most abundant compounds being rutin (2.80 μg/g DW), 3-kaempferol rutinoside (2.91 μg/g DW), 4-OH-benzoic (1.86 μg/g DW) and syringic acids (2.31 μg/g DW). Extracts were tested for anti-inflammatory/antiarthritic, antihyaluronidase and cytotoxic activities against human prostate cancer (Du145, PC3) and melanoma cell lines (A375, HTB140 and WM793) of different malignancy. None of the extracts protected bovine serum albumin from heat-induced denaturation. Antihyaluronidase effect at the tested concentration was higher than standard naringenin. Cytotoxic activity was generally low with an exception of the extract from the leaves, which was found most effective against prostate Du145 cell line with 98.28 ± 1.13% of dead cells at 100 μg/mL.

Authors+Show Affiliations

a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.a Department of Pharmacognosy , Jagiellonian University Medical College , Cracow , Poland.c Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology , Jagiellonian University , Cracow , Poland.b Department of Pharmaceutical Botany , Medical University of Lublin , Lublin , Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26810568

Citation

Podolak, I, et al. "Flavonoid and Phenolic Acid Profile By LC-MS/MS and Biological Activity of Crude Extracts From Chenopodium Hybridum Aerial Parts." Natural Product Research, vol. 30, no. 15, 2016, pp. 1766-70.
Podolak I, Olech M, Galanty A, et al. Flavonoid and phenolic acid profile by LC-MS/MS and biological activity of crude extracts from Chenopodium hybridum aerial parts. Nat Prod Res. 2016;30(15):1766-70.
Podolak, I., Olech, M., Galanty, A., Załuski, D., Grabowska, K., Sobolewska, D., Michalik, M., & Nowak, R. (2016). Flavonoid and phenolic acid profile by LC-MS/MS and biological activity of crude extracts from Chenopodium hybridum aerial parts. Natural Product Research, 30(15), 1766-70. https://doi.org/10.1080/14786419.2015.1136908
Podolak I, et al. Flavonoid and Phenolic Acid Profile By LC-MS/MS and Biological Activity of Crude Extracts From Chenopodium Hybridum Aerial Parts. Nat Prod Res. 2016;30(15):1766-70. PubMed PMID: 26810568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonoid and phenolic acid profile by LC-MS/MS and biological activity of crude extracts from Chenopodium hybridum aerial parts. AU - Podolak,I, AU - Olech,M, AU - Galanty,A, AU - Załuski,D, AU - Grabowska,K, AU - Sobolewska,D, AU - Michalik,M, AU - Nowak,R, Y1 - 2016/01/25/ PY - 2016/1/27/entrez PY - 2016/1/27/pubmed PY - 2017/3/30/medline KW - Chenopodium hybridum KW - LC-MS/MS KW - antidenaturation activity KW - antihyaluronidase KW - cytotoxic activity KW - flavonoids KW - phenolic acids SP - 1766 EP - 70 JF - Natural product research JO - Nat Prod Res VL - 30 IS - 15 N2 - Extracts from leaves and stems of Chenopodium hybridum were characterised for the presence and quantity of flavonoids and phenolic acids by LC-ESI-MS/MS. Five flavonoids and eight phenolic acids were detected for the first time in aerial parts of this plant species, the most abundant compounds being rutin (2.80 μg/g DW), 3-kaempferol rutinoside (2.91 μg/g DW), 4-OH-benzoic (1.86 μg/g DW) and syringic acids (2.31 μg/g DW). Extracts were tested for anti-inflammatory/antiarthritic, antihyaluronidase and cytotoxic activities against human prostate cancer (Du145, PC3) and melanoma cell lines (A375, HTB140 and WM793) of different malignancy. None of the extracts protected bovine serum albumin from heat-induced denaturation. Antihyaluronidase effect at the tested concentration was higher than standard naringenin. Cytotoxic activity was generally low with an exception of the extract from the leaves, which was found most effective against prostate Du145 cell line with 98.28 ± 1.13% of dead cells at 100 μg/mL. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/26810568/Flavonoid_and_phenolic_acid_profile_by_LC_MS/MS_and_biological_activity_of_crude_extracts_from_Chenopodium_hybridum_aerial_parts_ L2 - https://www.tandfonline.com/doi/full/10.1080/14786419.2015.1136908 DB - PRIME DP - Unbound Medicine ER -