Tags

Type your tag names separated by a space and hit enter

Evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations.
Int J Cosmet Sci 2016; 38(5):535-40IJ

Abstract

BACKGROUND

Bromelain is a mixture of proteolytic enzymes found in various tissues of the pineapple plant (Ananas comosus) and other species of Bromeliaceae. Owing to its proteolytic activity, bromelain has been used in the food, medical, pharmaceutical and cosmetic industries, for its cell renewal, anti-ageing, whitening and anti-cellulite properties.

OBJECTIVE

This study evaluated the stability of bromelain (commercial powder) incorporated in topical formulations.

METHODS

Bromelain was incorporated at three concentrations, 0.5%, 1.0% and 2.0%, in oil-in-water emulsion and gel, and stored for six months at varying stress conditions. Stability was accessed by measuring the changes in the protein content, enzymatic activity, viscosity, rheology, pH and colour of the selected formulations.

RESULTS

The colour of all the samples changed after 180 days of incubation, indicating the concentration-dependence and temperature-sensitive nature of these formulations. No relationship was observed between the changes in the pH, temperature and luminosity exposure in all the samples. Gels proved to be the least preferred base for incorporation of bromelain for use as a topical formulation, owing to its inability to maintain the integrity of bromelain, thereby affecting the formulation characteristics.

CONCLUSION

The emulsion-based formulations at all the concentrations of bromelain were more stable than the gel-based formulation over 180 days of evaluation, at a temperature of 5°C, protected from light.

Authors+Show Affiliations

Postgraduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.Postgraduate Program in Biosciences and Technology of Bioactive Products, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.Posdoctorade Program in Medical Sciences, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.Posdoctorade Program in Medical Sciences, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil. Genetics and Biochesmitry Institute, Uberlandia Federal University (UFU), Uberlandia, Brazil.School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, Brazil.Faculty of Pharmaceutical Sciences, University of Campinas (UNICAMP), Campinas, Brazil. pmazzola@fcf.unicamp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26833020

Citation

Lourenço, C B., et al. "Evaluation of the Enzymatic Activity and Stability of Commercial Bromelain Incorporated in Topical Formulations." International Journal of Cosmetic Science, vol. 38, no. 5, 2016, pp. 535-40.
Lourenço CB, Ataide JA, Cefali LC, et al. Evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations. Int J Cosmet Sci. 2016;38(5):535-40.
Lourenço, C. B., Ataide, J. A., Cefali, L. C., Novaes, L. C., Moriel, P., Silveira, E., ... Mazzola, P. G. (2016). Evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations. International Journal of Cosmetic Science, 38(5), pp. 535-40. doi:10.1111/ics.12308.
Lourenço CB, et al. Evaluation of the Enzymatic Activity and Stability of Commercial Bromelain Incorporated in Topical Formulations. Int J Cosmet Sci. 2016;38(5):535-40. PubMed PMID: 26833020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the enzymatic activity and stability of commercial bromelain incorporated in topical formulations. AU - Lourenço,C B, AU - Ataide,J A, AU - Cefali,L C, AU - Novaes,L C D L, AU - Moriel,P, AU - Silveira,E, AU - Tambourgi,E B, AU - Mazzola,P G, Y1 - 2016/03/16/ PY - 2015/12/23/received PY - 2016/01/22/accepted PY - 2016/2/3/entrez PY - 2016/2/3/pubmed PY - 2017/4/22/medline KW - bromelain KW - cosmetics KW - emulsions KW - enzymatic activity KW - formulation KW - gels KW - stability SP - 535 EP - 40 JF - International journal of cosmetic science JO - Int J Cosmet Sci VL - 38 IS - 5 N2 - BACKGROUND: Bromelain is a mixture of proteolytic enzymes found in various tissues of the pineapple plant (Ananas comosus) and other species of Bromeliaceae. Owing to its proteolytic activity, bromelain has been used in the food, medical, pharmaceutical and cosmetic industries, for its cell renewal, anti-ageing, whitening and anti-cellulite properties. OBJECTIVE: This study evaluated the stability of bromelain (commercial powder) incorporated in topical formulations. METHODS: Bromelain was incorporated at three concentrations, 0.5%, 1.0% and 2.0%, in oil-in-water emulsion and gel, and stored for six months at varying stress conditions. Stability was accessed by measuring the changes in the protein content, enzymatic activity, viscosity, rheology, pH and colour of the selected formulations. RESULTS: The colour of all the samples changed after 180 days of incubation, indicating the concentration-dependence and temperature-sensitive nature of these formulations. No relationship was observed between the changes in the pH, temperature and luminosity exposure in all the samples. Gels proved to be the least preferred base for incorporation of bromelain for use as a topical formulation, owing to its inability to maintain the integrity of bromelain, thereby affecting the formulation characteristics. CONCLUSION: The emulsion-based formulations at all the concentrations of bromelain were more stable than the gel-based formulation over 180 days of evaluation, at a temperature of 5°C, protected from light. SN - 1468-2494 UR - https://www.unboundmedicine.com/medline/citation/26833020/Evaluation_of_the_enzymatic_activity_and_stability_of_commercial_bromelain_incorporated_in_topical_formulations_ L2 - https://doi.org/10.1111/ics.12308 DB - PRIME DP - Unbound Medicine ER -