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Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model.
Mol Immunol 2016; 71:34-41MI

Abstract

In this study, the immunogenicity and protective efficacy of recombinant proteins Omp19 (rO) and P39 (rP) from Brucella abortus were evaluated individually and compared with the cocktail protein (rO+rP) against B. abortus 544 and Brucella melitensis 16M infection in BALB/c mouse model. Intra-peritoneal (I.P.) immunization with rO+rP cocktail developed substantially higher antibody titers predominant with Th1 mediated isotypes (IgG2a/2b). Western blot analysis using anti-rO+rP antibodies showed specific reactivity with native Omp19 (19 kDa) and P39 (39 kDa) among whole cell proteins of B. abortus and B. melitensis. Splenocytes extracted from rO+rP immunized mice induced significantly (P<0.001) higher proliferative responses at 30 μg/ml with considerable expression of pro-inflammatory cytokines (IFN-γ, IL-2 and IL-12) than rO and rP. Macrophage cell (RAW 264.7) monolayer supplemented with anti-rO+rP polysera exhibited enhanced viability against challenge with B. abortus 544 (72.27%) and B. melitensis 16M (68.57%). On the other hand, individual anti-rO and anti-rP polysera resulted in relatively lesser protection against the pathogens (64.79%, 54.45% and 47.13%, 45.11%, respectively). Immunized group of mice when I.P. challenged with 5 × 10(4) CFU of B. abortus 544 and B. melitensis 16M were found significantly (P<0.001) protected in the rO+rP group (log units of protection, spleen: 2.38, 2.12; liver: 1.04, 0.81, respectively) than in rO (spleen: 1.43, 1.21; liver: 0.7, 0.47) and rP (spleen: 1.24, 1.17; liver: 0.65, 0.34). Findings from this study depicted that rO+rP cocktail is highly immunogenic with the Th1 predominant serum antibody titers and T-cell mediated immune protection, would be a valuable intervention in the development of a safer and improved Brucella vaccine.

Authors+Show Affiliations

Department of Microbiology, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India. Electronic address: ganeshbabutmln@gmail.com.Department of Microbiology, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India.Department of Microbiology, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India.Department of Microbiology, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India.Department of Microbiology, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India. Electronic address: drharshvardhanbatra@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26826463

Citation

Tadepalli, Ganesh, et al. "Immunogenicity and Protective Efficacy of Brucella Abortus Recombinant Protein Cocktail (rOmp19+rP39) Against B. Abortus 544 and B. Melitensis 16M Infection in Murine Model." Molecular Immunology, vol. 71, 2016, pp. 34-41.
Tadepalli G, Singh AK, Balakrishna K, et al. Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model. Mol Immunol. 2016;71:34-41.
Tadepalli, G., Singh, A. K., Balakrishna, K., Murali, H. S., & Batra, H. V. (2016). Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model. Molecular Immunology, 71, pp. 34-41. doi:10.1016/j.molimm.2016.01.001.
Tadepalli G, et al. Immunogenicity and Protective Efficacy of Brucella Abortus Recombinant Protein Cocktail (rOmp19+rP39) Against B. Abortus 544 and B. Melitensis 16M Infection in Murine Model. Mol Immunol. 2016;71:34-41. PubMed PMID: 26826463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model. AU - Tadepalli,Ganesh, AU - Singh,Amit Kumar, AU - Balakrishna,Konduru, AU - Murali,Harishchandra Sripathy, AU - Batra,Harsh Vardhan, Y1 - 2016/01/27/ PY - 2015/08/24/received PY - 2015/12/30/revised PY - 2016/01/04/accepted PY - 2016/1/31/entrez PY - 2016/1/31/pubmed PY - 2016/8/3/medline KW - B. abortus KW - B. melitensis KW - Omp19 KW - P39 KW - Protein cocktail (rO+rP) SP - 34 EP - 41 JF - Molecular immunology JO - Mol. Immunol. VL - 71 N2 - In this study, the immunogenicity and protective efficacy of recombinant proteins Omp19 (rO) and P39 (rP) from Brucella abortus were evaluated individually and compared with the cocktail protein (rO+rP) against B. abortus 544 and Brucella melitensis 16M infection in BALB/c mouse model. Intra-peritoneal (I.P.) immunization with rO+rP cocktail developed substantially higher antibody titers predominant with Th1 mediated isotypes (IgG2a/2b). Western blot analysis using anti-rO+rP antibodies showed specific reactivity with native Omp19 (19 kDa) and P39 (39 kDa) among whole cell proteins of B. abortus and B. melitensis. Splenocytes extracted from rO+rP immunized mice induced significantly (P<0.001) higher proliferative responses at 30 μg/ml with considerable expression of pro-inflammatory cytokines (IFN-γ, IL-2 and IL-12) than rO and rP. Macrophage cell (RAW 264.7) monolayer supplemented with anti-rO+rP polysera exhibited enhanced viability against challenge with B. abortus 544 (72.27%) and B. melitensis 16M (68.57%). On the other hand, individual anti-rO and anti-rP polysera resulted in relatively lesser protection against the pathogens (64.79%, 54.45% and 47.13%, 45.11%, respectively). Immunized group of mice when I.P. challenged with 5 × 10(4) CFU of B. abortus 544 and B. melitensis 16M were found significantly (P<0.001) protected in the rO+rP group (log units of protection, spleen: 2.38, 2.12; liver: 1.04, 0.81, respectively) than in rO (spleen: 1.43, 1.21; liver: 0.7, 0.47) and rP (spleen: 1.24, 1.17; liver: 0.65, 0.34). Findings from this study depicted that rO+rP cocktail is highly immunogenic with the Th1 predominant serum antibody titers and T-cell mediated immune protection, would be a valuable intervention in the development of a safer and improved Brucella vaccine. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/26826463/Immunogenicity_and_protective_efficacy_of_Brucella_abortus_recombinant_protein_cocktail__rOmp19+rP39__against_B__abortus_544_and_B__melitensis_16M_infection_in_murine_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(16)30002-5 DB - PRIME DP - Unbound Medicine ER -