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[An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study].
Yao Xue Xue Bao 2015; 50(10):1330-5YX

Abstract

ABSTRACT

The study aims to develop an LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in urine samples, and to investigate their urinary excretion characteristics in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection (HTLPI). The urine samples were extracted by methanol, and then separated on a Hedera ODS-2 column with a mobile phase of acetonitrile and 5 mmol · L(-1) ammonium acetate buffer solution containing 0.05% formic acid (20:80). Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited good linearity over the concentration range of 0.03 -40 µg · mL(-1). The values on both the occasions (intra- and inter-day) were all within 15% at three concentration levels. No matrix effect and carry-over effect were observed. Amygdalin and paeoniflorin were stable in human urine under different storage conditions. Approximately 79.6% of the administered amount of amygdalin was excreted unchanged in urine within 24 h and which was 48.4% for paeoniflorin. The developed LC-MS/MS method can be applied to evaluate the urinary excretion of amygdalin and paeoniflorin.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

26837182

Citation

Li, Xiao-bing, et al. "[An LC-MS/MS Method for the Simultaneous Determination of Amygdalin and Paeoniflorin in Human Urine and Application to Urinary Excretion Study]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 50, no. 10, 2015, pp. 1330-5.
Li XB, Shi FG, Jian LY, et al. [An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study]. Yao Xue Xue Bao. 2015;50(10):1330-5.
Li, X. B., Shi, F. G., Jian, L. Y., & Ding, L. (2015). [An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 50(10), pp. 1330-5.
Li XB, et al. [An LC-MS/MS Method for the Simultaneous Determination of Amygdalin and Paeoniflorin in Human Urine and Application to Urinary Excretion Study]. Yao Xue Xue Bao. 2015;50(10):1330-5. PubMed PMID: 26837182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [An LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in human urine and application to urinary excretion study]. AU - Li,Xiao-bing, AU - Shi,Fu-guo, AU - Jian,Ling-yan, AU - Ding,Li, PY - 2016/2/4/entrez PY - 2016/2/4/pubmed PY - 2016/2/13/medline SP - 1330 EP - 5 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 50 IS - 10 N2 - ABSTRACT: The study aims to develop an LC-MS/MS method for the simultaneous determination of amygdalin and paeoniflorin in urine samples, and to investigate their urinary excretion characteristics in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection (HTLPI). The urine samples were extracted by methanol, and then separated on a Hedera ODS-2 column with a mobile phase of acetonitrile and 5 mmol · L(-1) ammonium acetate buffer solution containing 0.05% formic acid (20:80). Electrospray ionization source was applied and operated in the positive ion mode using MRM. The method exhibited good linearity over the concentration range of 0.03 -40 µg · mL(-1). The values on both the occasions (intra- and inter-day) were all within 15% at three concentration levels. No matrix effect and carry-over effect were observed. Amygdalin and paeoniflorin were stable in human urine under different storage conditions. Approximately 79.6% of the administered amount of amygdalin was excreted unchanged in urine within 24 h and which was 48.4% for paeoniflorin. The developed LC-MS/MS method can be applied to evaluate the urinary excretion of amygdalin and paeoniflorin. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/26837182/[An_LC-MS/MS_method_for_the_simultaneous_determination_of_amygdalin_and_paeoniflorin_in_human_urine_and_application_to_urinary_excretion_study] DB - PRIME DP - Unbound Medicine ER -