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Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress.
Sci Rep. 2016 Feb 02; 6:20245.SR

Abstract

Arbuscular mycorrhizal fungi (AMF) protect host plants against diverse biotic and abiotic stresses, and promote biodegradation of various contaminants. In this study effect of Glomus mosseae/Medicago sativa mycorrhiza on atrazine degradation was investigated. It was observed that the atrazine degradation rates with any addition level in mycorrhizal treatments were all significantly higher than those in non-mycorrhizal treatments. When atrazine was applied at 20 mg kg(-1), the removal efficiency was up to 74.65%. Therefore, G. mosseae can be considered as ideal inhabitants of technical installations to facilitate phytoremediation. Furthermore, a total of 10.4 Gb was used for de novo transcriptome assembly, resulting in a comprehensive data set for the identification of genes corresponding to atrazine stress in the AM association. After comparative analysis with edgeR, a total of 2,060 differential expressed genes were identified, including 570 up-regulated genes and 1490 down-regulated genes. After excluding 'function unknown' and 'general function predictions only' genes, 172 up-regulated genes were obtained. The differentially expressed genes in AM association with and without atrazine stress were associated with molecular processes/other proteins, zinc finger protein, intracellular/extracellular enzymes, structural proteins, anti-stress/anti-disease protein, electron transport-related protein, and plant growth associated protein. Our results not only prove AMF has important ecological significance on atrazine degradation but also provide evidence for the molecular mechanisms of atrazine degradation by AMF.

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Authors+Show Affiliations

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

Department of Biology, Washington University at St Louis, Saint Louis, 1 Brookings Drive MO 63130, USA.

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

Bio-ecological Restoration, School of Life Science, Heilongjiang University, Harbin, 74 Xuefu Road Heilongjiang 150080, China.

MeSH

AtrazineComputational BiologyGene Expression ProfilingGene Expression Regulation, PlantGene OntologyGlomeromycotaHerbicidesMedicago sativaMolecular Sequence AnnotationMycorrhizaePlant RootsReproducibility of ResultsStress, PhysiologicalTranscriptome

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26833403

Citation

Song, Fuqiang, et al. "Transcriptome Analysis of Glomus mosseae/Medicago Sativa Mycorrhiza On Atrazine Stress." Scientific Reports, vol. 6, 2016, p. 20245.

Song F, Li J, Fan X, et al. Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress. Sci Rep. 2016;6:20245.

Song, F., Li, J., Fan, X., Zhang, Q., Chang, W., Yang, F., & Geng, G. (2016). Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress. Scientific Reports, 6, 20245. https://doi.org/10.1038/srep20245

Song F, et al. Transcriptome Analysis of Glomus mosseae/Medicago Sativa Mycorrhiza On Atrazine Stress. Sci Rep. 2016 Feb 2;6:20245. PubMed PMID: 26833403.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transcriptome analysis of Glomus mosseae/Medicago sativa mycorrhiza on atrazine stress. AU - Song,Fuqiang, AU - Li,Jize, AU - Fan,Xiaoxu, AU - Zhang,Quan, AU - Chang,Wei, AU - Yang,Fengshan, AU - Geng,Gui, Y1 - 2016/02/02/ PY - 2015/04/20/received PY - 2015/12/30/accepted PY - 2016/2/3/entrez PY - 2016/2/3/pubmed PY - 2017/1/7/medline SP - 20245 EP - 20245 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Arbuscular mycorrhizal fungi (AMF) protect host plants against diverse biotic and abiotic stresses, and promote biodegradation of various contaminants. In this study effect of Glomus mosseae/Medicago sativa mycorrhiza on atrazine degradation was investigated. It was observed that the atrazine degradation rates with any addition level in mycorrhizal treatments were all significantly higher than those in non-mycorrhizal treatments. When atrazine was applied at 20 mg kg(-1), the removal efficiency was up to 74.65%. Therefore, G. mosseae can be considered as ideal inhabitants of technical installations to facilitate phytoremediation. Furthermore, a total of 10.4 Gb was used for de novo transcriptome assembly, resulting in a comprehensive data set for the identification of genes corresponding to atrazine stress in the AM association. After comparative analysis with edgeR, a total of 2,060 differential expressed genes were identified, including 570 up-regulated genes and 1490 down-regulated genes. After excluding 'function unknown' and 'general function predictions only' genes, 172 up-regulated genes were obtained. The differentially expressed genes in AM association with and without atrazine stress were associated with molecular processes/other proteins, zinc finger protein, intracellular/extracellular enzymes, structural proteins, anti-stress/anti-disease protein, electron transport-related protein, and plant growth associated protein. Our results not only prove AMF has important ecological significance on atrazine degradation but also provide evidence for the molecular mechanisms of atrazine degradation by AMF. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/26833403/Transcriptome_analysis_of_Glomus_mosseae/Medicago_sativa_mycorrhiza_on_atrazine_stress_ DB - PRIME DP - Unbound Medicine ER -