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Store-operated Ca2+ Entry-associated Regulatory factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels.
J Biol Chem. 2016 Mar 25; 291(13):6982-8.JB

Abstract

The store-operated Ca(2+)entry-associated regulatory factor (SARAF) has recently been identified as a STIM1 regulatory protein that facilitates slow Ca(2+)-dependent inactivation of store-operated Ca(2+)entry (SOCE). Both the store-operated channels and the store-independent arachidonate-regulated Ca(2+)(ARC) channels are regulated by STIM1. In the present study, we show that, in addition to its location in the endoplasmic reticulum, SARAF is constitutively expressed in the plasma membrane, where it can interact with plasma membrane (PM)-resident ARC forming subunits in the neuroblastoma cell line SH-SY5Y. Using siRNA-based and overexpression approaches we report that SARAF negatively regulates store-independent Ca(2+)entry via the ARC channels. Arachidonic acid (AA) increases the association of PM-resident SARAF with Orai1. Finally, our results indicate that SARAF modulates the ability of AA to promote cell survival in neuroblastoma cells. In addition to revealing new insight into the biology of ARC channels in neuroblastoma cells, these findings provide evidence for an unprecedented location of SARAF in the plasma membrane.

Authors+Show Affiliations

From the Department of Physiology (Cellular Physiology Research Group), University of Extremadura, 10003 Caceres, Spain and.From the Department of Physiology (Cellular Physiology Research Group), University of Extremadura, 10003 Caceres, Spain and.Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland.From the Department of Physiology (Cellular Physiology Research Group), University of Extremadura, 10003 Caceres, Spain and.From the Department of Physiology (Cellular Physiology Research Group), University of Extremadura, 10003 Caceres, Spain and jarosado@unex.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26817842

Citation

Albarran, Letizia, et al. "Store-operated Ca2+ Entry-associated Regulatory Factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels." The Journal of Biological Chemistry, vol. 291, no. 13, 2016, pp. 6982-8.
Albarran L, Lopez JJ, Woodard GE, et al. Store-operated Ca2+ Entry-associated Regulatory factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels. J Biol Chem. 2016;291(13):6982-8.
Albarran, L., Lopez, J. J., Woodard, G. E., Salido, G. M., & Rosado, J. A. (2016). Store-operated Ca2+ Entry-associated Regulatory factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels. The Journal of Biological Chemistry, 291(13), 6982-8. https://doi.org/10.1074/jbc.M115.704940
Albarran L, et al. Store-operated Ca2+ Entry-associated Regulatory Factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels. J Biol Chem. 2016 Mar 25;291(13):6982-8. PubMed PMID: 26817842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Store-operated Ca2+ Entry-associated Regulatory factor (SARAF) Plays an Important Role in the Regulation of Arachidonate-regulated Ca2+ (ARC) Channels. AU - Albarran,Letizia, AU - Lopez,Jose J, AU - Woodard,Geoffrey E, AU - Salido,Gines M, AU - Rosado,Juan A, Y1 - 2016/01/27/ PY - 2015/11/17/received PY - 2016/1/29/entrez PY - 2016/1/29/pubmed PY - 2016/8/5/medline KW - ARC channels KW - SARAF KW - arachidonic acid (AA) (ARA) KW - calcium channel KW - calcium release-activated calcium channel protein 1 (ORAI1) KW - protein-protein interaction KW - stromal interaction molecule 1 (STIM1) SP - 6982 EP - 8 JF - The Journal of biological chemistry JO - J Biol Chem VL - 291 IS - 13 N2 - The store-operated Ca(2+)entry-associated regulatory factor (SARAF) has recently been identified as a STIM1 regulatory protein that facilitates slow Ca(2+)-dependent inactivation of store-operated Ca(2+)entry (SOCE). Both the store-operated channels and the store-independent arachidonate-regulated Ca(2+)(ARC) channels are regulated by STIM1. In the present study, we show that, in addition to its location in the endoplasmic reticulum, SARAF is constitutively expressed in the plasma membrane, where it can interact with plasma membrane (PM)-resident ARC forming subunits in the neuroblastoma cell line SH-SY5Y. Using siRNA-based and overexpression approaches we report that SARAF negatively regulates store-independent Ca(2+)entry via the ARC channels. Arachidonic acid (AA) increases the association of PM-resident SARAF with Orai1. Finally, our results indicate that SARAF modulates the ability of AA to promote cell survival in neuroblastoma cells. In addition to revealing new insight into the biology of ARC channels in neuroblastoma cells, these findings provide evidence for an unprecedented location of SARAF in the plasma membrane. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/26817842/Store_operated_Ca2+_Entry_associated_Regulatory_factor__SARAF__Plays_an_Important_Role_in_the_Regulation_of_Arachidonate_regulated_Ca2+__ARC__Channels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/M115.704940 DB - PRIME DP - Unbound Medicine ER -