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The aberrantly expressed miR-193b-3p contributes to preeclampsia through regulating transforming growth factor-β signaling.
Sci Rep 2016; 6:19910SR

Abstract

Preeclampsia (PE) is a leading cause of maternal mortality worldwide. Several studies have detected some differentially expressed microRNAs in the preeclamptic placenta, but few of the identified microRNAs demonstrated consistent findings among different research studies. In this study, high-throughput microRNA sequencing (HTS) of 9 preeclamptic and 9 normal placentas was performed. Seventeen microRNAs were identified to be up-regulated, and 8 down-regulated in preeclamptic placentas. Eight differentially expressed microRNAs except one identified in our study were determined to be consistent with at least one previous study, while sixteen were newly found. We performed qRT-PCR with independent 22 preeclamptic placentas and 20 control placentas to verify the differentially expressed microRNAs, and ten microRNAs were validated. The predicted target genes of the aberrantly expressed miR-193b-3p were enriched in the following gene ontology categories: cell motility and migration, cell proliferation and angiogenesis. We also found that miR-193b-3p significantly decreased the migration and invasion of trophoblast (HTR-8/SVneo) cells and that miR-193b-3p could regulate trophoblasts migration and invasion through binding onto the 3'UTR target site of TGF-β2. In conclusion, we identified a list of differentially expressed microRNAs in PE placentas by HTS and provided preliminary evidence for the role of miR-193b-3p in the pathogenesis of preeclampsia.

Authors+Show Affiliations

Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Department of Obstetrics, Provincial Hospital Affiliated to Shandong University, Jinan, China.Departments of Pathology and Biobank, International Peace Maternity &Child Health Hospital of China affiliated to Shanghai Jiao Tong University, Shanghai, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.Children's Hospital and Institutes of Biomedical Sciences, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26822621

Citation

Zhou, Xinyao, et al. "The Aberrantly Expressed miR-193b-3p Contributes to Preeclampsia Through Regulating Transforming Growth Factor-β Signaling." Scientific Reports, vol. 6, 2016, p. 19910.
Zhou X, Li Q, Xu J, et al. The aberrantly expressed miR-193b-3p contributes to preeclampsia through regulating transforming growth factor-β signaling. Sci Rep. 2016;6:19910.
Zhou, X., Li, Q., Xu, J., Zhang, X., Zhang, H., Xiang, Y., ... Zhao, X. (2016). The aberrantly expressed miR-193b-3p contributes to preeclampsia through regulating transforming growth factor-β signaling. Scientific Reports, 6, p. 19910. doi:10.1038/srep19910.
Zhou X, et al. The Aberrantly Expressed miR-193b-3p Contributes to Preeclampsia Through Regulating Transforming Growth Factor-β Signaling. Sci Rep. 2016 Jan 29;6:19910. PubMed PMID: 26822621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The aberrantly expressed miR-193b-3p contributes to preeclampsia through regulating transforming growth factor-β signaling. AU - Zhou,Xinyao, AU - Li,Qiaoli, AU - Xu,Jiawei, AU - Zhang,Xiaojing, AU - Zhang,Huijuan, AU - Xiang,Yuqian, AU - Fang,Chuantao, AU - Wang,Teng, AU - Xia,Shihui, AU - Zhang,Qiang, AU - Xing,Qinghe, AU - He,Lin, AU - Wang,Lei, AU - Xu,Mingqing, AU - Zhao,Xinzhi, Y1 - 2016/01/29/ PY - 2015/06/24/received PY - 2015/12/21/accepted PY - 2016/1/30/entrez PY - 2016/1/30/pubmed PY - 2017/1/7/medline SP - 19910 EP - 19910 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Preeclampsia (PE) is a leading cause of maternal mortality worldwide. Several studies have detected some differentially expressed microRNAs in the preeclamptic placenta, but few of the identified microRNAs demonstrated consistent findings among different research studies. In this study, high-throughput microRNA sequencing (HTS) of 9 preeclamptic and 9 normal placentas was performed. Seventeen microRNAs were identified to be up-regulated, and 8 down-regulated in preeclamptic placentas. Eight differentially expressed microRNAs except one identified in our study were determined to be consistent with at least one previous study, while sixteen were newly found. We performed qRT-PCR with independent 22 preeclamptic placentas and 20 control placentas to verify the differentially expressed microRNAs, and ten microRNAs were validated. The predicted target genes of the aberrantly expressed miR-193b-3p were enriched in the following gene ontology categories: cell motility and migration, cell proliferation and angiogenesis. We also found that miR-193b-3p significantly decreased the migration and invasion of trophoblast (HTR-8/SVneo) cells and that miR-193b-3p could regulate trophoblasts migration and invasion through binding onto the 3'UTR target site of TGF-β2. In conclusion, we identified a list of differentially expressed microRNAs in PE placentas by HTS and provided preliminary evidence for the role of miR-193b-3p in the pathogenesis of preeclampsia. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26822621/The_aberrantly_expressed_miR_193b_3p_contributes_to_preeclampsia_through_regulating_transforming_growth_factor_β_signaling_ L2 - http://dx.doi.org/10.1038/srep19910 DB - PRIME DP - Unbound Medicine ER -