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Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species.
Mol Cytogenet. 2016; 9:6.MC

Abstract

BACKGROUND

The uncultivated species Dasypyrum breviaristatum carries novel diseases resistance and agronomically important genes of potential use for wheat improvement. The development of new wheat-D. breviaristatum derivatives lines with disease resistance provides an opportunity for the identification and localization of resistance genes on specific Dasypyrum chromosomes. The comparison of wheat-D. breviaristatum derivatives to the wheat-D. villosum derivatives enables to reveal the genomic divergence between D. breviaristatum and D. villosum.

RESULTS

The mitotic metaphase of the wheat- D. breviaristatum partial amphiploid TDH-2 and durum wheat -D. villosum amphiploid TDV-1 were studied using multicolor fluorescent in situ hybridization (FISH). We found that the distribution of FISH signals of telomeric, subtelomeric and centromeric regions on the D. breviaristatum chromosomes was different from those of D. villosum chromosomes by the probes of Oligo-pSc119.2, Oligo-pTa535, Oligo-(GAA)7 and Oligo-pHv62-1. A wheat line D2139, selected from a cross between wheat lines MY11 and TDH-2, was characterized by FISH and PCR-based molecular markers. FISH analysis demonstrated that D2139 contained 44 chromosomes including a pair of D. breviaristatum chromosomes which had originated from the partial amphiploid TDH-2. Molecular markers confirmed that the introduced D. breviaristatum chromosomes belonged to homoeologous group 7, indicating that D2139 was a 7V(b) disomic addition line. The D2139 displayed high resistance to wheat stripe rust races at adult stage plant, which may be inherited from, D. breviaristatum chromosome 7V(b).

CONCLUSION

The study present here revealed that the large divergence between D. breviaristatum and D. villosum with respected to the organization of different repetitive sequences. The identified wheat- D. breviaristatum chromosome addition line D2139 will be used to produce agronomically desirable germplasm for wheat breeding.

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054 China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26813790

Citation

Li, Guangrong, et al. "Molecular Cytogenetic Characterization of Dasypyrum Breviaristatum Chromosomes in Wheat Background Revealing the Genomic Divergence Between Dasypyrum Species." Molecular Cytogenetics, vol. 9, 2016, p. 6.
Li G, Gao D, Zhang H, et al. Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. Mol Cytogenet. 2016;9:6.
Li, G., Gao, D., Zhang, H., Li, J., Wang, H., La, S., Ma, J., & Yang, Z. (2016). Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. Molecular Cytogenetics, 9, 6. https://doi.org/10.1186/s13039-016-0217-0
Li G, et al. Molecular Cytogenetic Characterization of Dasypyrum Breviaristatum Chromosomes in Wheat Background Revealing the Genomic Divergence Between Dasypyrum Species. Mol Cytogenet. 2016;9:6. PubMed PMID: 26813790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. AU - Li,Guangrong, AU - Gao,Dan, AU - Zhang,Hongjun, AU - Li,Jianbo, AU - Wang,Hongjin, AU - La,Shixiao, AU - Ma,Jiwei, AU - Yang,Zujun, Y1 - 2016/01/25/ PY - 2015/12/03/received PY - 2016/01/19/accepted PY - 2016/1/28/entrez PY - 2016/1/28/pubmed PY - 2016/1/28/medline KW - Dasypyrum breviaristatum KW - Fluorescence in situ hybridization KW - Molecular marker KW - Wheat SP - 6 EP - 6 JF - Molecular cytogenetics JO - Mol Cytogenet VL - 9 N2 - BACKGROUND: The uncultivated species Dasypyrum breviaristatum carries novel diseases resistance and agronomically important genes of potential use for wheat improvement. The development of new wheat-D. breviaristatum derivatives lines with disease resistance provides an opportunity for the identification and localization of resistance genes on specific Dasypyrum chromosomes. The comparison of wheat-D. breviaristatum derivatives to the wheat-D. villosum derivatives enables to reveal the genomic divergence between D. breviaristatum and D. villosum. RESULTS: The mitotic metaphase of the wheat- D. breviaristatum partial amphiploid TDH-2 and durum wheat -D. villosum amphiploid TDV-1 were studied using multicolor fluorescent in situ hybridization (FISH). We found that the distribution of FISH signals of telomeric, subtelomeric and centromeric regions on the D. breviaristatum chromosomes was different from those of D. villosum chromosomes by the probes of Oligo-pSc119.2, Oligo-pTa535, Oligo-(GAA)7 and Oligo-pHv62-1. A wheat line D2139, selected from a cross between wheat lines MY11 and TDH-2, was characterized by FISH and PCR-based molecular markers. FISH analysis demonstrated that D2139 contained 44 chromosomes including a pair of D. breviaristatum chromosomes which had originated from the partial amphiploid TDH-2. Molecular markers confirmed that the introduced D. breviaristatum chromosomes belonged to homoeologous group 7, indicating that D2139 was a 7V(b) disomic addition line. The D2139 displayed high resistance to wheat stripe rust races at adult stage plant, which may be inherited from, D. breviaristatum chromosome 7V(b). CONCLUSION: The study present here revealed that the large divergence between D. breviaristatum and D. villosum with respected to the organization of different repetitive sequences. The identified wheat- D. breviaristatum chromosome addition line D2139 will be used to produce agronomically desirable germplasm for wheat breeding. SN - 1755-8166 UR - https://www.unboundmedicine.com/medline/citation/26813790/Molecular_cytogenetic_characterization_of_Dasypyrum_breviaristatum_chromosomes_in_wheat_background_revealing_the_genomic_divergence_between_Dasypyrum_species_ L2 - https://molecularcytogenetics.biomedcentral.com/articles/10.1186/s13039-016-0217-0 DB - PRIME DP - Unbound Medicine ER -
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