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Correlation of Clinical Outcomes with Quantitative Polymerase Chain Reaction DNA Copy Number in Patients with Acute Retinal Necrosis.
Ocul Immunol Inflamm. 2017 Apr; 25(2):246-252.OI

Abstract

PURPOSE

To correlate visual acuity outcomes and clinical features with quantitative PCR DNA copy number in patients with acute retinal necrosis (ARN).

METHODS

Retrospective, consecutive case series.

RESULTS

In total, 14 eyes of 13 patients were diagnosed with ARN, based on the American Uveitis Society criteria, and were followed for a mean of 324.5 days (median 250.5 days, SD ± 214 days). Anterior chamber fluid analyzed by quantitative PCR identified viral DNA in 11 of 14 eyes (78.5%). Varicella zoster virus (VZV) was identified in seven eyes (50%) and herpes simplex virus (HSV) in four eyes (28.5%). Mean DNA copy number was 7.9 × 106/mL (median 2.10 × 106/mL, range: 0-5.60 × 107/mL). Eyes with quantitative PCR DNA copy number of ≥5.0 × 106/mL (n = 6 eyes) had worse baseline visual acuity (logMAR 1.48 ± 0.71 vs 0.94 ± 0.76, p = 0.196) and final visual acuity (logMAR 2.10 ± 0.60 vs 0.82 ± 0.81, p = 0.007) compared with patients with a DNA copy number <5.0 × 106/mL (n = 8 eyes). Patients with a DNA copy number of ≥5.0 × 106/mL were more likely to have at least 5 clock hours of retinitis on funduscopic exam (p = 0.03) and developed retinal detachment more frequently (p = 0.08).

CONCLUSIONS

Quantitative DNA copy number of ≥5.0 × 106/mL is associated with more extensive retinitis, worse visual acuity, and development of retinal detachment in patients with acute retinal necrosis.

Authors+Show Affiliations

a Uveitis Unit, The Retina Service of Wills Eye Hospital , Philadelphia , Pennsylvania , USA.a Uveitis Unit, The Retina Service of Wills Eye Hospital , Philadelphia , Pennsylvania , USA. b Mid-Atlantic Retina , Philadelphia , Pennsylvania , USA.a Uveitis Unit, The Retina Service of Wills Eye Hospital , Philadelphia , Pennsylvania , USA. b Mid-Atlantic Retina , Philadelphia , Pennsylvania , USA.a Uveitis Unit, The Retina Service of Wills Eye Hospital , Philadelphia , Pennsylvania , USA. b Mid-Atlantic Retina , Philadelphia , Pennsylvania , USA.a Uveitis Unit, The Retina Service of Wills Eye Hospital , Philadelphia , Pennsylvania , USA. b Mid-Atlantic Retina , Philadelphia , Pennsylvania , USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26828388

Citation

Calvo, Charles M., et al. "Correlation of Clinical Outcomes With Quantitative Polymerase Chain Reaction DNA Copy Number in Patients With Acute Retinal Necrosis." Ocular Immunology and Inflammation, vol. 25, no. 2, 2017, pp. 246-252.
Calvo CM, Khan MA, Mehta S, et al. Correlation of Clinical Outcomes with Quantitative Polymerase Chain Reaction DNA Copy Number in Patients with Acute Retinal Necrosis. Ocul Immunol Inflamm. 2017;25(2):246-252.
Calvo, C. M., Khan, M. A., Mehta, S., Garg, S. J., & Dunn, J. P. (2017). Correlation of Clinical Outcomes with Quantitative Polymerase Chain Reaction DNA Copy Number in Patients with Acute Retinal Necrosis. Ocular Immunology and Inflammation, 25(2), 246-252. https://doi.org/10.3109/09273948.2015.1115081
Calvo CM, et al. Correlation of Clinical Outcomes With Quantitative Polymerase Chain Reaction DNA Copy Number in Patients With Acute Retinal Necrosis. Ocul Immunol Inflamm. 2017;25(2):246-252. PubMed PMID: 26828388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation of Clinical Outcomes with Quantitative Polymerase Chain Reaction DNA Copy Number in Patients with Acute Retinal Necrosis. AU - Calvo,Charles M, AU - Khan,Mohammed Ali, AU - Mehta,Sonia, AU - Garg,Sunir J, AU - Dunn,James P, Y1 - 2016/01/30/ PY - 2016/2/2/pubmed PY - 2018/1/9/medline PY - 2016/2/2/entrez KW - Acute retinal necrosis KW - herpes simplex virus KW - necrotizing retinitis KW - polymerase chain reaction KW - retinitis SP - 246 EP - 252 JF - Ocular immunology and inflammation JO - Ocul. Immunol. Inflamm. VL - 25 IS - 2 N2 - PURPOSE: To correlate visual acuity outcomes and clinical features with quantitative PCR DNA copy number in patients with acute retinal necrosis (ARN). METHODS: Retrospective, consecutive case series. RESULTS: In total, 14 eyes of 13 patients were diagnosed with ARN, based on the American Uveitis Society criteria, and were followed for a mean of 324.5 days (median 250.5 days, SD ± 214 days). Anterior chamber fluid analyzed by quantitative PCR identified viral DNA in 11 of 14 eyes (78.5%). Varicella zoster virus (VZV) was identified in seven eyes (50%) and herpes simplex virus (HSV) in four eyes (28.5%). Mean DNA copy number was 7.9 × 106/mL (median 2.10 × 106/mL, range: 0-5.60 × 107/mL). Eyes with quantitative PCR DNA copy number of ≥5.0 × 106/mL (n = 6 eyes) had worse baseline visual acuity (logMAR 1.48 ± 0.71 vs 0.94 ± 0.76, p = 0.196) and final visual acuity (logMAR 2.10 ± 0.60 vs 0.82 ± 0.81, p = 0.007) compared with patients with a DNA copy number <5.0 × 106/mL (n = 8 eyes). Patients with a DNA copy number of ≥5.0 × 106/mL were more likely to have at least 5 clock hours of retinitis on funduscopic exam (p = 0.03) and developed retinal detachment more frequently (p = 0.08). CONCLUSIONS: Quantitative DNA copy number of ≥5.0 × 106/mL is associated with more extensive retinitis, worse visual acuity, and development of retinal detachment in patients with acute retinal necrosis. SN - 1744-5078 UR - https://www.unboundmedicine.com/medline/citation/26828388/Correlation_of_Clinical_Outcomes_with_Quantitative_Polymerase_Chain_Reaction_DNA_Copy_Number_in_Patients_with_Acute_Retinal_Necrosis_ L2 - http://www.tandfonline.com/doi/full/10.3109/09273948.2015.1115081 DB - PRIME DP - Unbound Medicine ER -